Team:ETH Zurich/blog

From 2014.igem.org

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<h3><a href="#">Week 1 : 9-15 June</a></h3>
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We did .........
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<h4>Tuesday, June 10th</h4>
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Preparation of
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-SOC
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-TFB1
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-TFB2 (pH was set to 5.6 instead of 5.8)
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-Agar plates (containing ampicillin (200 mg/L) or chloramphenicol (34 mg/L))
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According to qiagen DNA protocols & application
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Modifications:
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-150 ml Glycerol per 1 L (15%) are added to TFB1 and TFB2 (instead of 15 mL)
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-1.4 g/l CaCl2 dihydrate are used in TFB1
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-11.0 g/l CaCl2 dihydrate are used in TFB2
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-SOC is sterilized by filtration (not autoclaved)
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Revision as of 12:54, 2 July 2014

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Magna laoreet et aliquam

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