Team:Austin Texas/interlab study
From 2014.igem.org
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===Sample Preparation=== | ===Sample Preparation=== | ||
- | *First, inoculate 10 mL of LB with frozen stocks of the three | + | *First, inoculate triplicate 10 mL cultures of LB with frozen stocks of the three devices (in TOP10 cells; I20260, J23101+E0240, J23115+E0240), a cell background control (TOP10 cells), and an LB only control in a 50 mL Erlenmeyer flask. Grow for 16-18 hours at 37°C, shaking at 300rpm. |
- | *The next morning, add 10µL of each overnight culture to 10 mL of LB with three replicates of each (15 total flasks) and grow 16-18 hours at 37°C, shaking at 300rpm. | + | *The next morning, add 10µL of each overnight culture to 10 mL of LB with three replicates of each (15 total flasks) and grow 16-18 hours at 37°C, shaking at 300rpm. Triplicate cultures were continued. |
*After 16-18 hours, add 80 µL of each culture to the wells of a clear-bottomed black 96-well plate. | *After 16-18 hours, add 80 µL of each culture to the wells of a clear-bottomed black 96-well plate. | ||
Revision as of 00:22, 15 October 2014
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