Team:Brasil-SP/Notebook/Interlab Study

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<p><h2>Measurement Interlab Study</h2></p>
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<p><h2>Measurement Interlab Study</h2></p>
 
<p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;As part of the first international interlab measurement study in synthetic biology, all Measurement Track teams were required to obtain fluorescence data for three specific genetic devices. They are<p/>
<p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;As part of the first international interlab measurement study in synthetic biology, all Measurement Track teams were required to obtain fluorescence data for three specific genetic devices. They are<p/>
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<p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; For the proposed specific genetic devices, we have measured fluorescence by <strong>Flow Cytometry</strong> and <strong>Fluorometry</strong>. Device 1 and 2 are equivalent assemblies, however, we did not expect equal fluorescence values since device 1 was an existing device and 2 was built. When it comes to device 3, only the promoter was changed. </p>
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<p align="justify">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; For the proposed specific genetic devices, we have measured fluorescence by <strong>Flow Cytometry</strong> and <strong>Fluorometry</strong>. Device 1 and 2 are equivalent assemblies, however, we did not expect equal fluorescence values since device 1 was an existing device and 2 was built. When it comes to device 3, only the promoter was changed from BBa_J23101 to BBa_J23115, in all likelihood, differing in strength.</p>

Revision as of 04:04, 15 October 2014

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Measurement Interlab Study


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     As part of the first international interlab measurement study in synthetic biology, all Measurement Track teams were required to obtain fluorescence data for three specific genetic devices. They are

Biobrick device 1: BBa_I20260, an existing device


Assembly Protocol Biobrick Device 1

Biobrick device 2: BBa_J23101 + BBa_E0240, a new device built by each team


Assembly Protocol Biobrick Device 2 and 3


Biobrick device 3: BBa_J23115 + BBa_E0240, a new device built by each team.
Note: Compared to biobrick device 1 and 2, biobrick device 3 was constructed with a different promoter (BBa_J23115).


Assembly Protocol Biobrick Device 2 and 3

    Results

      For the proposed specific genetic devices, we have measured fluorescence by Flow Cytometry and Fluorometry. Device 1 and 2 are equivalent assemblies, however, we did not expect equal fluorescence values since device 1 was an existing device and 2 was built. When it comes to device 3, only the promoter was changed from BBa_J23101 to BBa_J23115, in all likelihood, differing in strength.


Flow cytometry - Mean values