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Team:HIT-Harbin/Design
From 2014.igem.org
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<p> We combined yellow fluorescent protein induced by dioxins mentioned above together with DNA binding auxiliary sequence of lexAop and mdr521-805. Through the transformation of this genetic sequence, our device can express the yellow fluorescent protein massively and rapidly if the result of dioxin moleculesd detection is positive. In addition, as a result of the positive feedback effect, after the dioxin is removed, the device can still express the yellow fluorescent protein steadily, achieving the function of signal enhancement and memorization. In the absence of ligand, AhR is present in the cytosol in a complex with Hsp90, XAP2 and p23 proteins. Upon binding to a ligand, the AhR complex translocates into the nucleus and the AhR dissociates from Hsp90 complex to form a heterodimer with its partner molecule, Arnt. Thus, the formed AhR/Arnt heterodimer recognizes an enhancer DNA element designated xenobiotic responsive element (XRE) sequence located in the promoter region of CYP1A1gene, resulting in the enhanced expression of the gene[1].</p> | <p> We combined yellow fluorescent protein induced by dioxins mentioned above together with DNA binding auxiliary sequence of lexAop and mdr521-805. Through the transformation of this genetic sequence, our device can express the yellow fluorescent protein massively and rapidly if the result of dioxin moleculesd detection is positive. In addition, as a result of the positive feedback effect, after the dioxin is removed, the device can still express the yellow fluorescent protein steadily, achieving the function of signal enhancement and memorization. In the absence of ligand, AhR is present in the cytosol in a complex with Hsp90, XAP2 and p23 proteins. Upon binding to a ligand, the AhR complex translocates into the nucleus and the AhR dissociates from Hsp90 complex to form a heterodimer with its partner molecule, Arnt. Thus, the formed AhR/Arnt heterodimer recognizes an enhancer DNA element designated xenobiotic responsive element (XRE) sequence located in the promoter region of CYP1A1gene, resulting in the enhanced expression of the gene[1].</p> | ||
| - | <img id="Family" width=" | + | <img id="Family" width="450px" src="https://static.igem.org/mediawiki/2014/7/75/Design1.png"> |
<p>Reference：[1] Functional role of AhR in the expression of toxic effects by TCDD</p> | <p>Reference：[1] Functional role of AhR in the expression of toxic effects by TCDD</p> | ||
Revision as of 14:24, 15 October 2014
Design
DIOXIN DETECTIVE
DIOXIN SENSOR
AhR RECEPTOR
We combined yellow fluorescent protein induced by dioxins mentioned above together with DNA binding auxiliary sequence of lexAop and mdr521-805. Through the transformation of this genetic sequence, our device can express the yellow fluorescent protein massively and rapidly if the result of dioxin moleculesd detection is positive. In addition, as a result of the positive feedback effect, after the dioxin is removed, the device can still express the yellow fluorescent protein steadily, achieving the function of signal enhancement and memorization. In the absence of ligand, AhR is present in the cytosol in a complex with Hsp90, XAP2 and p23 proteins. Upon binding to a ligand, the AhR complex translocates into the nucleus and the AhR dissociates from Hsp90 complex to form a heterodimer with its partner molecule, Arnt. Thus, the formed AhR/Arnt heterodimer recognizes an enhancer DNA element designated xenobiotic responsive element (XRE) sequence located in the promoter region of CYP1A1gene, resulting in the enhanced expression of the gene[1].
Reference：[1] Functional role of AhR in the expression of toxic effects by TCDD
lexA DBD/Mdr
We combined yellow fluorescent protein induced by dioxins mentioned above together with DNA binding auxiliary sequence of lexAop and mdr521-805. Through the transformation of this genetic sequence, our device can express the yellow fluorescent protein massively and rapidly if the result of dioxin moleculesd detection is positive. In addition, as a result of the positive feedback effect, after the dioxin is removed, the device can still express the yellow fluorescent protein steadily, achieving the function of signal enhancement and memorization.
MEMORY SYSTEM
Here we add a rational design of cellular memory in yeast that employs autoregulatory transcriptional positive feedback .We combined yellow fluorescent protein induced by dioxins mentioned above together with DNA binding auxiliary sequence of lexAop and mdr521-805. Through the transformation of this genetic sequence, our device can express the yellow fluorescent protein massively and rapidly if the result of dioxin moleculesd detection is positive. In addition, as a result of the positive feedback effect, after the dioxin is removed, the device can still express the yellow fluorescent protein steadily, achieving the function of signal enhancement and memorization.
DIOXIN DEGRADEE
DIOXIN CONCENTRATION
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