Team:Tuebingen/Notebook/Protocols/PCR

From 2014.igem.org

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<h3>Polymerase chain reaction</h3>
<h3>Polymerase chain reaction</h3>
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<h4>Reagents</h4>
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<table border="0">
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  <colgroup>
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    <col width="100">
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    <col width="300">
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  </colgroup>
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  <tr>
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    <td style="text-align: center">&nbsp;</td>
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    <td>Template (10-100 ng)</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">1 µL</td>
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    <td>Primer forward (10 µM)</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">1 µL</td>
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    <td>Primer reverse (10 µM)</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">1 µL</td>
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    <td>dNTP mix (10 mM)</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">5 µL</td>
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    <td>10x PCR buffer</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">0.5 µL</td>
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    <td>Pfu Polymerase (1 U/µL)</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">to 50 µL</td>
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    <td>H20</td>
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  </tr>
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</table>
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 +
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<h4>Procedure</h4>
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<p>The reagents are mixed in a PCR-tube in which the polymerase is added last. Run the cycler
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 +
using the appropriate program for the used polymerases and primers.</p>

Revision as of 22:54, 14 October 2014


Polymerase chain reaction

Reagents

  Template (10-100 ng)
1 µL Primer forward (10 µM)
1 µL Primer reverse (10 µM)
1 µL dNTP mix (10 mM)
5 µL 10x PCR buffer
0.5 µL Pfu Polymerase (1 U/µL)
to 50 µL H20

Procedure

The reagents are mixed in a PCR-tube in which the polymerase is added last. Run the cycler using the appropriate program for the used polymerases and primers.

Retrieved from "http://2014.igem.org/Team:Tuebingen/Notebook/Protocols/PCR"