Team:Tianjin/Safety

From 2014.igem.org

(Difference between revisions)
(Prototype team page)
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<br>On this page you can document your project, introduce your team members, document your progress <br> and share your iGEM experience with the rest of the world! </p>
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<br>
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<p style="color:#E7E7E7"> <a href="https://2014.igem.org/wiki/index.php?title=Team:Tianjin/Safety&action=edit"style="color:#FFFFFF"> Click here  to edit this page!</a> </p>
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<div class="fixed">
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      <table width="100%" border="0" cellpadding="0" cellspacing="5">
 +
        <tr bgcolor="#3A2108">
 +
          <td><span class="STYLE69"><a href="#part1">The safety of lab</a></span></td>
 +
        </tr>
 +
        <tr bgcolor="#3A2108">
 +
          <td><span class="STYLE69"><a href="#part2">The safety of synthetic biology</a></span></td>
 +
        </tr>
 +
        <tr bgcolor="#3A2108">
 +
          <td><span class="STYLE69"><a href="#part3">The safety of our project</a></span></td>
 +
        </tr>
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        <tr bgcolor="#3A2108">
 +
          <td height="35" bgcolor="#663300"><span class="STYLE69"><a href="#top">Go to the top</a></span></td>
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</div> 
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<table width="1230" border="0" cellspacing="0" cellpadding="0">
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  <tr>
 +
    <td width="19%" bgcolor="#3A2108"><p><a href="#https://2014.igem.org/Main_Page"><img src="../images/Team：Tianjin image IGEM_official_logo.png" alt="image igem" width="202" height="114" border="0" align="top" /></a><br />
 +
    </p>
 +
        <p>&nbsp;</p>
 +
      <p>&nbsp;</p>
 +
      <p>&nbsp;</p></td>
 +
    <td width="43%" bgcolor="#3A2108"><p><span class="STYLE43"><span class="STYLE24"><a name="top" id="top"></a><span class="STYLE77">T</span></span><span class="STYLE76">rans<em>f</em> ibre</span></span><br />
 +
            <span class="STYLE40">Welcome to Team Tianjin! </span><br />
 +
    </p></td>
 +
    <td width="38%" bgcolor="#3A2108"><img src="https://static.igem.org/mediawiki/2014/e/eb/Tianjin-head3.gif" width="456" height="226" align="right" /></td>
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  </tr>
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<table width="100%" border="0" cellspacing="0" cellpadding="0">
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  <tr>
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    <td width="11%" height="21"><div id="home">
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        <tr>
 +
          <td bordercolor="#311801"><span class="STYLE45"><a href="#https://2014.igem.org/Team:Tianjin">Home|</a></span></td>
 +
        </tr>
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        <table width="176" height="124" border="0" cellpadding="0" cellspacing="1">
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          <tr>
 +
            <td bgcolor="#666600"><span class="STYLE48"><a href="#">overview</a></span></td>
 +
          </tr>
 +
          <tr>
 +
            <td bgcolor="#666600"><a href="#" class="STYLE48">background</a></td>
 +
          </tr>
 +
          <tr>
 +
            <td bgcolor="#666600"><span class="STYLE48"><a href="#">design</a></span></td>
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          <tr>
 +
            <td bgcolor="#666600"><a href="#" class="STYLE48">results</a></td>
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          <tr>
 +
            <td bgcolor="#666600"><a href="#" class="STYLE48">future works </a></td>
 +
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 +
          <tr>
 +
            <td bgcolor="#666600"><a href="#" class="STYLE48">modeling</a></td>
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    <a href="#https://2014.igem.org/Team:Tianjin/Project" class="STYLE48">Project|</a></div></td>
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          <tr>
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          </tr>
 +
          <tr>
 +
            <td bgcolor="#666600"><a href="#" class="STYLE47">achievements</a></td>
 +
          </tr>
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        </table>
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      </div>
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      <table width="110" border="0" cellspacing="0" cellpadding="0">
 +
        <tr>
 +
          <td><a href="#https://2014.igem.org/Team:Tianjin/Judging" class="STYLE47">Judging|</a></td>
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          <tr>
 +
            <td bgcolor="#666600"><a href="#" class="STYLE47">methods</a></td>
 +
          </tr>
 +
          <tr>
 +
            <td bgcolor="#666600"><a href="#" class="STYLE47">labnotes</a></td>
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      <table width="110" border="0" cellspacing="0" cellpadding="0">
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        <tr>
 +
          <td><a href="#https://2014.igem.org/Team:Tianjin/Notebook" class="STYLE47">Notebook|</a></td>
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          <tr>
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            <td><span class="STYLE45"><a href="#https://2014.igem.org/Team:Tianjin/Team">Team|</a></span>
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                  <tr>
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 +
                  </tr>
 +
                  <tr>
 +
                    <td bgcolor="#666600"><a href="#" class="STYLE47">members</a></td>
 +
