Team:Arizona State/teamprofile

From 2014.igem.org

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   <p><strong>Ethanol Production Parts</strong> <br>
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   <p><strong>Name</strong> <br>
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     To optimize ethanol production in our EtOH strain, we wanted to overexspress the pyruvate decarboxylase (pdc) and alcohol dehydrogenase B(adhB) enzymes in order to maximize the amount of ethanol being produced. The iGEM registry contained the part BBa_k1122676, made by Edinburgh in 2013. This part was compared to another plasmid containing pdc/adhB, donated by Dr. David Neilsen. The results indiciated that the BBa_k1122676 was the better ethanol producer, making it the key component of the EtOH strain. Registry page: http://parts.igem.org/Part:BBa_K1122676</p>
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   <p><strong>Fatty Acid Production Parts</strong> <br>
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     In order to maximize the amount of fatty acetyl-coenzyme A molecules being produced in the fatty acid strain, two enzymes were targeted for overproduction. The first was the thioesterase A (TesA) protein, which takes the fatty Acyl-ACP produced from glycolysis and produces free fatty acid chains. The part BBa_K654058 codes for this protein, but it does not have a promoter or an RBS.  </p>
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   <p><strong>Biodesiel Synthesis Parts</strong> <br>
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     Consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat </p>
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Revision as of 18:23, 15 October 2014


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The Arizona State University iGEM team


 

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