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Team:Tuebingen/Notebook/Protocols/LB agar
From 2014.igem.org
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Revision as of 22:09, 14 October 2014
LB agar
Reagents
| 10 g | Bacto-tryptone |
| 5 g | yeast extract |
| 10 g | NaCl |
Procedure
- Add the regents to 800ml H2O
- Adjust pH to 7.5 with NaOH
- Add 15g agar
- Melt agar into solution in the microwave
- Adjust volume to 1L with dH2O
- Sterilize by autoclaving
