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| | <div id="innercontenthome"> | | <div id="innercontenthome"> |
| | <div class="centering" style="padding-top: 85px; padding-bottom:40px;"> | | <div class="centering" style="padding-top: 85px; padding-bottom:40px;"> |
| - |
| |
| - | <div id="column-left">
| |
| - | <h3 class="title2" style="margin-top:10px; color:#333;">Summary :</h3>
| |
| - | <ul class="menuleft">
| |
| - | <li><a href="#select1">BBa_K1364000</a></li>
| |
| - | <li><a href="#select2">BBa_K1364004</a></li>
| |
| - | <li><a href="#select3">BBa_K1364005</a></li>
| |
| - | <li><a href="#select4">BBa_K1364002</a></li>
| |
| - | <li><a href="#select5">BBa_K1364007</a></li>
| |
| - | <li><a href="#select6">BBa_K1364008</a></li>
| |
| - | <li><a href="#select7">BBa_K1364003</a></li>
| |
| - | <li><a href="#select8">BBa_K1364009</a></li>
| |
| - | <li><a href="#select9">BBa_K1364010</a></li>
| |
| - | <li><a href="#select10">BBa_K1364011</a></li>
| |
| - | <li><a href="#select11">BBa_K1364013</a></li>
| |
| - | <li><a href="#select12">BBa_K1364015</a></li>
| |
| - | <li><a href="#select13">BBa_K1364016</a></li>
| |
| - | <li><a href="#select14">BBa_K1364017</a></li>
| |
| - | <li><a href="#select15">BBa_K1364019</a></li>
| |
| - | <li><a href="#select16">BBa_K1364021</a></li>
| |
| - | </ul>
| |
| - | </div>
| |
| - |
| |
| - | <div class="column-right" style="width:75%; float:right;">
| |
| | | | |
| | <p class="texte">As the chassis <i>B. subtilis</i> is not as used as <i>E. coli</i>, | | <p class="texte">As the chassis <i>B. subtilis</i> is not as used as <i>E. coli</i>, |
| Line 176: |
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| | | | |
| | <p class="title1"><a href="https://2014.igem.org/Team:Toulouse/Result/parts/Submitted_parts">Submitted parts</p></a> | | <p class="title1"><a href="https://2014.igem.org/Team:Toulouse/Result/parts/Submitted_parts">Submitted parts</p></a> |
| | + | <p class="title1"><a href="https://2014.igem.org/Team:Toulouse/Result/parts/Used_parts">Used parts</p></a> |
| | | | |
| - | <p class="texte">
| |
| - | We have deposed 16 new BioBrick parts to the Registry.
| |
| - | All of them were cloned into the standard plasmid pSB1C3, tested and sequenced.<br>
| |
| - | <!--à vérifier et confirmer-->
| |
| - | </p>
| |
| - |
| |
| - |
| |
| - | <p class="title2">I. Chemotaxis</p>
| |
| - |
| |
| - | <!--K1364000 : RBS + Chimio + terminator-->
| |
| - |
| |
| - | <p class="title3" id="select1">
| |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364000">BBa_K1364000</a>: N-acetylglucosamine based chemotaxis for <i>Bacillus subtilis</i><br></p>
| |
| - | <p class="texte">This part is designed to enable a N-acetylglucosamine
| |
| - | based chemotaxis in <i>Bacillus subtilis</i>.
| |
| - | <p class="title4">Design</p>
| |
| - | <p class="texte">The encoded protein is a chimera of two proteins:<br>
| |
| - | - the methyl accepting Chemotaxis protein (McpA from <i>Bacillus subtilis</i> which is required for taxis towards glucose.<br>
| |
| - | - the N-acetylglucosamine regulated methyl-accepting chemotaxis protein from <i>Vibrio cholerae</i> (VCD).<br>
| |
| - | <br>
| |
| - | The chimeric protein contains the intracellular domains of McpA from <i>Bacillus subtilis</i> to enable the transduction of the signal:
| |
| - | these domains correspond to the amino acids 1-37 and 282-610.
