Team:Brasil-SP/Notebook/Interlab Study

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<p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;As part of the first international interlab measurement study in synthetic biology, all Measurement Track teams were required to obtain fluorescence data for three specific genetic devices. They are<p/>
<p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;As part of the first international interlab measurement study in synthetic biology, all Measurement Track teams were required to obtain fluorescence data for three specific genetic devices. They are<p/>

Revision as of 05:42, 15 October 2014

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Measurement Interlab Study


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Retrieved from "http://2014.igem.org/Team:Brasil-SP/Notebook/Interlab_Study"

    Protocols

     As part of the first international interlab measurement study in synthetic biology, all Measurement Track teams were required to obtain fluorescence data for three specific genetic devices. They are

Biobrick device 1: BBa_I20260, an existing device


Assembly Protocol Biobrick Device 1

Biobrick device 2: BBa_J23101 + BBa_E0240, a new device built by each team


Assembly Protocol Biobrick Device 2 and 3


Biobrick device 3: BBa_J23115 + BBa_E0240, a new device built by each team.
Note: Compared to biobrick device 1 and 2, biobrick device 3 was constructed with a different promoter (BBa_J23115).


Assembly Protocol Biobrick Device 2 and 3

      After colect and store data, it was necessary to analyze our results. Data file format standard for flow cytometry is FCS (Flow Cytometry Standard), it contains all information about data acquired, instrument used and sample. In order to extract useful data we converted .fcs file to .csv using GenePattern Flow Cytometry suite.

    Results

      For the proposed specific genetic devices, we have measured fluorescence by Flow Cytometry and Fluorometry. Device 1 and 2 are equivalent assemblies, however, we did not expect equal fluorescence values since device 1 was an existing device and 2 was built. When it comes to device 3, only the promoter was changed from BBa_J23101 to BBa_J23115, in all likelihood, differing in strength.


     


Flow cytometry - Mean values