Team:Hannover/Results/Heavy Metals/Arabidopsis

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<h1>Results / Heavy metals / <i>Arabidopsis thaliana</i> T4MBP</h1>
<h1>Results / Heavy metals / <i>Arabidopsis thaliana</i> T4MBP</h1>
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<h2>Labwork</h2><p class="text">Transformation of <i>A. thaliana</i> with our T4MBP:<ol><li><b></b>Exchange the original promoter enTCUP2 of the binary vector pORE_E3 <a href="http://www.ncbi.nlm.nih.gov/nuccore/AY562536">(AY562536.1)</a> into a 2x35s promoter using <a href="">this protocol.</a></li><li>We integrated our T4MBP in our modified pORE_E3_2x35S.</li><li>Application of our <a href="">floral dip </a>method.</li><li>After 6 weeks we harvested transgenic seeds from <i>A. thaliana</i> and plated these seeds on selection MSO-media.</li><li>At last we potted transformed plants.</li></ol></p>
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<h2>Labwork</h2><p class="text">Transformation of <i>A. thaliana</i> with our T4MBP:<ol><li><b></b>Exchange the original promoter enTCUP2 of the binary vector pORE_E3 <a href="http://www.ncbi.nlm.nih.gov/nuccore/AY562536">(AY562536.1)</a> into a 2x35s promoter using <a href="">this protocol.</a></li><li>We integrated our T4MBP in our modified pORE_E3_2x35S.</li><li>Application of our <a href="https://2014.igem.org/Team:Hannover/Protocols/Transformation/Arabidopsis#">floral dip </a>method.</li><li>After 6 weeks we harvested transgenic seeds from <i>A. thaliana</i> and plated these seeds on selection MSO-media.</li><li>At last we potted transformed plants.</li></ol></p>
<h2>Results</h2>
<h2>Results</h2>

Revision as of 17:55, 15 October 2014

Results / Heavy metals / Arabidopsis thaliana T4MBP

Labwork

Transformation of A. thaliana with our T4MBP:

  1. Exchange the original promoter enTCUP2 of the binary vector pORE_E3 (AY562536.1) into a 2x35s promoter using this protocol.
  2. We integrated our T4MBP in our modified pORE_E3_2x35S.
  3. Application of our floral dip method.
  4. After 6 weeks we harvested transgenic seeds from A. thaliana and plated these seeds on selection MSO-media.
  5. At last we potted transformed plants.

Results

The exchange of the promoter was checked by sequencing. We achieved to regenerate young A. thaliana after the transformation with our T4MBP.

Fig. 1: Pictures showing young potentially transgenic A. thaliana before, during and after the transfer from medium to earth.

Fig. 2: Pictures showing the vector pORE_E3 with 2x35S promoter and the way of exchanging the enTCUP2 promoter with the 2x35S promoter.





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