                  </tr>
 +
                  <tr>
 +
                    <td bgcolor="#666600"><span class="STYLE47"><a href="#">attribution</a></span></td>
 +
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 +
                  <tr>
 +
                    <td bgcolor="#666600"><a href="#" class="STYLE47">acknowledgment</a></td>
 +
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      <div id="layer2">
 +
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            <td><span class="STYLE45"><a href="#https://2014.igem.org/Team:Tianjin/Safety">Safety|</a></span>
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                      <td bgcolor="#666600"><a href="#" class="STYLE47">safety</a></td>
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                      <td bgcolor="#666600"><a href="#" class="STYLE47">ethics</a></td>
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            <td><span class="STYLE45"><a href="#https://2014.igem.org/Team:Tianjin/HumanPractice">Human&nbsp;Practice|</a></span>
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                    <td bgcolor="#666600"><span class="STYLE66"><a href="#">Weekly Newsletter</a></span></td>
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                    <td bgcolor="#666600" class="STYLE33"><span class="STYLE67"><a href="#" class="STYLE56"><strong>A Nationwide Questionnaire</strong></a></span></td>
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                    <td bgcolor="#666600"><strong class="STYLE33"><a href="#">Interaction Activities</a></strong></td>
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              <td bgcolor="#C67B14"><span class="STYLE62"><a name="part1" id="part1"></a><strong>①The safety of lab</strong></span></td>
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              <td height="2547" class="STYLE33"><span class="STYLE48">Alarm bells</span><br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;According to the Ministry of Education “2008-2009  General Undergraduate Colleges Laboratory Information Statistics”, there are  26479 laboratories in 1129 total regular undergraduate colleges in China,  laboratory area 2 6.015 million m2. With so many  laboratories, safety accidents have occured from time to time. In 2009, a  university teacher of the department of chemistry mistakenly accessed CO gas  into 307 lab, which should have been accessed to 211 lab. A woman doctor died  because of this fault; In 2010, students and teachers of a university in  northeast of China used goats without being checked in their experiments, which  lead 27 students and 1 teacher were diagnosed as infected with brucellosis; In  2013, &nbsp;a postgraduate student named Huang Yang from Fudan University died  of poisoning in Shanghai Zhongshan Hospital. According to the report of the  police's investigation, a toxic chemical compound was found in the drinking  water dispenser in Huang's dormitory room, and this toxic chemical compoud is  taken out of a laboratory.<br />
 +
              <img src="https://static.igem.org/mediawiki/2014/b/b4/Tianjin-safety.png" width="400" height="264" hspace="150" align="middle" /><br />
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              <p><span class="STYLE72"><strong>Ponder why</strong><br />
 +
                  <strong>——Why do such tragedies repeat again and again?</strong></span><br />
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;These terrible incidents are not mere  coincidences and it reminds us college students of the importance of  lab-safety. Thus we studied a large number of reports and literature to analyze  the causes of? laboratory accidents.  First of all, leaders and managers pay insufficient attention is the main  reason. Second, management system and mechanism are not sound. Lacking of  safety consciousness also cannot be neglected in causing safety problems. Last  but not least, non-standard hardware construction is also a key reason.<br />
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<span class="STYLE72"><span class="STYLE71"><strong>reflect on our own</strong><br />
 +
<strong>——What is happening to our lab?</strong></span></span><span class="STYLE71"><strong></strong></span><strong><br />
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</strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Laboratory  safety awareness is the &quot;Achilles heel&quot; to our lab. Too much facts  tell us that most accidents are caused by negligence of researchers. Do  experiments without wearing gloves or lab coats, pile up clutter at random in  the laboratory, or eat pineapples in the laboratory during experimental  clearances. Now ask yourself, have these phenomenon happened to you or around  you on earth before?<br />
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<img src="https://static.igem.org/mediawiki/2014/1/15/Tianjin-safety2.png" width="400" height="299" hspace="150" align="baseline" /><br clear="all" />
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              <span class="STYLE72"><span class="STYLE71"><strong>preventive&nbsp;measures&nbsp;</strong><br />
 +
              <strong>——How to avoid the occurrence of laboratory  accidents?</strong></span></span><span class="STYLE71"><strong></strong></span><strong><br />
 +
              </strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Laboratory safety issues can not be ignored.  Tianjin University started the “Lab-Safety Month of Tianjin  University”under the advocation of our IGEM team since 2013. A number of activities  were held to enhance students’ safety awareness. Here, we launched the  initiative that everyone pay more attention to laboratory safety, starting from  things around us and from ourselves.<br />
 +
