"
Team:Aix-Marseille/Parts
From 2014.igem.org
| Line 97: | Line 97: | ||
<h1>Favorite parts</h1> | <h1>Favorite parts</h1> | ||
| - | <div class=" | + | <div class="project-subsection"> |
<span class="project-tag" id="part001"></span> | <span class="project-tag" id="part001"></span> | ||
<h2 class="subtitle">Part BBa_K1349001</h3> | <h2 class="subtitle">Part BBa_K1349001</h3> | ||
<p><a href="" target="_blank">Link to registry</a></p> | <p><a href="" target="_blank">Link to registry</a></p> | ||
| - | + | <div class="parts-subsection"> | |
<h3>Name</h3> | <h3>Name</h3> | ||
<p>RelA-2stop</p> | <p>RelA-2stop</p> | ||
| Line 107: | Line 107: | ||
<h3>Description</h3> | <h3>Description</h3> | ||
<p>The <i>relA</i> gene encodes a ppGpp synthetase. In bacteria, ppGpp (guanosine 3'-diphosphate 5-' diphosphate) acts as a signaling molecule that regulates a variety of cellular metabolisms in response to changes in the nutritional state of the cells. The relA sequence amplified from <i>E. coli</i> W3110 was mutated by site-directed mutagenesis to remove the PstI site.</p> | <p>The <i>relA</i> gene encodes a ppGpp synthetase. In bacteria, ppGpp (guanosine 3'-diphosphate 5-' diphosphate) acts as a signaling molecule that regulates a variety of cellular metabolisms in response to changes in the nutritional state of the cells. The relA sequence amplified from <i>E. coli</i> W3110 was mutated by site-directed mutagenesis to remove the PstI site.</p> | ||
| - | </div> | + | </div></div> |
| - | <div class=" | + | <div class="project-subsection"> |
<span class="project-tag" id="part002"></span> | <span class="project-tag" id="part002"></span> | ||
<h2 class="subtitle">Part BBa_K1349002</h3> | <h2 class="subtitle">Part BBa_K1349002</h3> | ||
<p><a href="" target="_blank">Link to registry</a></p> | <p><a href="" target="_blank">Link to registry</a></p> | ||
| - | + | <div class="parts-subsection"> | |
<h3>Name</h3> | <h3>Name</h3> | ||
<p>CusR box</p> | <p>CusR box</p> | ||
| Line 119: | Line 119: | ||
<h3>Description</h3> | <h3>Description</h3> | ||
<p>The CusR box allow the binding of the CusR regulator. In <i>E. coli</i>, the CusR/CusS two-component system induces expression of genes involved in metal efflux under condition of elevated Cu concentration. Phosphorylated CusR specifically bind to CusR-dependent promoters.</p> | <p>The CusR box allow the binding of the CusR regulator. In <i>E. coli</i>, the CusR/CusS two-component system induces expression of genes involved in metal efflux under condition of elevated Cu concentration. Phosphorylated CusR specifically bind to CusR-dependent promoters.</p> | ||
| - | </div> | + | </div></div> |
</div> | </div> | ||
Revision as of 22:41, 15 October 2014
Our parts
Favorite parts
Part BBa_K1349001
Name
RelA-2stop
Description
The relA gene encodes a ppGpp synthetase. In bacteria, ppGpp (guanosine 3'-diphosphate 5-' diphosphate) acts as a signaling molecule that regulates a variety of cellular metabolisms in response to changes in the nutritional state of the cells. The relA sequence amplified from E. coli W3110 was mutated by site-directed mutagenesis to remove the PstI site.
Part BBa_K1349002
Name
CusR box
Description
The CusR box allow the binding of the CusR regulator. In E. coli, the CusR/CusS two-component system induces expression of genes involved in metal efflux under condition of elevated Cu concentration. Phosphorylated CusR specifically bind to CusR-dependent promoters.
Other parts
Part BBa_K1349000 - SerA_mut
Part BBa_K1349003 - P-cusR
Part BBa_K1349004 - Mesh1-stop
Part BBa_K1349005 - (SH3)x1
Part BBa_K1349006 - CheA-stop
Part BBa_K1349007 - CusR-LZA
Part BBa_K1349008 - (SH3pep)-LZA
