"

From 2014.igem.org

---

Revision as of 03:36, 16 October 2014

Future Plans

Examination of more proteins

Like any new up and coming technique, the degron system, will require further testing with a larger variety of well studied mutant variants of a single protein as well as a larger number of well studied proteins in general before the system can truly be accepted.
Our current plans for the future are too test a protein 33RM2 and its less stable variant 33CL1 both of which are bind to PD-1 (a negative t-cell regulator that prevents the recognition of tumorous cells by the immune system). Since, the stability of both these proteins are known and have been verified using other techniques such as thermal melts, they are very suitable candidates for testing using our degron system.

Further evolving more stable variants of existing proteins

Throughout this past summer, out team has been evolving more stable variants of BINDI through error-prone PCR and going forwards we will continue this process and continually analyze the mutants with flow cytometry and select cells that exhibit higher fluorescence with FACS. Furthermore, our technique could be applied...
***********NEEDS TO BE FINISHED***************