              <img src="https://static.igem.org/mediawiki/2014/f/f2/Tianjin-safety3.png" width="400" height="299" hspace="150" />              <br clear="all" />
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              <span class="STYLE72"><strong>Safety Tips:</strong></span><br />
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1. Always wear  appropriate personal protective equipment. Feet and legs should be covered;  sandals and open-toed shoes should not be worn in laboratories. Wear  appropriate gloves while handling infectious or toxic materials and animals. Do  not wear lab coats, gloves or other personal protective equipment outside the  laboratory. Change gloves when contaminated, and dispose of used gloves with  other contaminated laboratory waste.<br />
 +
                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2. Do not eat, drink,  smoke, handle contact lenses, apply cosmetics, or store food for human  consumption in the laboratory.<br />
 +
                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3. Wash your hands  after working with potentially hazardous materials and before leaving the  laboratory.<br />
 +
                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;4. Follow the  institutional policies regarding safe handling of sharps (i.e., needles,  scalpels, pipettes, and broken glassware). Be careful with needles and  syringes. Use only when alternative methods are not feasible. Syringes,  needles, Pasteur pipettes, etc, should be placed in rigid, leak-proof  containers (Sharps Safe) and discarded following the waste rules.<br />
 +
                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;5. Take care to  minimize the creation of aerosols and/or splashes.<br />
 +
                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;6.Decontaminate  all work surfaces before and after your experiments, and immediately after any  spill or splash of potentially infectious material with an appropriate  disinfectant. Clean laboratory equipment routinely, even if it is not  contaminated.<br />
 +
                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;7. Decontaminate  all potentially infectious materials before disposal.<br />
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;8. Report any  incidents that may result in exposure to infectious materials to appropriate  personnel (e.g., laboratory supervisor, safety officer).<br />
 +
                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;9. Know where the  nearest eyewash, safety shower, and fire extinguisher are located. Know how to  use them.<br />
 +
                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;10. Insist upon  good housekeeping in your laboratory.<br />
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                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;11. Check for  insects and rodents. Keep all areas clean.<br />
 +
                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;12. Secure all gas  cylinders.<br />
 +
                &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;13. Several  species of toxic substances of biological laboratory we could often see in  laboratory: &nbsp;Tris,glycine,  X-gal, β-galactosidase.<br />
 +
                Not only we should pay more attention to  laboratory safety, but also should our governments enhance management.Welcome to visit <a href="https://2012.igem.org/Team:Tianjin/Safety">https://2012.igem.org/Team:Tianjin/Safety</a> for more details.</p>              </td>
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        <td height="56" bgcolor="#C67B14"><span class="STYLE29"><a name="part2" id="part2"></a><span class="STYLE62"><strong>②The safety of synthetic biology</strong></span></span></td>
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        <td height="794" bgcolor="#993300"><p><span class="STYLE33">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;International Risk Governance Council claims  that the risks caused by synthetic biology include:<br />
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          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(1) environmental risks,  such as bio-security;<br />
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          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(2) social risks; <br />
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          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(3) economic risks, such as  intellectual property rights; <br />
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          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;(4) the theoretical risks, such as natural or  unnatural products. Although synthetic biology allows we human beings to  respond to many aspects of challenges such as the global energy and  environment, its development and application process involves different levels  of security risk, which brings considerable social concern and worries.<br />
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              <span class="STYLE72"><strong>Our survey results</strong></span> <br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;To investigate the condition of synthetic  biology, we made a survey on the internet. The results show that faced with  safety issues of synthetic biology, 40.37% people indicate that they would follow scientific principles to  support the development of synthetic biology. 55.28% people support  precautionary principle, emphasizing the necessity to improve the supervision  mechanism. 4.35% showed an attitude that they do not care the safety problem,  they will use as long as they can be applied to life. As for whether should  people take part in the supervision and management of biological technology,  78.88% people answered “Yes”, 21.12% people disagree, they hold the opinion  that people are so lack of relevant biological knowledge that they cannot have  a comprehensive understanding of science.