Team:Hannover/Results/Heavy Metals/Arabidopsis

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<h2>Labwork</h2><p class="text"><ul><li>floral dip method using<a href="" target="_blank"> our protocol</a></li><ol><li>integrated our T4MBP in our modified pORE_E3_2x35S</li><li>floral dip</li><li>after 6 weeks we harvested transgenic seeds from <i>A. thaliana</i> and plated this seeds on selection MSO-media</li><li>potted transformed plants</li></ol></ul></p>
<h2>Labwork</h2><p class="text"><ul><li>floral dip method using<a href="" target="_blank"> our protocol</a></li><ol><li>integrated our T4MBP in our modified pORE_E3_2x35S</li><li>floral dip</li><li>after 6 weeks we harvested transgenic seeds from <i>A. thaliana</i> and plated this seeds on selection MSO-media</li><li>potted transformed plants</li></ol></ul></p>
<h2>Results</h2>
<h2>Results</h2>
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<p class="text">We achieved to regenerated young <i>A. thaliana</i> after the transformation them with our T4MBP.
<center><table border="0"><tr><td><a href="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141012_Arabudopsis-results1.jpg
<center><table border="0"><tr><td><a href="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141012_Arabudopsis-results1.jpg
" data-lightbox="galery1" data-title="Fig. 1a: Young potentially transgenic <i>A. thaliana</i> during the transfer from medium to earth. "><img src="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141012_Arabudopsis-results1.jpg" width="300px"></a></td><td><a href="https://static.igem.org/mediawiki/2014/1/13/Hannover_20141012_Arabudopsis-results3.jpg
" data-lightbox="galery1" data-title="Fig. 1a: Young potentially transgenic <i>A. thaliana</i> during the transfer from medium to earth. "><img src="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141012_Arabudopsis-results1.jpg" width="300px"></a></td><td><a href="https://static.igem.org/mediawiki/2014/1/13/Hannover_20141012_Arabudopsis-results3.jpg

Revision as of 07:16, 15 October 2014

Results / Heavy metals / Arabidopsis thaliana T4MBP

Labwork

  • floral dip method using our protocol
    1. integrated our T4MBP in our modified pORE_E3_2x35S
    2. floral dip
    3. after 6 weeks we harvested transgenic seeds from A. thaliana and plated this seeds on selection MSO-media
    4. potted transformed plants

Results

We achieved to regenerated young A. thaliana after the transformation them with our T4MBP.

Fig. 1: Pictures showing young potentially transgenic A. thaliana before, during and after the transfer from medium to earth.





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