Team:USTC-China/safety

From 2014.igem.org

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           <a href="#panel1">Safety</a>
           <a href="#panel1">Safety</a>
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          <ul class="side-nav">
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          <a href="https://2014.igem.org/Team:USTC-China/safety/background">
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          Background
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          <a href="https://2014.igem.org/Team:USTC-China/safety/details">
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          Details
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          <a href="https://2014.igem.org/Team:USTC-China/safety/results">
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          Results
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                <h2 id="safetytraininginourteam">Safety Training in our team</h2>
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            NULL
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    <h2 id="killswitchinecoli">Kill Switch in <em>E.coli</em></h2>
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    <p>This year our safety work is based on <em>E.coli</em>, since some of our work is on light sensing and imaging system in <em>E.coli</em>. To make a wider application in the future, the biosafety of <em>E.coli</em> containing these parts should be concerned.</p>
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    <p>LALF is </p>
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    <h3 id="antilpsfactorlalfregulatedbylaci">Anti-LPS factor(LALF) regulated by lacI</h3>
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    <p>We designed the part based on K541545. We found that R0010 might keep express genes below without regulation.Reason may be that Lacl expressed by E.coil is limited. So we inserted parts that express Lacl and constitutive promoter before the old one to inhibit pLac. <br />
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    <img src="https://static.igem.org/mediawiki/2014/c/c3/844.png" alt="" /></p>
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    <h3 id="antilpsfactorlalfregulatedbyiptg">Anti-LPS factor(LALF) regulated by IPTG</h3>
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    <p>This part is a cell-kill system. Firstly,constitutive promoter could express enough Lacl that is useful for downstream. Secondly, the reporter in the middle of our part wouldn't show signal nor the cell be killed by the part behind unless IPTG or lactose added into the culture medium. No IPTG or lactose: GFP don't express ,G- won't be killed. IPTG or lactose existing : GFPs express ,G- will be dead. </p>
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    <p>G-: Gram negative bacteria <br />
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    <img src="https://static.igem.org/mediawiki/2014/e/e2/045.png" alt="" /></p>
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     </div>
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Revision as of 18:30, 15 October 2014

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