| |
| - | The extracellular domain of VCD is inserted between the intracellular regions of McpA
| |
| - | to sense N-acetylglucosamine and corresponds to the amino acids 31-310.<br></p>
| |
| - | <img src="https://static.igem.org/mediawiki/2014/a/a2/BBa_K1364000.png">
| |
| - | <p class="title4">Type</p>
| |
| - | <p class="texte">Translation unit</p>
| |
| - | <p class="title4">Tests</p>
| |
| - | <p class="texte">This part is not tested yet.</p>
| |
| - | <p class="title4">References</p>
| |
| - | <p class="texte">Uniprot:<br>
| |
| - | - <a href="http://www.uniprot.org/uniprot/P39214">McpA</a><br>
| |
| - | - <a href="http://www.uniprot.org/uniprot/C3NYT2">N-acetylglucosamine regulated methyl-accepting chemotaxis protein</a><br></p>
| |
| - | <!--à compléter-->
| |
| - | </p>
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <!--K1364004 : Promotor Pveg + Chimio-->
| |
| - | <p class="title3" id="select2">
| |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364004">BBa_K1364004</a>: P<sub>veg</sub> + N-acetylatedglucosamine based chemotaxis for <i>B. subtilis</i>
| |
| - | <br>
| |
| - | <p class="texte">This part is designed to enable a N-acetylglucosamine based chemotaxis in <i>Bacillus subtilis</i>.</p>
| |
| - | <p class="title4">Design</p>
| |
| - | <p class="texte">This expression cassette is designed for the expression of an antifungal peptide,
| |
| - | D4E1 and for its secretion in <i>Bacillus subtilis</i>.
| |
| - | It is composed of the strong, constitutive promotor of <i>B. subtilis</i>
| |
| - | P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
| |
| - | strong RBS for <i>B. subtilis</i> (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
| |
| - | the open reading frame of D4E1
| |
| - | and a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>)</p>
| |
| - | <img src="https://static.igem.org/mediawiki/2014/3/35/BBa_K1364004.png">
| |
| - | <p class="title4">Type</p>
| |
| - | <p class="texte">Generator</p>
| |
| - | <p class="title4">Tests</p>
| |
| - | <p class="texte">This part was not tested yet.</p>
| |
| - | <p class="title4">References</p>
| |
| - | <p class="texte">Uniprot:<br>
| |
| - | - <a href="http://www.uniprot.org/uniprot/P39214">McpA</a><br>
| |
| - | - <a href="http://www.uniprot.org/uniprot/C3NYT2">N-acetylglucosamine regulated methyl-accepting chemotaxis protein</a><br>
| |
| - | </p>
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <p class="title2">II. Binding</p>
| |
| - |
| |
| - | <!--K1364001 : RBS + Binding + Terminator-->
| |
| - | <p class="title3" id="select3">
| |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364005">BBa_K1364005</a>: P<sub>veg</sub> + Chitin Binding Cell Wall protein</p>
| |
| - | <p class="texte">This part is designed to enable the binding of <i>Bacillus subtilis</i> to the fungi wall made of chitin.<p/>
| |
| - | <p class="title4">Design</p>
| |
| - | <p class="texte">This part is composed of a Cell Wall Binding (CWB) sequence
| |
| - | and a Chitin Binding Domain (CBD) linked with a 6 amino acids linker.<br>
| |
| - | <br>
| |
| - | The CWB domain has been extracted from LytC (<a href="http://parts.igem.org/Part:BBa_K316030">BBa_K316030</a>) of the iGEM 2010 Imperial College team.<br>
| |
| - | The CBD is composed of the Domain 4 of the GbpA protein of <i>Vibrio cholerae</i>,
| |
| - | a protein reported to mediate bacterial attachment to chitin.
