Team:Austin Texas/interlab study

From 2014.igem.org

(Difference between revisions)
(Measurement Protocol)
(Sample Preparation)
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===Sample Preparation===
===Sample Preparation===
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*First, inoculate 10 mL of LB with frozen stocks of the three transformed clones (I20260, J23101+E0240, J23115+E0240), a cell background control (TOP10 electrocompetent cells), and an LB only control in a 50 mL Erlenmeyer flask. Grow for 16-18 hours at 37°C, shaking at 300rpm.  
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*First, inoculate triplicate 10 mL cultures of LB with frozen stocks of the three devices (in TOP10 cells; I20260, J23101+E0240, J23115+E0240), a cell background control (TOP10 cells), and an LB only control in a 50 mL Erlenmeyer flask. Grow for 16-18 hours at 37°C, shaking at 300rpm.  
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*The next morning, add 10µL of each overnight culture to 10 mL of LB with three replicates of each (15 total flasks) and grow 16-18 hours at 37°C, shaking at 300rpm.
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*The next morning, add 10µL of each overnight culture to 10 mL of LB with three replicates of each (15 total flasks) and grow 16-18 hours at 37°C, shaking at 300rpm.  Triplicate cultures were continued.
*After 16-18 hours, add 80 µL of each culture to the wells of a clear-bottomed black 96-well plate.
*After 16-18 hours, add 80 µL of each culture to the wells of a clear-bottomed black 96-well plate.

Revision as of 00:22, 15 October 2014