ATTRIBUTIONS

Team Students

Ted Baldwin and Cécile Piot participated in the characterization of the CpxR and split IFP stress-sensitive response.

Romane Breysse, Jin Chang and Bastien Duckert participated in the characterization of the osmo response with the HOG pathway in S. cerevisiae.

Arthur Giroux participated in the signal detection and processing with the Raspberry Pi. He also designed the wiki.

Nikolaus Huwiler participated in the CpxR dimerization characterization. He also improved an existing biobrick (RFP under CpxR promoter).

Sakura Nussbaum participated in the characterization of the full and split luciferases. She also organized the outreach event at EPFL.

Lucie Petetin participated in the characterization of the arabinose promoter and of the full and split luciferases. She also participated in the osmo response characterization.

Ione Pla participated in the characterization of the arabinose promoter. She also took care of submitting the biobricks to the registry, designing the metafluidics system, and of many administrative tasks.

Axel De Tonnac, Grégoire Repond and Thomas Simonet participated in the characterization of the luciferase. He also participated in the design and tests of the microfluidic chips and studied the safety of the chips.

The whole team worked together and contributed to each other's work.

Team Advisors

The TAs and Professors helped us with lab techniques and gave us a lot of feedback throughout the project.

External Help

Francesco Piraino, Henrike Niederholtmeyer and Francesca Volpetti helped us with autoCAD designing, microfluidic chips fabrication and general help during our experiments with microfluidic chips.

John McKinney and Jan van der Meer discussed the biosafety issues in microfluidics with us, which helped us in our Bio Safety part.

Viesturs Simanis and Andrea Krapp helped us a lot to begin the yeast part of the project. They clarified many points regarding yeast transformations and gene regulation in S. cerevisiae. They confirmed the feasibility of our project and validated the constructs we had designed.