| |
| - | It has been shown that the Domain 4 was essential to bind chitin.</p>
| |
| - | <img src="https://static.igem.org/mediawiki/2014/f/f5/BBa_K1364005.png">
| |
| - | <p class="title4">Type</p>
| |
| - | <p class="texte">Generator</p>
| |
| - | <p class="title4">Tests</p>
| |
| - | <p class="texte">This part was tested with chitin beads. (See <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results">Binding module</a>)</p>
| |
| - | <p class="title4">References</p>
| |
| - | <p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
| |
| - | - The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br>
| |
| - | </p>
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - |
| |
| - | <p class="title2"> III. Fungicides</p>
| |
| - | <p class="title3">GAFP-1</p>
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <!--K1364002 : RBS + GAFP-1-->
| |
| - | <p class="title3" id="select4">
| |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</a>: RBS - Antifungal GAFP-1</p>
| |
| - | <p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin, is a mannose and chitin binding lectin
| |
| - | originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
| |
| - | GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
| |
| - | <p class="title4">Design</p>
| |
| - | <p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>) and
| |
| - | the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
| |
| - | optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
| |
| - | The codon optimization was made thanks to the DNA 2.0 software program.</p>
| |
| - | <img src="https://static.igem.org/mediawiki/2014/5/5f/BBa_K1364002.png">
| |
| - | <p class="title4">Type</p>
| |
| - | <p class="texte">Translational unit</p>
| |
| - | <p class="title4">Tests</p>
| |
| - | <p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
| |
| - | <p class="title4">References</p>
| |
| - | <p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
| |
| - | - The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br></p>
| |
| - | <br>
| |
| - |
| |
| - | <!--K1364007 : RBS + GAFP-1 + Terminator-->
| |
| - | <p class="title3" id="select5">
| |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364007">BBa_K1364007</a>: RBS + Antifungal GAFP-1 + Double terminator</p>
| |
| - | <p class="texte">The <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin, is a mannose and chitin binding lectin
| |
| - | originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
| |
| - | GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
| |
| - | <p class="title4">Design</p>
| |
| - | <p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
| |
| - | the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
| |
| - | and a double terminator <a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>.
| |
| - | optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
| |
| - | The codon optimization was made thanks to the DNA 2.0 software program.</p>
| |
| - | <img src="https://static.igem.org/mediawiki/2014/4/42/BBa_K1364007.png">
| |
| - | <p class="title4">Type</p>
| |
| - | <p class="texte">Composite part</p>
| |
| - | <p class="title4">Tests</p>
| |
| - | <p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
| |
| - | <p class="title4">References</p>
| |
| - | <p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
| |
| - | - The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br></p>
| |
| - | <br>
| |
| - |
| |
| - | <!--K13640008 : Pveg + RBS + GAFP-1 + Terminator-->
| |
| - | <p class="title3" id="select6">
| |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364008">BBa_K1364008</a>: P<sub>veg</sub> - strong RBS - Antifungal GAFP-1 - Double terminator</p>
| |
| - | <p class="texte">This expression cassette is designed for the expression and secretion of the <i>Gastrodia</i> anti-fungal protein(GAFP-1), also known as gastrodianin. GAFP-1 is a mannose and chitin binding lectin
| |
| - | originating from the Asiatic orchid Gastrodia elata, a traditional Chinese medicinal herb cultured for thousands of years.
| |
| - | GAFP-1 is composed of 15 amino acids LDSLSFSYNNFEEDD and is able to inhibit the growth of multiples species of plant pathogenic fungi.</p>
| |
| - | <p class="title4">Design</p>
| |
| - | <p class="texte">This part is composed of the strong, constitutive promoter of <i>Bacillus subtilis</i> P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
| |
| - | strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
| |
| - | the open reading frame of the <i>Gastrodia</i> anti-fungal protein 1 (GAFP-1)
| |
| - | and a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).
