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Team:NTNU Trondheim/Project
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<h4 id="reporter"> Reporter BioBrick</h4> | <h4 id="reporter"> Reporter BioBrick</h4> | ||
| - | <p>The first stage of this project consists of creating a reporter BioBrick in order to confirm that we have are able to induce the expression of foreign genes in <a href="http://www.genome.jp/kegg-bin/show_organism?org=syn">< | + | <p>The first stage of this project consists of creating a reporter BioBrick in order to confirm that we have are able to induce the expression of foreign genes in <a href="http://www.genome.jp/kegg-bin/show_organism?org=syn"><i>Synechocystis</i> sp. PCC 6803</a>. This reporter BioBrick makes use of several other BioBricks, such as <a href="http://parts.igem.org/Part:BBa_J23101">BBa_J23101</a>, <a href="http://parts.igem.org/Part:BBa_B0034">BBa_B0034</a> and <a href="http://parts.igem.org/Part:BBa_C0012">BBa_C0012</a>.</p> |
<p>Once the reporter plasmid has been successfully constructed, we will verify that it is working as intended by inducing the expression of a fluorescent protein contained on the plasmid. Once induced, the colonies of cells transformed with this plasmid should exhibit a color that is determined by the fluorescent protein. </p> | <p>Once the reporter plasmid has been successfully constructed, we will verify that it is working as intended by inducing the expression of a fluorescent protein contained on the plasmid. Once induced, the colonies of cells transformed with this plasmid should exhibit a color that is determined by the fluorescent protein. </p> | ||
| - | <p>The plasmid is transformed into < | + | <p>The plasmid is transformed into <i>Synechocystis</i> sp. PCC 6803 by homologous recombination. Contained on the plasmid are left- and right flanking sequences obtained from the <i>Synechocystis</i> genome. These flanking sequences undergo homologous with their sister sequences in the genome of <i>Synechocystis</i>, integrating the plasmid sequence into the genome. </p> |
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<img src="https://static.igem.org/mediawiki/2014/4/41/NTNU_Trondheim_reporter_plasmid.png" width="400px"> | <img src="https://static.igem.org/mediawiki/2014/4/41/NTNU_Trondheim_reporter_plasmid.png" width="400px"> | ||
| - | <p> The reporter plasmid will use a fluorescent protein placed under the control of an indicuble promoter. This will allow us to easily verify that the plasmid works as intended inside < | + | <p> The reporter plasmid will use a fluorescent protein placed under the control of an indicuble promoter. This will allow us to easily verify that the plasmid works as intended inside <i>Synechocystis</i> sp. PCC 6803.</p> |
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Revision as of 12:20, 3 July 2014
WikitemplateB project
From 2014.igem.org
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Team Example's Project name! | ||||
Project IntroductionCO2 emissions have recieved a lot of attention in modern times, due to concerns that high emission levels are facilitating global warming. Consequently, a lot of research is focused on ways of reducing CO2 emissions from industry, and ways of fixating atmospheric CO at a greater than normal rate. Our project is attempting to produce a BioBrick, which when placed inside photosynthetic bacteria, increases their rate of CO2 fixation. In order to achieve this, we first need to construct a BioBrick that allows inducible expression of non native genes in our chassis; |
Results
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| Reporter BioBrick | CO2 fixation BioBrick | Testing CO2 fixation rate |
Reporter BioBrickThe first stage of this project consists of creating a reporter BioBrick in order to confirm that we have are able to induce the expression of foreign genes in Synechocystis sp. PCC 6803. This reporter BioBrick makes use of several other BioBricks, such as BBa_J23101, BBa_B0034 and BBa_C0012. Once the reporter plasmid has been successfully constructed, we will verify that it is working as intended by inducing the expression of a fluorescent protein contained on the plasmid. Once induced, the colonies of cells transformed with this plasmid should exhibit a color that is determined by the fluorescent protein. The plasmid is transformed into Synechocystis sp. PCC 6803 by homologous recombination. Contained on the plasmid are left- and right flanking sequences obtained from the Synechocystis genome. These flanking sequences undergo homologous with their sister sequences in the genome of Synechocystis, integrating the plasmid sequence into the genome. |
The reporter plasmid will use a fluorescent protein placed under the control of an indicuble promoter. This will allow us to easily verify that the plasmid works as intended inside Synechocystis sp. PCC 6803. |
