Team:Tuebingen/Notebook/Protocols/Ni NTA Buffers

From 2014.igem.org

(Difference between revisions)
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<p> Lysis buffer (pH = 8)
<p> Lysis buffer (pH = 8)
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<table border="0">
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  <colgroup>
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    <col width="100">
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    <col width="300">
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  </colgroup>
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 +
  <tr>
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    <td style="text-align: center">50 mM</td>
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    <td>NaH2PO4</td>
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  </tr>
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 +
  <tr>
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    <td style="text-align: center">300 mM</td>
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    <td>NaCl</td>
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  </tr>
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  <tr>
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    <td style="text-align: center">10 mM</td>
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    <td>Immidazol</td>
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  </tr>
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</table>
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<p>&nbsp;</p>
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<p> Wash buffer (pH = 8)
 +
<table border="0">
 +
  <colgroup>
 +
    <col width="100">
 +
    <col width="300">
 +
  </colgroup>
 +
 +
  <tr>
 +
    <td style="text-align: center">50 mM</td>
 +
    <td>NaH2PO4</td>
 +
  </tr>
 +
 +
  <tr>
 +
    <td style="text-align: center">300 mM</td>
 +
    <td>NaCl</td>
 +
  </tr>
 +
 +
  <tr>
 +
    <td style="text-align: center">20 mM</td>
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    <td>Immidazol</td>
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  </tr>
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</table>
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 +
<p>&nbsp;</p>
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 +
<p> Elution buffer (pH = 8)
<table border="0">
<table border="0">
   <colgroup>
   <colgroup>
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   </tr>
   </tr>
</table>
</table>
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</div>
</div>

Revision as of 22:48, 14 October 2014


Ni NA buffers

Lysis buffer (pH = 8)

50 mM NaH2PO4
300 mM NaCl
10 mM Immidazol

 

Wash buffer (pH = 8)

50 mM NaH2PO4
300 mM NaCl
20 mM Immidazol

 

Elution buffer (pH = 8)

50 mM NaH2PO4
300 mM NaCl
250 mM Immidazol

Retrieved from "http://2014.igem.org/Team:Tuebingen/Notebook/Protocols/Ni_NTA_Buffers"