<br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;So we can see, a large number of people have the  desire to monitor the safety of synthetic biology. But it’s obvious that in the  management of synthetic biology, ordinary&nbsp;people make up too small parts  now. How to solve this contradiction? Our IGEM team had a heated discussion on  this issue. First of all, our government  and educational institution should promote the education and publicity of synthetic biology, to make more  people know what is synthetic biology and what does it do. Second, when a  synthetic biology product accesses to the&nbsp;market, the <a href="C://Users/lihuize/AppData/Local/Yodao/DeskDict/frame/20140810194504/javascript:void(0);"></a> producer must show basic principles and security of the product to the public,  &nbsp;which should be objective, full and accurate to make the information as  open and transparent as possible. Last but not least, we should establish a  comprehensive regulatory framework in our society, give people the rights to  &nbsp;supervise&nbsp;and&nbsp;manage the safety of synthetic biology. <br />
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              <span class="STYLE72"><strong>References</strong></span><br />
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              &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[1]LIU Xiao, TANG  Hongling, Synthetic biology and its safety supervision, Chemistry of Life? 2012,32(6)<br />
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        </span><span class="STYLE33">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[2] Li Zhihong, Research on  statistical analyses and countermeasures of 100 laboratory accidents,  Experimental Technology and Management 2014,31(4)            </span></p>
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        <td height="685" bgcolor="#C67B14"><span class="STYLE29"><a name="part3" id="part3"></a><strong class="STYLE62">③</strong><strong class="STYLE62">The safety of our project</strong><br />
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                      <td><p class="STYLE33">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Curli fiber  expressed by the bacterium Escherichia coli is a bacterial form of amyloid.  Amyloid fibers are associated with a variety of disease states, including prion  diseases and systemic amyloidosis. Curli fiber has a strong adhesive  power,?? can combine with the surface  material of many organisms, including fiber mucin, laminin, plasminogen and so  on. Vitro and in vivo tests showed that those bacteria? that be able to produce curli produce more  virulence than bacteria that don’t produce curli, including adhesion, planting  and invasion capacity of the host cell. E. Coli can produce curli fiber in its  mammalian host, which may bring related <a href="C://Users/lihuize/AppData/Local/Yodao/DeskDict/frame/20140810194504/javascript:void(0);"></a> inflammation like sepsis to its hosts. Luckily, the synthetic curli fiber in our project is  used outside of our body, so it will not bring such safety problem. Besides, in  the intact bacterium, the expression of curli requires the concerted action of  several gene products. Understanding how curli are assembled may help in our  understanding of pathological amyloids in human disease.<br />
 +
  &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Our project could be used to make  &nbsp;bioelectric&nbsp;detection, which could be applied in? the detection of some harmful substances to  the environment or our body. We hope it could make great contribution to some  hazardous substances’ safety problems in the near future.<br />
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  <span class="STYLE72"><strong>References</strong></span><br />
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                        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[1]Synthetic  Biologists Design ‘Living Materials’ That Build Themselves, Science28 MARCH 2014 1421.<br />
 +
                        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[2]XING Yan-ping,  WANG Yu, YANG Feng, LEILian-cheng, Pathogenicity of Escherichia coli Curli  Fibers, Journal of Hebei Normal University of Science &amp; Technology Vo.l 25  No. 1March, 2011.<br />
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                        <span class="STYLE33">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;[3]ZHU Jun, WANG Jian－Ye, ZHU Guo－Qiang, Biogenesis and  Pathogenicity of Escherichia coli Curli Fibers, LETTERS IN BIOTECHNOLOGY ,  Vol.20 No.1 Jan.2009</span></p>
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        <td width="19%" bgcolor="#3A2108"><img src="../images/tianjin-school.png" width="175" height="163" /></td>
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        <td width="81%" bgcolor="#3A2108"><p><span class="STYLE61">Our sponser:<br />
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        Tianjin University<br />
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          Eddress:
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          Tianjin University, Weijin street No.92, Tianjin, China<br />
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          Email:@edu.tju.cn</span></p>
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<p> Visit the <a href="https://2014.igem.org/Safety" >Safety Hub</a> to see this year's safety requirements. The Safety Hub is the central page for everything related to safety in iGEM. You can also go there to learn about general biosafety topics, and how to think about the future implications of your project.</p>
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<h3> Your Lab </h3>
 
