Team:Melbourne/Safety

From 2014.igem.org

(Difference between revisions)
(Prototype team page)
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<td style="border:1px solid black;" colspan="3" align="center" height="150px" bgColor=#FF404B>
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<h1 >WELCOME TO iGEM 2014! </h1>
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}
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<p>Your team has been approved and you are ready to start the iGEM season!
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<br>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Melbourne/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<a href="https://2014.igem.org/Team:Melbourne"style="color:#000000">Home </a> </td>
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<a href="https://igem.org/Team.cgi?year=2014&team_name=Melbourne"style="color:#000000"> Official Team Profile </a></td>
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<a href="https://2014.igem.org/Team:Melbourne/Project"style="color:#000000"> Project</a></td>
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<a href="https://2014.igem.org/Team:Melbourne/Safety"style=" color:#000000"> Safety </a></td>
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<a href="https://2014.igem.org/Team:Melbourne/Attributions"style="color:#000000"> Attributions </a></td>
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<td > <h3> Timeline</h3></td>
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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Please use this page to write about anything related to safety in your project. <!--Be sure to talk about both
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<ul>
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<li> <a href=" ">Learn about lab Safety for Today</a></li>
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<li> <a href="">Learn about Safety for the future of your project.</a></li>
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<h3> Your Lab </h3>
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
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Image:Example2_Lab_2.png|The inside of our lab!
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Image:Example2_Lab_3.png|Team Member 3 doing an experiment
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Image:Example2_Lab_4.png|Working in biosafety cabinets
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Image:Example2_Lab_5.png|Team all gloved up and ready for work!
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Image:Example2_Lab_6.png|Equipment that we use to do SCIENCE!
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<ul>
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/**
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<li> <b>Now :</b> Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
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<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
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<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
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<li><b>June 9: </b>Submit the About Our Lab form.</li>
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<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
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<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
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<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
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<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
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<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
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<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
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Team </a> </td>
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Project</a></td>
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Parts</a></td>
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Modeling</a></td>
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<a href="https://2014.igem.org/Team:Melbourne/Notebook"style="color:#000000">
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Notebook</a></td>
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<td align="center"  height="30px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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<a href="https://2014.igem.org/Team:Melbourne/Safety"style=" color:#000000">
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Safety </a></td>
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<td align="center"  height="30px" onMouseOver="this.bgColor='#d3d3d3'" onMouseOut="this.bgColor='#e7e7e7'" bgColor=#e7e7e7>
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<a href="https://2014.igem.org/Team:Melbourne/Attributions"style="color:#000000">
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Attributions </a></td>
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<td align="center"> <a href="https://2014.igem.org/Main_Page"> <img src="https://static.igem.org/mediawiki/igem.org/6/60/Igemlogo_300px.png" width="55px"></a> </td>
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</tr>
</table>
</table>
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<h1 >University of Melbourne<br>
 +
iGEM team </h1>
 +
<p>Welcome, from the team at the University of Melbourne, Australia<br>
 +
<br>Contact us via email at melbourneuniigem@gmail.com<br>
 +
 +
</p>
 +
<!-- INSERT YOUR CONTENT HERE -->
 +
<br>
 +
1. Safety Level Rating System
 +
<br>
 +
Australia uses a four-part ‘Safety Level’ rating system for laboratories in which Level 4 is used for the most dangerous organisms. Our laboratory is Level 2 (moderate risk).
 +
 +
<br><br>
 +
2. Work Environments Used to Handle Biological Materials
 +
<br>
 +
Open benches and a laminar flow hood/biosafety cabinet with open front are used when handling biological materials in our laboratory.<br>
 +
 +
Some materials are handled in different work environments, for example DNA gels containing EtBr are only used in designated areas and some procedures involving bacteria that need to be sterile are performed near an open flame, while most other procedures are performed at open benches.
 +
 +
<br><br>
 +
3. Personal Protective Equipment
 +
<br>
 +
In our laboratory lab coats, gloves and safety glasses/goggles are worn at all times and full face shields are worn when using the UV light box. Latex gloves are used for most procedures in the lab, however, nitrile gloves are worn when handling EtBr.
 +
 +
<br><br>
 +
4. Disposal of Biological Waste
 +
<br>
 +
Biological waste (e.g. pipette tips) are disposed of in a yellow biohazard which is sealed when not in use. When full, this biohazard bin is sent to be autoclaved before it is disposed with appropriately along with the biological waste from the other laboratories in the institute.<br>