| |
| - | optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
| |
| - | The codon optimization was made thanks to the DNA 2.0 software program.</p>
| |
| - | <img src="https://static.igem.org/mediawiki/2014/6/67/BBa_K1364008.png">
| |
| - | <p class="title4">Type</p>
| |
| - | <p class="texte">Generator</p>
| |
| - | <p class="title4">Tests</p>
| |
| - | <p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
| |
| - | <p class="title4">References</p>
| |
| - | <p class="texte">- Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, et al. (2012)<br>
| |
| - | - The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces. PLoS Pathog 8(1): e1002373. doi:10.1371/journal.ppat.1002373<br>
| |
| - | </p>
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <p class="title3">D4E1</p>
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <!--K1364003 : RBS + D4E1 + Terminator-->
| |
| - | <p class="title3" id="select7">
| |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</a>:RBS - Antifungal D4E1 - Double terminator</p>
| |
| - | <p class="texte">D4E1 is a linear synthetic peptide of 17 amino acids
| |
| - | which has shown to have antifungal activities
| |
| - | by complexing with a sterol present in conodial wall of a varety of fungi.</p>
| |
| - | <p class="title4">Design</p>
| |
| - | <p class="texte">This part is composed of a Strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
| |
| - | the open reading frame of D4E1 and
| |
| - | a Double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>)
| |
| - | optimized for its expression and its secretion in <i>Bacillus subtilis</i>.
| |
| - | This part was optimized for the expression and its secretion in <i>Bacillus subtilis </i>
| |
| - | thanks to the DNA 2.0 software program.</p>
| |
| - | <img src="https://static.igem.org/mediawiki/2014/b/bb/BBa_K1364003.png">
| |
| - | <p class="title4">Type</p>
| |
| - | <p class="texte">Translational unit</p>
| |
| - | <p class="title4">Tests</p>
| |
| - | <p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
| |
| - | <p class="title4">References</p>
| |
| - | <p class="texte">- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.<br>
| |
| - | - Can J Microbiol. 1998 Jun;44(6):514-20.</p>
| |
| - |
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <!--K1364009 : Pveg + RBS + D4E1 + Terminator-->
| |
| - | <p class="title3" id="select8">
| |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364009">BBa_K1364009</a>:P<sub>veg</sub> - RBS - Antifungal D4E1 - Double Terminator</p>
| |
| - | <p class="texte">
| |
| - | D4E1 is a linear synthetic peptide of 17 amino acids
| |
| - | which has shown to have antifungal activities
| |
| - | by complexing with a sterol present in conodial wall of a varety of fungi.</p>
| |
| - | <p class="title4">Design</p>
| |
| - | <p class="texte">This part is composed of the strong, constitutive promotor of <i>B. subtilis</i>
| |
| - | P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>),
| |
| - | a strong RBS (<a href="http://parts.igem.org/Part:BBa_K780002">K780002</a>),
| |
| - | the open reading frame of D4E1 and
| |
| - | a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).
| |
| - | This part was optimized for the expression and its secretion in <i>Bacillus subtilis </i>
| |
| - | thanks to the DNA 2.0 software program.</p>
| |
| - | <img src="https://static.igem.org/mediawiki/2014/4/47/BBa_K1364009.png">
| |
| - | <p class="title4">Type</p>
| |
| - | <p class="texte">Translational unit</p>
| |
| - | <p class="title4">Tests</p>
| |
| - | <p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
| |
| - | <p class="title4">References</p>
| |
| - | <p class="texte">- De Lucca AJ, Bland JM, Grimm C, Jacks TJ, Cary JW, Jaynes JM, Cleveland TE, Walsh TJ. Fungicidal properties, sterol binding, and proteolytic resistance of the synthetic peptide D4E1.<br>
| |
| - | - Can J Microbiol. 1998 Jun;44(6):514-20.</p>
| |
| - | <br>
| |
| - |
| |
| - | <p class="title3">EcAMP-1</p>
| |
| - |
| |
| - | <!--K1364010 : Pveg RBS + EcAMP -->
| |
| - | <p class="title3" id="select9">
| |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364010">BBa_K1364010</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1</p>
| |
| - | <p class="texte">
| |
| - | EcAMP-1 is an antimicrobial peptide of 37 amino acids
| |
| - | originated from the specie <i>Echinochloa crus-galli</i>,
| |
| - | a type of wild grass.
