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Team:Cambridge-JIC/Guide/Constructs/Reporters
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Revision as of 01:12, 16 October 2014
The following fluorescent proteins have been used with consistent results in MP: [Venus] [MRFP1] [eGFP].
A range of localisation tags can be added to enable to distinguish sources of expression and to visualise individual cells and nuclei. These include: [N7] [LTI].
These can be added using a [Linker] instead of the stop codon for the fluorescent protein.
These are clearly visible under fluorescent microscopes and confocal microscopy. Sample images include:
<img: LTI>
<img: N7>
Fluorescent transformants are visible first from 3-5 days after transformation.
[GUS] staining has been successfully used to characterise expression, as seen in:
