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Team:Peking/secondtry/Parts
From 2014.igem.org
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<p>BBa_K1378009</p> | <p>BBa_K1378009</p> | ||
<p>Parts for Degradation</p> | <p>Parts for Degradation</p> | ||
| - | <p>BBa_K1378001</p> | + | <p>BBa_K1378001 is the coding sequence of protease MlrA which can specifically cleavage Microcystin(MC).</p> |
| - | <p>BBa_K1378022</p> | + | <p>BBa_K1378022 is the coding sequence of MlrA fused with PelB secretion tag.</p> |
| - | <p>BBa_K1378023</p> | + | <p>BBa_K1378023 is the coding sequence of MlrA fused with TorA secretion tag.</p> |
| - | <p>BBa_K1378024</p> | + | <p>BBa_K1378024 is the coding sequence of MlrA with terminator.</p> |
| - | <p>BBa_K1378025</p> | + | <p>BBa_K1378025 is the coding sequence of PelB-MlrA fusion protein with terminator.</p> |
| - | <p>BBa_K1378026</p> | + | <p>BBa_K1378026 is the coding sequence of TorA-MlrA fusion protein with terminator.</p> |
<p>Parts for Suicide</p> | <p>Parts for Suicide</p> | ||
<p>BBa_K1378031 is the coding sequence of cytoplasmic membrane protein Holin.</p> | <p>BBa_K1378031 is the coding sequence of cytoplasmic membrane protein Holin.</p> | ||
Revision as of 15:12, 15 October 2014
Part
Favorite Parts
INPNC-MVN-mRFP
Main Page-BBa_K1378005
BBa_K1378005 is a composite part coding INPNC-MVN-mRFP fusion protein. We confirmed that fusion protein is truly displayed outwards by using the INPNC displaying system by rTEV protease digestion assay method and thus help characterize INPNC part.
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MlrA
Main Page-BBa_K1378001
MlrA is a protease which can cleavage Microcystin(MC) and and thus reduce its toxicity. We tested it in E.coli and showed that MlrA can decrease the concentration of MC and weaken its inhibitory activity to Protein Phosphatase 1.
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Part List
Parts for Killing Improvement
BBa_K1378003 is the coding sequence of lectin Microvirin.
BBa_K1378005
BBa_K1378007
BBa_K1378009
Parts for Degradation
BBa_K1378001 is the coding sequence of protease MlrA which can specifically cleavage Microcystin(MC).
BBa_K1378022 is the coding sequence of MlrA fused with PelB secretion tag.
BBa_K1378023 is the coding sequence of MlrA fused with TorA secretion tag.
BBa_K1378024 is the coding sequence of MlrA with terminator.
BBa_K1378025 is the coding sequence of PelB-MlrA fusion protein with terminator.
BBa_K1378026 is the coding sequence of TorA-MlrA fusion protein with terminator.
Parts for Suicide
BBa_K1378031 is the coding sequence of cytoplasmic membrane protein Holin.
BBa_K1378032 is the coding sequence of peptidoglycan hydrolase Endolysin.
