Team:Hannover/Results/Heavy Metals/Arabidopsis

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<h2>Results</h2>
<h2>Results</h2>
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<p class="text">We achieved to regenerate young <i>A. thaliana</i> after the transformation with our T4MBP.</p>
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<p class="text">The exchange of the promoter was checked by sequencing. We achieved to regenerate young <i>A. thaliana</i> after the transformation with our T4MBP.</p>
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<center><table border="0"><tr><td><a href="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141012_Arabudopsis-results1.jpg
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<center><table border="0"><tr><td><a href="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141012_Arabudopsis-results1.jpg" data-lightbox="galery1" data-title="Fig. 1a: Young potentially transgenic <i>A. thaliana</i> during the transfer from medium to earth. "><img src="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141012_Arabudopsis-results1.jpg" width="300px"></a></td><td><a href="https://static.igem.org/mediawiki/2014/1/13/Hannover_20141012_Arabudopsis-results3.jpg
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" data-lightbox="galery1" data-title="Fig. 1a: Young potentially transgenic <i>A. thaliana</i> during the transfer from medium to earth. "><img src="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141012_Arabudopsis-results1.jpg" width="300px"></a></td><td><a href="https://static.igem.org/mediawiki/2014/1/13/Hannover_20141012_Arabudopsis-results3.jpg
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" data-lightbox="galery1" data-title="Fig. 1b: Young potentially transgenic <i>A. thaliana</i> in medium."><img src="https://static.igem.org/mediawiki/2014/1/13/Hannover_20141012_Arabudopsis-results3.jpg" width="330px"></a></td><td><a href="https://static.igem.org/mediawiki/2014/3/35/Hannover_20141012_Arabudopsis-results2.jpg
" data-lightbox="galery1" data-title="Fig. 1b: Young potentially transgenic <i>A. thaliana</i> in medium."><img src="https://static.igem.org/mediawiki/2014/1/13/Hannover_20141012_Arabudopsis-results3.jpg" width="330px"></a></td><td><a href="https://static.igem.org/mediawiki/2014/3/35/Hannover_20141012_Arabudopsis-results2.jpg
" data-lightbox="galery1" data-title="Fig. 1c:Young potentially transgenic <i>A. thaliana</i> in earth."><img src="https://static.igem.org/mediawiki/2014/3/35/Hannover_20141012_Arabudopsis-results2.jpg" width="330px"></a></td>
" data-lightbox="galery1" data-title="Fig. 1c:Young potentially transgenic <i>A. thaliana</i> in earth."><img src="https://static.igem.org/mediawiki/2014/3/35/Hannover_20141012_Arabudopsis-results2.jpg" width="330px"></a></td>
</tr><tr><th colspan="3"><p class="text">Fig. 1: Pictures showing young potentially transgenic <i>A. thaliana</i> before, during and after the transfer from medium to earth. </th></p></td></tr></table></center>
</tr><tr><th colspan="3"><p class="text">Fig. 1: Pictures showing young potentially transgenic <i>A. thaliana</i> before, during and after the transfer from medium to earth. </th></p></td></tr></table></center>
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<center><table border="0">
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<tr><td><a href="https://static.igem.org/mediawiki/2014/9/97/Hannover_20141015_PORE_E3_2x35S_T4MBP_Map-1.png" data-lightbox="galery1" data-title="Fig. 2a: pORE-E3 vector with 2x35S promoter"><img src="https://static.igem.org/mediawiki/2014/9/97/Hannover_20141015_PORE_E3_2x35S_T4MBP_Map-1.png" width="300px"></a></td>
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<td><a href="https://static.igem.org/mediawiki/2014/d/d1/Hannover_20141015_PORE_E3_2x35S%2BInsert_Anke_Fabian.dna_History.png" data-lightbox="galery1" data-title="Fig. 2b: detailed history of exchanging the enTCUP2 promoter with the 2x35S promoter."><img src="https://static.igem.org/mediawiki/2014/d/d1/Hannover_20141015_PORE_E3_2x35S%2BInsert_Anke_Fabian.dna_History.png" width="300px"></td></tr>
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<tr><th colspan="3"><p class="text">Fig. 2: Pictures showing the vector pORE_E3 with 2x35S promoter and the way of exchanging the enTCUP2 promoter with the 2x35S promoter. </th></p></td></tr></table></center>
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<p id="Fusszeile"><br><br><br><br><img src="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141010Fusszeile2.jpg" width="980px" style="float:right" /></p>
<p id="Fusszeile"><br><br><br><br><img src="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141010Fusszeile2.jpg" width="980px" style="float:right" /></p>
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Revision as of 15:53, 15 October 2014

Results / Heavy metals / Arabidopsis thaliana T4MBP

Labwork

Transformation of A. thaliana with our T4MBP:

  1. Exchange the original promoter enTCUP2 of the binary vector pORE_E3 (AY562536.1) into a 2x35s promoter using this protocol.
  2. We integrated our T4MBP in our modified pORE_E3_2x35S.
  3. Application of our floral dip method.
  4. After 6 weeks we harvested transgenic seeds from A. thaliana and plated these seeds on selection MSO-media.
  5. At last we potted transformed plants.

Results

The exchange of the promoter was checked by sequencing. We achieved to regenerate young A. thaliana after the transformation with our T4MBP.

Fig. 1: Pictures showing young potentially transgenic A. thaliana before, during and after the transfer from medium to earth.

Fig. 2: Pictures showing the vector pORE_E3 with 2x35S promoter and the way of exchanging the enTCUP2 promoter with the 2x35S promoter.





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