 +
 +
Biological waste such as liquid cell cultures has bleach added to it and sits overnight in the fume cabinet before the liquid is poured down a special biological waste drain and the container is autoclaved.
 +
<br><br>
 +
5. Safety Training
 +
<br>
 +
The Melbourne iGEM team received safety training provided by The Bio21 Institute. The topics covered in this training included: details of emergency actions, the university OH&S policy and issue resolution procedures, the university's environment policy and procedures, procedures for reporting incidents/near misses, hazards and unsafe acts/work processes, introduction to Bio21 EHS staff, location of relevant EHS documentation, security, 'out of normal hours' access, equipment operating procedures, general safety.<br>
 +
 +
The laboratory safety requirements of the institution can be found here: https://intranet.mdhs.unimelb.edu.au/ohse-training-requirements.<br>
 +
<br>
 +
6. Local Rules and Regulations
 +
<br>
 +
Christian Rantzau (Bio21 Health & Safety Representative) is responsible for biological safety at our institution. We briefly discussed the program with him and no concerns were raised, nor changes made to the project. He ensured that we were appropriately trained in the safety protocols and procedures of the institution.<br>
 +
 +
The biosafety guidelines of The Bio21 Institute can be found at: http://www.bio21.unimelb.edu.au/bio-21-institute-intranet/environmental-health-and-safety/safety.<br>
 +
 +
The regulations that govern biosafety in research laboratories in Australia may be found via the following link: http://www.ogtr.gov.au/internet/ogtr/publishing.nsf/Content/legislation-2.
 +
<br><br>
 +
7. Organisms and Parts Used
 +
<br>
 +
Species Name: E. coli, BL21(DE3) <br>
 +
Risk Group: 1 <br>
 +
Risk Group Source: E. coli K12 <br>
 +
Disease risk to humans?: Very low virulence <br>
 +
Part number/name: <br>
 +
Natural function of part: <br>
 +
How did you acquire it?: Donation from neighboring lab <br>
 +
How will you use it?: This is one of our chassis <br>
 +
<br>
 +
Species Name: E. coli, SHuffle T7 commercially available cells <br>
 +
Risk Group: 1 <br>
 +
Risk Group Source: E. coli B <br>
 +
Disease risk to humans?: Very low virulence <br>
 +
Part number/name: <br>
 +
Natural function of part: <br>
 +
How did you acquire it?: Acquired from local New England Biolabs agent <br>
 +
How will you use it?: This is one of our chassis <br>
 +
<br>
 +
Species Name: <br>
 +
Risk Group: <br>
 +
Risk Group Source: <br>
 +
Disease risk to humans?: <br>
 +
Part number/name: Synthetic peptide 1 based on a de novo amino acid sequence <br>
 +
Natural function of part: Not natural <br>
 +
How did you acquire it?: Designed DNA from scratch; DNA synthesised by Life Technologies <br>
 +
How will you use it?: <br>
 +
<br>
 +
Species Name: <br>
 +
Risk Group: <br>
 +
Risk Group Source: <br>
 +
Disease risk to humans?: <br>
 +
Part number/name: Synthetic peptide 2 based on a de novo amino acid sequence <br>
 +
Natural function of part: Not natural <br>
 +
How did you acquire it?: Designed DNA from scratch; DNA synthesised by Life Technologies <br>
 +
How will you use it?: <br>
 +
<br>
 +
Species Name: <br>
 +
Risk Group: <br>
 +
Risk Group Source: <br>
 +
Disease risk to humans?: <br>
 +
Part number/name: Synthetic peptide 3 based on a de novo amino acid sequence <br>
 +
Natural function of part: Not natural <br>
 +
How did you acquire it?: Designed DNA from scratch; DNA synthesised by Life Technologies <br>
 +
How will you use it?: <br>
 +
<br>
 +
Species Name: <br>
 +
Risk Group: <br>
 +
Risk Group Source: <br>
 +
Disease risk to humans?: <br>
 +
Part number/name: Synthetic peptide 4 based on a de novo amino acid sequence <br>
 +
Natural function of part: Not natural <br>
 +
How did you acquire it?: Designed DNA from scratch; DNA synthesised by Life Technologies <br>
 +
How will you use it?: <br>
 +
<br>
 +
Species Name: <br>
 +
Risk Group: <br>
 +
Risk Group Source: <br>
 +
Disease risk to humans?: <br>
 +
Part number/name: Synthetic peptide 5 based on a de novo amino acid sequence <br>
 +
Natural function of part: Not natural <br>
 +
How did you acquire it?: Designed DNA from scratch; DNA synthesised by Life Technologies <br>
 +
How will you use it?: <br>
 +
<br>
 +
Species Name: <br>
 +
Risk Group: <br>
 +
Risk Group Source: <br>
 +
Disease risk to humans?: <br>
 +
Part number/name: TEV protease gene <br>
 +
Natural function of part: Tobacco etch virus protease <br>
 +
How did you acquire it?: Donation from neighboring lab <br>
 +
How will you use it?:
 +
 +
<br><br>
 +
8. Risks of Our Project Now
 +
<br>
 +
Risk to the Safety and Health of Team Members/People Working in the Lab<br>
 +
E. coli has a low, but not non-existent virulence. For this reason, we wear gloves and lab coats and wash our hands when entering and leaving the labs. When analysing DNA agarose gels both the EtBr solution and the UV light box pose a risk to the health of our team members. In these specific cases we wear nitrile gloves when handling the gel and a face shield when operating the light box.<br>
 +
<br>
 +
Risk to the Safety and Health of the General Public<br>
 +
E. coli has a low, but not non-existent virulence. For this reason, we wear gloves and lab coats and wash our hands when entering and leaving the labs. This helps to reduce the risk to the safety and health of the general public.<br>
 +
<br>
 +
Risk to the Environment<br>
 +
When disposing of things such as liquid cell culture, we sit them overnight in the fume cabinet with some bleach in the,. In the morning we then pour the liquid down the shoot in the fume cabinet and autoclave the containers. We do not pour hazardous chemicals down the sink. All of these action minimise the risks to the environment.<br>
 +
<br>
 +
Risks to Security Through Malicious Mis-Use<br>
 +
Our project does not pose much of a risk in terms of malicious mis-use by others, but to try and prevent this we have key card access into both the building and the labs so that they cannot be accessed by the general public.<br>
 +
<br>
 +
Measures Taken to Reduce Risks<br>
 +
We employ safe lab practices (i.e. wearing gloves and lab coats), dispose of waste appropriately, use a baceterium (E. coli) with a very low virulence, and have key card access to the labs.
 +
<br><br>
 +
9. Risks of Our Project in the Future
 +
<br>
 +
If our project is successful it will allow people to use the molecules we make as a platform technology for making disulphide bonded peptides. These peptides could potentially be used for harmful purposes. We don’t currently have any design features to reduce the risk of someone using our project for harmful purposes. In the future if it were deemed necessary we could potentially try to limit the types of amino residues or groups that could be added to the arms of our peptide by native chemical ligation.<br>
 +
 +
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Revision as of 03:43, 16 October 2014