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<p> Use this section to tell us about your laboratory. Where is it located? What sort of equipment do you use every day? Have you decorated it for the summer? How do you look wearing a lab coat? Take pictures! Show off your space! </p>
 
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Image:Example2_Lab_1.png|The building our lab is in!
 
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Image:Example2_Lab_2.png|The inside of our lab!
 
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Image:Example2_Lab_3.png|Team Member 3 doing an experiment
 
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Image:Example2_Lab_4.png|Working in biosafety cabinets
 
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Image:Example2_Lab_5.png|Team all gloved up and ready for work!
 
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Image:Example2_Lab_6.png|Equipment that we use to do SCIENCE!
 
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Image:Example2_Lab_7.png|We decorated this part of our lab
 
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Image:Example2_Lab_8.png|Whatever else you want
 
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<li> <b>Now :</b>  Read the <a href="https://2014.igem.org/Safety">Safety Hub </a> and learn about safety in iGEM. Ask questions by emailing safety at <i> igem DOT org </i>. </li>
 
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<li><b>Now - Jamboree:</b> Complete <b>Check-Ins</b> and receive approval before acquiring and using certain materials in your lab</li>
 
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<li><b>Now - Wiki Freeze:</b> Edit this Safety page to tell us about what you're doing</li>
 
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<li><b>June 9: </b>Submit the About Our Lab form.</li>
 
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<li><b>Let us know by June 25 </b>if you will need an extension on the Preliminary Version, or your Preliminary Version will be significantly incomplete.</li>
 
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<li><b>June 30: </b>Submit the Preliminary Version of the <b>Safety Form</b>.</li>
 
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<li>Participate in Virtual Open Office Hours to ask questions and discuss safety topics (exact date to be determined).</li>
 
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<li><b>September 1:</b> Submit the Final Version of the Safety Form.</li>
 
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<li><b>October: </b> Wiki freeze (exact date to be determined)</li>
 
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<li><b>October 30 - November 3: </b>GIANT JAMBOREE!</li>
 
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Revision as of 13:49, 14 October 2014


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<body>

<a href="#part1">The safety of lab</a>
<a href="#part2">The safety of synthetic biology</a>
<a href="#part3">The safety of our project</a>
<a href="#top">Go to the top</a>

</div>

<a href="#https://2014.igem.org/Main_Page"><img src="../images/Team：Tianjin image IGEM_official_logo.png" alt="image igem" width="202" height="114" border="0" align="top" /></a>

 

 

 

<a name="top" id="top"></a>Transf ibre

           Welcome to Team Tianjin!

<img src="Tianjin-head3.gif" width="456" height="226" align="right" />
<a href="#https://2014.igem.org/Team:Tianjin">Home|</a>
<a href="#">overview</a>
<a href="#" class="STYLE48">background</a>
<a href="#">design</a>
<a href="#" class="STYLE48">results</a>
<a href="#" class="STYLE48">future works </a>
<a href="#" class="STYLE48">modeling</a>
<a href="#https://2014.igem.org/Team:Tianjin/Project" class="STYLE48">Project|</a>
<a href="#" class="STYLE47">biobrick</a>
<a href="#" class="STYLE47">achievements</a>
<a href="#https://2014.igem.org/Team:Tianjin/Judging" class="STYLE47">Judging|</a>
<a href="#" class="STYLE47">methods</a>
<a href="#" class="STYLE47">labnotes</a>
<a href="#https://2014.igem.org/Team:Tianjin/Notebook" class="STYLE47">Notebook|</a>
<a href="#https://2014.igem.org/Team:Tianjin/Team">Team|</a>
<a href="#">gallery</a>
<a href="#" class="STYLE47">members</a>
<a href="#">attribution</a>
<a href="#" class="STYLE47">acknowledgment</a>
<a href="#https://2014.igem.org/Team:Tianjin/Safety">Safety|</a>
<a href="#" class="STYLE47">safety</a>
<a href="#" class="STYLE47">ethics</a>
<a href="#https://2014.igem.org/Team:Tianjin/HumanPractice">Human Practice|</a>
<a href="#">Weekly Newsletter</a>
<a href="#" class="STYLE56">A Nationwide Questionnaire</a>
<a href="#">Interaction Activities</a>


<a name="part1" id="part1"></a>①The safety of lab
Alarm bells
                      According to the Ministry of Education “2008-2009  General Undergraduate Colleges Laboratory Information Statistics”, there are  26479 laboratories in 1129 total regular undergraduate colleges in China,  laboratory area 2 6.015 million m2. With so many  laboratories, safety accidents have occured from time to time. In 2009, a  university teacher of the department of chemistry mistakenly accessed CO gas  into 307 lab, which should have been accessed to 211 lab. A woman doctor died  because of this fault; In 2010, students and teachers of a university in  northeast of China used goats without being checked in their experiments, which  lead 27 students and 1 teacher were diagnosed as infected with brucellosis; In  2013,  a postgraduate student named Huang Yang from Fudan University died  of poisoning in Shanghai Zhongshan Hospital. According to the report of the  police's investigation, a toxic chemical compound was found in the drinking  water dispenser in Huang's dormitory room, and this toxic chemical compoud is  taken out of a laboratory.

             <img src="Tianjin-safety.png" width="400" height="264" hspace="150" align="middle" />

Ponder why
——Why do such tragedies repeat again and again?
        These terrible incidents are not mere coincidences and it reminds us college students of the importance of lab-safety. Thus we studied a large number of reports and literature to analyze the causes of? laboratory accidents. First of all, leaders and managers pay insufficient attention is the main reason. Second, management system and mechanism are not sound. Lacking of safety consciousness also cannot be neglected in causing safety problems. Last but not least, non-standard hardware construction is also a key reason.
reflect on our own
——What is happening to our lab?
        Laboratory safety awareness is the "Achilles heel" to our lab. Too much facts tell us that most accidents are caused by negligence of researchers. Do experiments without wearing gloves or lab coats, pile up clutter at random in the laboratory, or eat pineapples in the laboratory during experimental clearances. Now ask yourself, have these phenomenon happened to you or around you on earth before?
<img src="Tianjin-safety2.png" width="400" height="299" hspace="150" align="baseline" />
preventive measures 
——How to avoid the occurrence of laboratory accidents?
        Laboratory safety issues can not be ignored. Tianjin University started the “Lab-Safety Month of Tianjin University”under the advocation of our IGEM team since 2013. A number of activities were held to enhance students’ safety awareness. Here, we launched the initiative that everyone pay more attention to laboratory safety, starting from things around us and from ourselves.
<img src="Tianjin-safety3.png" width="400" height="299" hspace="150" />
Safety Tips:
        1. Always wear appropriate personal protective equipment. Feet and legs should be covered; sandals and open-toed shoes should not be worn in laboratories. Wear appropriate gloves while handling infectious or toxic materials and animals. Do not wear lab coats, gloves or other personal protective equipment outside the laboratory. Change gloves when contaminated, and dispose of used gloves with other contaminated laboratory waste.
        2. Do not eat, drink, smoke, handle contact lenses, apply cosmetics, or store food for human consumption in the laboratory.
        3. Wash your hands after working with potentially hazardous materials and before leaving the laboratory.
        4. Follow the institutional policies regarding safe handling of sharps (i.e., needles, scalpels, pipettes, and broken glassware). Be careful with needles and syringes. Use only when alternative methods are not feasible. Syringes, needles, Pasteur pipettes, etc, should be placed in rigid, leak-proof containers (Sharps Safe) and discarded following the waste rules.
        5. Take care to minimize the creation of aerosols and/or splashes.
        6.Decontaminate all work surfaces before and after your experiments, and immediately after any spill or splash of potentially infectious material with an appropriate disinfectant. Clean laboratory equipment routinely, even if it is not contaminated.
        7. Decontaminate all potentially infectious materials before disposal.
        8. Report any incidents that may result in exposure to infectious materials to appropriate personnel (e.g., laboratory supervisor, safety officer).
        9. Know where the nearest eyewash, safety shower, and fire extinguisher are located. Know how to use them.
        10. Insist upon good housekeeping in your laboratory.
        11. Check for insects and rodents. Keep all areas clean.
        12. Secure all gas cylinders.
        13. Several species of toxic substances of biological laboratory we could often see in laboratory:  Tris,glycine, X-gal, β-galactosidase.
Not only we should pay more attention to laboratory safety, but also should our governments enhance management.Welcome to visit <a href="https://2012.igem.org/Team:Tianjin/Safety">https://2012.igem.org/Team:Tianjin/Safety</a> for more details.
<a name="part2" id="part2"></a>②The safety of synthetic biology

