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Team:Hannover/Protocols/Detection/Confocal
From 2014.igem.org
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| - | <h1><a href="https://2014.igem.org/Team:Hannover/Protocols" target="_blank">Protocols</a> / | + | <h1><a href="https://2014.igem.org/Team:Hannover/Protocols" target="_blank">Protocols</a> / Confocal Microscopy</h1> |
<table colspan="2"><tr><td><h4>Material:</h4></td> | <table colspan="2"><tr><td><h4>Material:</h4></td> | ||
Revision as of 15:51, 15 October 2014
Protocols / Confocal Microscopy
Material: |
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| Prepared MS Samples | |
| Confocal Microscope | (here:Eclipse TE2000-E, Nikon, Japan) Settings
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Before samples are screened by powerful lasers, which could bleach the GFP signal, the mercury based transmission light might be used for browsing the sample. After the right sample layer was focused, the source of lightening was switched to the lasers and the pinhole value needed to be adjusted. Three pictures were taken: one in the green channel, one in the red channel and one of in pseudo-transmission light. GFP molecules gave a green signal, while the chlorophyll visualized the chloroplasts as red dots. In order to compare the pictures, a scale bar was added in the pictures.