Home Team Official Team Profile Project Parts Modeling Notebook Safety Attributions

University of Melbourne
iGEM team

Welcome, from the team at the University of Melbourne, Australia

Contact us via email at melbourneuniigem@gmail.com


1. Safety Level Rating System
Australia uses a four-part ‘Safety Level’ rating system for laboratories in which Level 4 is used for the most dangerous organisms. Our laboratory is Level 2 (moderate risk).

2. Work Environments Used to Handle Biological Materials
Open benches and a laminar flow hood/biosafety cabinet with open front are used when handling biological materials in our laboratory.
Some materials are handled in different work environments, for example DNA gels containing EtBr are only used in designated areas and some procedures involving bacteria that need to be sterile are performed near an open flame, while most other procedures are performed at open benches.

3. Personal Protective Equipment
In our laboratory lab coats, gloves and safety glasses/goggles are worn at all times and full face shields are worn when using the UV light box. Latex gloves are used for most procedures in the lab, however, nitrile gloves are worn when handling EtBr.

4. Disposal of Biological Waste
Biological waste (e.g. pipette tips) are disposed of in a yellow biohazard which is sealed when not in use. When full, this biohazard bin is sent to be autoclaved before it is disposed with appropriately along with the biological waste from the other laboratories in the institute.
Biological waste such as liquid cell cultures has bleach added to it and sits overnight in the fume cabinet before the liquid is poured down a special biological waste drain and the container is autoclaved.

5. Safety Training
The Melbourne iGEM team received safety training provided by The Bio21 Institute. The topics covered in this training included: details of emergency actions, the university OH&S policy and issue resolution procedures, the university's environment policy and procedures, procedures for reporting incidents/near misses, hazards and unsafe acts/work processes, introduction to Bio21 EHS staff, location of relevant EHS documentation, security, 'out of normal hours' access, equipment operating procedures, general safety.
The laboratory safety requirements of the institution can be found here: https://intranet.mdhs.unimelb.edu.au/ohse-training-requirements.