| |
| - | This peptide has a particular structure : it is helical because of two disulfide bonds.<br>
| |
| - | EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.<br>
| |
| - | This part was added to the Registry by the iGEM Utah State team in 2013.</p>
| |
| - | <p class="title4">Design</p>
| |
| - | <p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
| |
| - | strong RBS for <i>B. subtilis</i>
| |
| - | (<a href="http://parts.igem.org/Part:BBa_K733013">K733013</a>) and
| |
| - | the open reading frame of EcAMP-1.<br>
| |
| - | The EcAMP-1 part was codon optimized for <i>E. coli</i> by the iGEM Utah State team
| |
| - | and thanks to the Life Technologies GeneArt software program. </p>
| |
| - | <img src="https://static.igem.org/mediawiki/2014/5/5f/BBa_K1364010.png">
| |
| - | <p class="title4">Type</p>
| |
| - | <p class="texte"> Generator</p>
| |
| - | <p class="title4">Tests</p>
| |
| - | <p class="texte">This part is not tested yet.</p>
| |
| - | <p class="title4">References</p>
| |
| - | <p class="texte"><!--à compléter : demander à emeline--></p>
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <!--K1364011 : Pveg RBS + EcAMP + Double term-->
| |
| - | <p class="title3">
| |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364011">BBa_K1364011</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</p>
| |
| - | <p class="texte" id="select10">
| |
| - | EcAMP-1 is an antimicrobial peptide of 37 amino acids
| |
| - | originated from the specie <i>Echinochloa crus-galli</i>,
| |
| - | a type of wild grass.
| |
| - | This peptide has a particular structure : it is helical because of two disulfide bonds.<br>
| |
| - | EcAMP-1 has shown to have antifungal activities. Its mode of action may be the prevention of hyphae elongation.<br>
| |
| - | This part was added to the Registry by the iGEM Utah State team in 2013.</p>
| |
| - | <p class="title4">Design</p>
| |
| - | <p class="texte">This part is composed of the constitutive promoter P<sub>veg</sub> and
| |
| - | strong RBS for <i>B. subtilis</i>
| |
| - | (<a href="http://parts.igem.org/Part:BBa_K733013">K733013</a>),
| |
| - | the open reading frame of EcAMP-1 and
| |
| - | a double terminator (<a href="http://parts.igem.org/Part:BBa_B0015">B0015</a>).<br>
| |
| - | The EcAMP-1 part was codon optimized for <i>E. coli</i> by the iGEM Utah State team
| |
| - | and thanks to the Life Technologies GeneArt software program. </p>
| |
| - | <img src="https://static.igem.org/mediawiki/2014/7/74/BBa_K1364011.png">
| |
| - | <p class="title4">Type</p>
| |
| - | <p class="texte">Generator</p>
| |
| - | <p class="title4">Tests</p>
| |
| - | <p class="texte">This part is not tested yet.</p>
| |
| - | <p class="title4">References</p>
| |
| - | <p class="texte"><!--à compléter : demander à emeline--></p>
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <p class="title3">Fungicide operons</p>
| |
| - |
| |
| - | <!--K1364013 : Pveg + RBS + GAFP + RBS + D4E1 + Terminator-->
| |
| - | <p class="title3" id="select11">
| |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364013">BBa_K1364013</a>: P<sub>veg</sub> - SpoVG - Antifungal EcAMP-1 - Double terminator</p>
| |
| - | <p class="texte">
| |
| - | <p class="title4">Design</p>
| |
| - | <p class="texte">This part is designed for the co-expression of two different peptides
| |
| - | with anti-fungal activities : D4E1 and GAFP-1.
