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Team:UFAM Brazil/7-9-2014
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| - | The transformation with pUC72 using approximately 1. | + | The transformation with pUC72 using approximately 1.000 ng/ul and 1 ng/ul worked!! But our transformation with the DNA diluted from the Kit Parts 2013 (BBa_E0840) didn’t grow any transforming cells!!! =( |
</p> | </p> | ||
| - | <p> Today we decided to work on the transformation once again with <i>Escherichia coli</i> DH5α RR1 using Transformation Efficiency Kit as a control and the DNA diluted from the | + | <p> Today we decided to work on the transformation once again with <i>Escherichia coli</i> DH5α RR1 using Transformation Efficiency Kit as a control and the DNA diluted from the DNAa Parts Kit 2013 (BBa_E0840), using iGEM’s standard transformation protocol. We have to be very careful!!!!</p> |
| - | <p> We had a big meeting with | + | <p> We had a big meeting with all our team members.</p> |
</td></tr> | </td></tr> | ||
Revision as of 21:06, 15 October 2014
07/09/2014 | ||
The transformation with pUC72 using approximately 1.000 ng/ul and 1 ng/ul worked!! But our transformation with the DNA diluted from the Kit Parts 2013 (BBa_E0840) didn’t grow any transforming cells!!! =( Today we decided to work on the transformation once again with Escherichia coli DH5α RR1 using Transformation Efficiency Kit as a control and the DNA diluted from the DNAa Parts Kit 2013 (BBa_E0840), using iGEM’s standard transformation protocol. We have to be very careful!!!! We had a big meeting with all our team members. | ||
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