6. Local Rules and Regulations
Christian Rantzau (Bio21 Health & Safety Representative) is responsible for biological safety at our institution. We briefly discussed the program with him and no concerns were raised, nor changes made to the project. He ensured that we were appropriately trained in the safety protocols and procedures of the institution.
The biosafety guidelines of The Bio21 Institute can be found at: http://www.bio21.unimelb.edu.au/bio-21-institute-intranet/environmental-health-and-safety/safety.
The regulations that govern biosafety in research laboratories in Australia may be found via the following link: http://www.ogtr.gov.au/internet/ogtr/publishing.nsf/Content/legislation-2.

7. Organisms and Parts Used
Species Name: E. coli, BL21(DE3)
Risk Group: 1
Risk Group Source: E. coli K12
Disease risk to humans?: Very low virulence
Part number/name:
Natural function of part:
How did you acquire it?: Donation from neighboring lab
How will you use it?: This is one of our chassis

Species Name: E. coli, SHuffle T7 commercially available cells
Risk Group: 1
Risk Group Source: E. coli B
Disease risk to humans?: Very low virulence
Part number/name:
Natural function of part:
How did you acquire it?: Acquired from local New England Biolabs agent
How will you use it?: This is one of our chassis

Species Name:
Risk Group:
Risk Group Source:
Disease risk to humans?:
Part number/name: Synthetic peptide 1 based on a de novo amino acid sequence
Natural function of part: Not natural
How did you acquire it?: Designed DNA from scratch; DNA synthesised by Life Technologies
How will you use it?:

Species Name:
Risk Group:
Risk Group Source:
Disease risk to humans?:
Part number/name: Synthetic peptide 2 based on a de novo amino acid sequence
Natural function of part: Not natural
How did you acquire it?: Designed DNA from scratch; DNA synthesised by Life Technologies
How will you use it?:

Species Name:
Risk Group:
Risk Group Source:
Disease risk to humans?:
Part number/name: Synthetic peptide 3 based on a de novo amino acid sequence
Natural function of part: Not natural
How did you acquire it?: Designed DNA from scratch; DNA synthesised by Life Technologies
How will you use it?:

Species Name:
Risk Group:
Risk Group Source:
Disease risk to humans?:
Part number/name: Synthetic peptide 4 based on a de novo amino acid sequence
Natural function of part: Not natural
How did you acquire it?: Designed DNA from scratch; DNA synthesised by Life Technologies
How will you use it?:

Species Name:
Risk Group:
Risk Group Source:
Disease risk to humans?:
Part number/name: Synthetic peptide 5 based on a de novo amino acid sequence
Natural function of part: Not natural
How did you acquire it?: Designed DNA from scratch; DNA synthesised by Life Technologies
How will you use it?:

Species Name:
Risk Group:
Risk Group Source:
Disease risk to humans?:
Part number/name: TEV protease gene
Natural function of part: Tobacco etch virus protease
How did you acquire it?: Donation from neighboring lab
How will you use it?:

8. Risks of Our Project Now
Risk to the Safety and Health of Team Members/People Working in the Lab
E. coli has a low, but not non-existent virulence. For this reason, we wear gloves and lab coats and wash our hands when entering and leaving the labs. When analysing DNA agarose gels both the EtBr solution and the UV light box pose a risk to the health of our team members. In these specific cases we wear nitrile gloves when handling the gel and a face shield when operating the light box.

Risk to the Safety and Health of the General Public
E. coli has a low, but not non-existent virulence. For this reason, we wear gloves and lab coats and wash our hands when entering and leaving the labs. This helps to reduce the risk to the safety and health of the general public.

Risk to the Environment
When disposing of things such as liquid cell culture, we sit them overnight in the fume cabinet with some bleach in the,. In the morning we then pour the liquid down the shoot in the fume cabinet and autoclave the containers. We do not pour hazardous chemicals down the sink. All of these action minimise the risks to the environment.

Risks to Security Through Malicious Mis-Use
Our project does not pose much of a risk in terms of malicious mis-use by others, but to try and prevent this we have key card access into both the building and the labs so that they cannot be accessed by the general public.

Measures Taken to Reduce Risks
We employ safe lab practices (i.e. wearing gloves and lab coats), dispose of waste appropriately, use a baceterium (E. coli) with a very low virulence, and have key card access to the labs.

9. Risks of Our Project in the Future
If our project is successful it will allow people to use the molecules we make as a platform technology for making disulphide bonded peptides. These peptides could potentially be used for harmful purposes. We don’t currently have any design features to reduce the risk of someone using our project for harmful purposes. In the future if it were deemed necessary we could potentially try to limit the types of amino residues or groups that could be added to the arms of our peptide by native chemical ligation.

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