| |
| - | It is composed of the strong, constitutive promotor of <i>B. subtilis</i>
| |
| - | P<sub>veg</sub> (<a href="http://parts.igem.org/wiki/index.php/Part:BBa_K823003">K823003</a>)
| |
| - | and the translation unit with GAFP-1 and D4E1 (BBa_K1364012).</p>
| |
| - | <img src="https://static.igem.org/mediawiki/2014/4/40/BBa_K1364013.png">
| |
| - | <p class="title4">Type</p>
| |
| - | <p class="texte">Generator</p>
| |
| - | <p class="title4">Tests</p>
| |
| - | <p class="texte">This part was tested on the fungi <i>Trichoderma reesei</i> (See <a href="https://2014.igem.org/Team:Toulouse/Result/experimental-results">Fungicides module</a>)</p>
| |
| - | <p class="title4">References</p>
| |
| - | See <a href="http://parts.igem.org/Part:BBa_K1364002">BBa_K1364002</a> and <a href="http://parts.igem.org/Part:BBa_K1364003">BBa_K1364003</a>.</p>
| |
| - | <br>
| |
| - | <p class="title2">Basic tools</p>
| |
| - |
| |
| - | <!--K1364015-->
| |
| - | <p class="title3" id="select12">
| |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364015">BBa_K1364015</a>: P<sub>veg</sub> + RFP</p>
| |
| - | <p class="texte"><!--Compléter-->
| |
| - | </p>
| |
| - | <p class="title4">Design</p>
| |
| - | <p class="texte">Coming soon! </p>
| |
| - | <p class="title4">Type</p>
| |
| - | <p class="texte">Reporter</p>
| |
| - | <p class="title4">Tests</p>
| |
| - | <p class="texte">This part was not tested yet</p>
| |
| - | <p class="title4">References</p>
| |
| - | <p class="texte">Coming soon!</p>
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <!--K1364016-->
| |
| - | <p class="title3" id="select13">
| |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364016">BBa_K1364016</a>: P<sub>lepA</sub> + RFP</p>
| |
| - | <p class="texte"><!--Compléter-->
| |
| - | </p>
| |
| - | <p class="title4">Design</p>
| |
| - | <p class="texte">Coming soon! </p>
| |
| - | <p class="title4">Type</p>
| |
| - | <p class="texte">Reporter</p>
| |
| - | <p class="title4">Tests</p>
| |
| - | <p class="texte">This part was not tested yet</p>
| |
| - | <p class="title4">References</p>
| |
| - | <p class="texte">Coming soon!</p>
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <!--K1364017-->
| |
| - | <p class="title3" id="select14">
| |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364017">BBa_K1364017</a>: P<sub>lepA</sub> + RBS SpoVG</p>
| |
| - | <p class="texte"><!--Compléter-->
| |
| - | </p>
| |
| - | <p class="title4">Design</p>
| |
| - | <p class="texte">Coming soon! </p>
| |
| - | <p class="title4">Type</p>
| |
| - | <p class="texte">Composite</p>
| |
| - | <p class="title4">Tests</p>
| |
| - | <p class="texte">This part was not tested yet</p>
| |
| - | <p class="title4">References</p>
| |
| - | <p class="texte">Coming soon!</p>
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <!--K1364019-->
| |
| - | <p class="title3" id="select15">
| |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364019">BBa_K1364019</a>: P<sub>veg</sub> + RBS + Antifungal EcAMP + Double terminator</p>
| |
| - | <p class="texte"><!--Compléter-->
| |
| - | </p>
| |
| - | <p class="title4">Design</p>
| |
| - | <p class="texte">Coming soon! </p>
| |
| - | <p class="title4">Type</p>
| |
| - | <p class="texte">Composite</p>
| |
| - | <p class="title4">Tests</p>
| |
| - | <p class="texte">This part was not tested yet</p>
| |
| - | <p class="title4">References</p>
| |
| - | <p class="texte">Coming soon!</p>
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <!--K1364021-->
| |
