Team:ITESM-CEM/Team/School

From 2014.igem.org

(Difference between revisions)
Line 311: Line 311:
<h2> iGEM ITESM-CEM Team</h2>
<h2> iGEM ITESM-CEM Team</h2>
-
<p style="text-align: justify; text-justify: inter-word;">Our school its located in Atizapán de Zaragoza, Estado de México, México</p>
+
<p style="text-align: justify; text-justify: inter-word;">ted into the tube, which was placed on ice for 30 minutes. Afterwards, the samples were submitted to 30 seconds of a 42°C heat shock; after which they were placed on ice for another 5 minutes. After incubation on ice, 950 μl of SOC medium were added to each mixture. <br>The tubes were placed at 37°C and 250 rpm for 60 minutes. Finally, 200 μl of each sample were plated into warm, solid LB media with 0.1% v/v of antibiotic (kanamycin 15 mg/ml for device 1, and chloramphenicol 35 mg/ml for devices 2, and 3)</p>
<br>
<br>
<br>
<br>

Revision as of 20:59, 15 October 2014

TEC-CEM | Team

ITESM-CEM | Enzy7-K me

Team 2100
 

iGEM ITESM-CEM Team

ted into the tube, which was placed on ice for 30 minutes. Afterwards, the samples were submitted to 30 seconds of a 42°C heat shock; after which they were placed on ice for another 5 minutes. After incubation on ice, 950 μl of SOC medium were added to each mixture.
The tubes were placed at 37°C and 250 rpm for 60 minutes. Finally, 200 μl of each sample were plated into warm, solid LB media with 0.1% v/v of antibiotic (kanamycin 15 mg/ml for device 1, and chloramphenicol 35 mg/ml for devices 2, and 3)

















Retrieved from "http://2014.igem.org/Team:ITESM-CEM/Team/School"