Team:KAIT Japan/Notebook

From 2014.igem.org

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:<big>'''Date:9/4'''</big> Restriction enzyme treatment the HlyA and GFP
:<big>'''Date:9/4'''</big> Restriction enzyme treatment the HlyA and GFP
:<big>'''Date:9/5'''</big> Restriction enzyme treatment the vector
:<big>'''Date:9/5'''</big> Restriction enzyme treatment the vector
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:<big>'''Date:9/5'''</big> Ligation the vector and unsere
:<big>'''Date:9/6'''</big>
:<big>'''Date:9/6'''</big>
:<big>'''Date:9/7'''</big>
:<big>'''Date:9/7'''</big>

Revision as of 09:43, 15 October 2014

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KAIT Japan2013 Kanako.png

KAIT Japan 2014 iGEMRogo.png
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Home

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Team

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Project

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Parts

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Protocol

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Notebook

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Results

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Safety

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Human Practice


Notebook

We made an experiment every day. It was process at trial and error.



Creating parts of HlyA and GFP

Date:8/22 The refinement of DNA and PCR and Electrophoresis
Date:8/25 PCR and Electrophoresis and Restriction
Date:8/26 Insert gene into TAvectar and Transformation
Date:8/26 Blue white Selection and Electrophoresis(8/25 Restriction) and DNA Extraction
Date:8/27 Electrophoresed the DNA that We did refinement on August 26 to confirmed it
Date:8/28 Check the HlyA and GFP for colony PCR
Date:8/29 DNA purify the HlyA and GFP
Date:8/29 Ligation product and check for PCR
Date:9/1 Build replica the HlyA and GFP
Date:9/1 Restriction enzyme treatment the HlyA and GFP
Date:9/2 Restriction enzyme treatment the HlyA and GFP in 9/1 is NANOSEP
Date:9/3 Ligation and PCR, DNA purification the HlyA and GFP
Date:9/4 Restriction enzyme treatment the HlyA and GFP
Date:9/5 Restriction enzyme treatment the vector
Date:9/5 Ligation the vector and unsere
Date:9/6
Date:9/7
Date:9/8
Date:9/9 Check the HiyA and GFP replica.Ligation and Transformation the vector and insert DNA.
Date:9/10 Transformation in 9/9 check the colony PCR.TA cloning the HlyA+GFP.
Date:9/11 Ligation the vector and insert DNA.
Date:9/12~9/15 Transformation the Ligation product.
Date:9/16 Noticed wrong the primer.so restart from the First.
Date:9/17 PCR the DNA abstracted for iGEM kit.It was TA cloning and restriction enzyme treatment.
Date:9/18 Ligation the GFP and HlyA.
Date:9/19
Date:9/20
Date:9/21
Date:9/22
Date:9/23
Date:9/24
Date:9/25
Date:9/26
Date:9/27
Date:9/28
Date:9/29
Date:9/30
Date:10/1
Date:10/2
Date:10/3
Date:10/4
Date:10/5
Date:10/6




Creating parts of STAT3

Date:8/19 PCR(8/18 Miniprep) and Electrophoresis
Date:8/20~8/22 PCR(Lowered 2℃ from Tm value.) and Electrophoresis
Date:8/25 PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)
Date:8/26 PCR and Electrophoresis(Using a mutation primer.)
Date:8/27 First Variation introduction (with Prime STAR Max)
Date:9/1
Date:9/2 PCR and Electrophoresis
Date:9/3
Date:9/5~9/8 PCR and Electrophoresis
Date:9/9 Sequence(to To confirm whether variation happened in DNA of STAT3 )
Date:9/10~9/12 PCR and Electrophoresis
Date:9/13
Date:9/14
Date:9/15
Date:9/16
Date:9/17
Date:9/18
Date:9/19
Date:9/20
Date:9/21
Date:9/22
Date:9/23
Date:9/24
Date:9/25
Date:9/26
Date:9/27
Date:9/28
Date:9/29
Date:9/30
Date:10/1
Date:10/2
Date:10/3
Date:10/4
Date:10/5
Date:10/6




Creating parts of IL-10α,IL-10β

Date:8/19 PCR(8/18 Miniprep) and Electrophoresis
Date:8/20~8/22 PCR(Lowered 2℃ from Tm value.) and Electrophoresis
Date:8/25 PCR and Electrophoresis(Changed to Taq HOTSTER from EX taq.)
Date:8/26 PCR and Electrophoresis(Using a mutation primer.)
Date:8/27 First Variation introduction (with Prime STAR Max)
Date:9/1
Date:9/2
Date:9/3
Date:9/4
Date:9/5
Date:9/6
Date:9/7
Date:9/8
Date:9/9 Sequence(to To confirm whether variation happened in DNA of IL-10α,IL-10β)
Date:9/10-10/9 Sequence preparation & Sequence


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