| - | <p class="title3" id="select16">
| |
| - | - <a href="http://parts.igem.org/Part:BBa_K1364021">BBa_K1364021</a>: Integrative plasmid for <i>Bacillus subtilis</i></p>
| |
| - | <p class="texte"><!--Compléter-->
| |
| - | </p>
| |
| - | <p class="title4">Design</p>
| |
| - | <p class="texte">Coming soon! </p>
| |
| - | <p class="title4">Type</p>
| |
| - | <p class="texte">Composite</p>
| |
| - | <p class="title4">Tests</p>
| |
| - | <p class="texte">This part was not tested yet</p>
| |
| - | <p class="title4">References</p>
| |
| - | <p class="texte">Coming soon!</p>
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <!--SOUS-TITRE-->
| |
| - | <p class="title1">Used parts</p>
| |
| - |
| |
| - | <br>
| |
| - |
| |
| - | <!--Table-->
| |
| - | <p class="texte"><center>
| |
| - | <table border="1" width="100%" height="20%">
| |
| - | <!--1ere ligne-->
| |
| - | <tr><td><p class="texte"><b>BioBrick</b></p></td>
| |
| - | <td><p class="texte"><b>Description</b></p></td>
| |
| - | <td><p class="texte"><b>Origine</b></p></td>
| |
| - | <td><p class="texte"><b>Module(s)</b></p></td></tr>
| |
| - | <!--2eme ligne-->
| |
| - | <tr><td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K733013">K733013</a></p></td>
| |
| - | <td><p class="texte">P<sub>veg</sub> (<a href="http://parts.igem.org/Part:BBa_K316001">K316001</a>) + RBS-SpoVG (<a href="http://parts.igem.org/Part:BBa_K143021">K143021</a>) : Constitutive promotor P<sub>veg</sub> and strong RBS for <i>B. subtilis</i></p></td>
| |
| - | <td><p class="texte">2014 Kit plate 1, 7H</p></td>
| |
| - | <td><p class="texte">Fungicides (EcAMP)</p></td>
| |
| - | </tr>
| |
| - | <!--3eme ligne-->
| |
| - | <tr>
| |
| - | <td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K823003">K823003</a></p></td>
| |
| - | <td><p class="texte">P<sub>veg</sub> : strong, constitutive promotor of <i>B. subtilis</i></p></td>
| |
| - | <td><p class="texte">2014 Kit plate 1, 20G</p></td>
| |
| - | <td><p class="texte">Fungicides (D4E1 GAFP-1)<br>Chemotaxis<br>Binding</p></td>
| |
| - | </tr>
| |
| - | <!--4eme ligne-->
| |
| - | <tr>
| |
| - | <td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K823002">K823002</a></p></td>
| |
| - | <td><p class="texte">P<sub>lepA</sub> : constitutive promotor of <i>B. subtilis</i>.
| |
| - | <br>It is the promotor of the lepA gene of <i>B. subtilis</i></p></td>
| |
| - | <td><p class="texte">2014 Kit plate 1, 20E</p></td>
| |
| - | <td><p class="texte">Fungicides (D4E1)<br>Chemotaxis<br>Binding</p></td>
| |
| - | </tr>
| |
| - | <!--5eme ligne-->
| |
| - | <tr>
| |
| - | <td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K606061">K606061</a></p></td>
| |
| - | <td><p class="texte">SpoVG RBS for <i>B. subtilis</i>
| |
| - | <br>Strong ribosome binding site from subtilis. Works fine in <i>E. coli</i></p></td>
| |
| - | <td><p class="texte">2014 Kit plate 1, 15A</p></td>
| |
| - | <td><p class="texte">Optional</p></td>
| |
| - | </tr>
| |
| - | <!--6eme ligne-->
| |
| - | <tr>
| |
| - | <td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K316027">K316027</a></p></td>
| |
| - | <td><p class="texte"><i>B. subtilis</i> transformation vector with LacI, targets amyE locus.</p></td>
| |
| - | <td><p class="texte">2014 Kit plate 1, 2N</p></td>
| |
| - | <td><p class="texte">Fungicides<br>Chemotaxis<br>Binding</p></td>
| |
| - | </tr>
| |
| - | <!--7eme ligne-->
| |
| - | <tr>
| |
| - | <td><p class="texte"><a href="http://parts.igem.org/Part:BBa_B0015">B0015</a></p></td>
