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Team:Brasil-SP/Project/DetectionModule
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<h3 align="center">Detection Module</h3> | <h3 align="center">Detection Module</h3> | ||
| - | <p><div align="justify"> The genetic circuit that is being developed by the Brasil-SP team is a biodetection system designed for Cystatin C (Cys C). We will use the Quorum Sensing bacterial recognition system, based on communication between bacteria. This system consists in the recognition and release of substances that diffuse through the environment; those substances are called autoinducers. The autoinducers are responsible for the activation of their own synthesis, allowing the bacterial cells to respond appropriately to cell density. Thus, it is possible to control the expression of specific genes that are only activated when a certain cell concentration is achieved; as a result the behavior of the group and the formation of communities can also be controlled.</p> | + | <p><div align="justify"> The genetic circuit that is being developed by the Brasil-SP team is a biodetection system designed for Cystatin C (Cys C). We will use the Quorum Sensing bacterial recognition system (QS), based on communication between bacteria. This system consists in the recognition and release of substances that diffuse through the environment; those substances are called autoinducers. The autoinducers are responsible for the activation of their own synthesis, allowing the bacterial cells to respond appropriately to cell density. Thus, it is possible to control the expression of specific genes that are only activated when a certain cell concentration is achieved; as a result the behavior of the group and the formation of communities can also be controlled.</p> |
<p><div align="justify"> In our biosensor, the Quorum Sensing substance is called AIP and its receiver is called ComD. Both AIP and ComD are anchored in the cell membrane. The AIP is attached to a linker that is cleaved in the presence of the protease cathepsin S, which can be inhibited by Cystatin C activity. After being cleaved and released from the membrane, AIP binds to the receptor triggering the phosphorylation of ComE (intracellular signalling molecule), which binds to a specific promoter sensitive to ComE, initiating the expression of the downstream gene (lasR). The Cys C concentrations will be detected through the variation of the cathepsin S protease activity, which ultimately causes regulates LasR production. In the Diagnosis section we describe how the bacteria will be capable of processing this information to discriminate between normal and increased Cys C concentration. </p> | <p><div align="justify"> In our biosensor, the Quorum Sensing substance is called AIP and its receiver is called ComD. Both AIP and ComD are anchored in the cell membrane. The AIP is attached to a linker that is cleaved in the presence of the protease cathepsin S, which can be inhibited by Cystatin C activity. After being cleaved and released from the membrane, AIP binds to the receptor triggering the phosphorylation of ComE (intracellular signalling molecule), which binds to a specific promoter sensitive to ComE, initiating the expression of the downstream gene (lasR). The Cys C concentrations will be detected through the variation of the cathepsin S protease activity, which ultimately causes regulates LasR production. In the Diagnosis section we describe how the bacteria will be capable of processing this information to discriminate between normal and increased Cys C concentration. </p> | ||
Revision as of 20:32, 15 October 2014
Detection Module
The genetic circuit that is being developed by the Brasil-SP team is a biodetection system designed for Cystatin C (Cys C). We will use the Quorum Sensing bacterial recognition system (QS), based on communication between bacteria. This system consists in the recognition and release of substances that diffuse through the environment; those substances are called autoinducers. The autoinducers are responsible for the activation of their own synthesis, allowing the bacterial cells to respond appropriately to cell density. Thus, it is possible to control the expression of specific genes that are only activated when a certain cell concentration is achieved; as a result the behavior of the group and the formation of communities can also be controlled.
In our biosensor, the Quorum Sensing substance is called AIP and its receiver is called ComD. Both AIP and ComD are anchored in the cell membrane. The AIP is attached to a linker that is cleaved in the presence of the protease cathepsin S, which can be inhibited by Cystatin C activity. After being cleaved and released from the membrane, AIP binds to the receptor triggering the phosphorylation of ComE (intracellular signalling molecule), which binds to a specific promoter sensitive to ComE, initiating the expression of the downstream gene (lasR). The Cys C concentrations will be detected through the variation of the cathepsin S protease activity, which ultimately causes regulates LasR production. In the Diagnosis section we describe how the bacteria will be capable of processing this information to discriminate between normal and increased Cys C concentration.