| |
| - | <td><p class="texte">Double terminator (<a href="http://parts.igem.org/Part:BBa_B0010">BBa_B0010</a> + <a href="http://parts.igem.org/Part:BBa_B0012">BBa_B0012</a>)</p></td>
| |
| - | <td><p class="texte">2014 Kit plate 3, 3F</p></td>
| |
| - | <td><p class="texte">Fungicides (GAFP-1, EcAMP-1)</p></td>
| |
| - | </tr>
| |
| - | <!--8eme ligne-->
| |
| - | <tr>
| |
| - | <td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K780000">K780000</a></p></td>
| |
| - | <td><p class="texte">Terminator for <i>B. subtilis</i></p></td>
| |
| - | <td><p class="texte">Sent by the Warsaw iGEM team</p></td>
| |
| - | <td></td>
| |
| - | </tr>
| |
| - | <!--9eme ligne-->
| |
| - | <tr>
| |
| - | <td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K780001">K780001</a></p></td>
| |
| - | <td><p class="texte">Strong RBS for <i>B. subtilis</i></p></td>
| |
| - | <td><p class="texte">Sent by the Warsaw iGEM team</p></td>
| |
| - | <td></td>
| |
| - | </tr>
| |
| - | <!--10eme ligne-->
| |
| - | <tr>
| |
| - | <td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K780002">K780002</a></p></td>
| |
| - | <td><p class="texte">Strong RBS for <i>B. subtilis</i></p></td>
| |
| - | <td><p class="texte">Sent by the Warsaw iGEM team</p></td>
| |
| - | <td></td>
| |
| - | </tr>
| |
| - | <!--11eme ligne-->
| |
| - | <tr>
| |
| - | <td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K780003">K780003</a></p></td>
| |
| - | <td><p class="texte">Strong constitutive promotor for <i>B. subtilis</i></p></td>
| |
| - | <td><p class="texte">Sent by the Warsaw iGEM team</p></td>
| |
| - | <td><p class="texte">Fungicides<br>Chemotaxis<br>Binding</p></td>
| |
| - | </tr>
| |
| - | <!--12eme ligne-->
| |
| - | <tr>
| |
| - | <td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K1162001">K1162001</a></p></td>
| |
| - | <td><p class="texte">EcAMP-1</p></td>
| |
| - | <td><p class="texte">Sent by the Utah State iGEM team</p></td>
| |
| - | <td><p class="texte">Fungicides (EcAMP-1)</p></td>
| |
| - | </tr>
| |
| - | <!--13eme ligne-->
| |
| - | <tr>
| |
| - | <td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K823023">K823023</a></p></td>
| |
| - | <td><p class="texte">Empty backbone vector pSB<sub>BS</sub>1C for integration into <i>B. subtilis</i>
| |
| - | <br>It integrates in the amyE locus</p></td>
| |
| - | <td><p class="texte">Sent by the Munich iGEM team</p></td>
| |
| - | <td><p class="texte">Fungicides<br>Chemotaxis<br>Binding</p></td></tr>
| |
| - | <!--14eme ligne-->
| |
| - | <tr>
| |
| - | <td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K823022">K823022</a></p></td>
| |
| - | <td><p class="texte">Empty backbone vector pSB<sub>BS</sub>4S for integration into<i>B. subtilis</i>
| |
| - | <br>It integrates in the thrC locus</p></td>
| |
| - | <td><p class="texte">Sent by the Munich iGEM team</p></td>
| |
| - | <td><p class="texte">Fungicides<br>Chemotaxis<br>Binding</p></td>
| |
| - | </tr>
| |
| - | <!--15eme ligne-->
| |
| - | <tr>
| |
| - | <td><p class="texte"><a href="http://parts.igem.org/Part:BBa_K823021">K823021</a></p></td>
| |
| - | <td><p class="texte">Reporter vector pSB<sub>BS</sub>1C-lacZ for <i>B. subtilis</i></p></td>
| |
| - | <td><p class="texte">Sent by the Munich iGEM team</p></td>
| |
| - | <td><p class="texte">Fungicides<br>Chemotaxis<br>Binding</p></td></tr>
| |
| - | </table></center>
| |
| - | </p>
| |
| - |
| |
| - | <br>
| |
| | | | |
| | </div> | | </div> |
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