        International Risk Governance Council claims that the risks caused by synthetic biology include:

                 (1) environmental risks,  such as bio-security;
 
                 (2) social risks; 

                 (3) economic risks, such as  intellectual property rights; 

                 (4) the theoretical risks, such as natural or  unnatural products. Although synthetic biology allows we human beings to  respond to many aspects of challenges such as the global energy and  environment, its development and application process involves different levels  of security risk, which brings considerable social concern and worries.

             Our survey results 

                     To investigate the condition of synthetic  biology, we made a survey on the internet. The results show that faced with  safety issues of synthetic biology, 40.37% people indicate that they would follow scientific principles to  support the development of synthetic biology. 55.28% people support  precautionary principle, emphasizing the necessity to improve the supervision  mechanism. 4.35% showed an attitude that they do not care the safety problem,  they will use as long as they can be applied to life. As for whether should  people take part in the supervision and management of biological technology,  78.88% people answered “Yes”, 21.12% people disagree, they hold the opinion  that people are so lack of relevant biological knowledge that they cannot have  a comprehensive understanding of science.

                     So we can see, a large number of people have the  desire to monitor the safety of synthetic biology. But it’s obvious that in the  management of synthetic biology, ordinary people make up too small parts  now. How to solve this contradiction? Our IGEM team had a heated discussion on  this issue. First of all, our government  and educational institution should promote the education and publicity of synthetic biology, to make more  people know what is synthetic biology and what does it do. Second, when a  synthetic biology product accesses to the market, the <a href="C://Users/lihuize/AppData/Local/Yodao/DeskDict/frame/20140810194504/javascript:void(0);"></a> producer must show basic principles and security of the product to the public,   which should be objective, full and accurate to make the information as  open and transparent as possible. Last but not least, we should establish a  comprehensive regulatory framework in our society, give people the rights to   supervise and manage the safety of synthetic biology. 

             References

                     [1]LIU Xiao, TANG  Hongling, Synthetic biology and its safety supervision, Chemistry of Life? 2012,32(6)
        [2] Li Zhihong, Research on statistical analyses and countermeasures of 100 laboratory accidents, Experimental Technology and Management 2014,31(4)

<a name="part3" id="part3"></a>③The safety of our project

        Curli fiber expressed by the bacterium Escherichia coli is a bacterial form of amyloid. Amyloid fibers are associated with a variety of disease states, including prion diseases and systemic amyloidosis. Curli fiber has a strong adhesive power,?? can combine with the surface material of many organisms, including fiber mucin, laminin, plasminogen and so on. Vitro and in vivo tests showed that those bacteria? that be able to produce curli produce more virulence than bacteria that don’t produce curli, including adhesion, planting and invasion capacity of the host cell. E. Coli can produce curli fiber in its mammalian host, which may bring related <a href="C://Users/lihuize/AppData/Local/Yodao/DeskDict/frame/20140810194504/javascript:void(0);"></a> inflammation like sepsis to its hosts. Luckily, the synthetic curli fiber in our project is used outside of our body, so it will not bring such safety problem. Besides, in the intact bacterium, the expression of curli requires the concerted action of several gene products. Understanding how curli are assembled may help in our understanding of pathological amyloids in human disease.

         Our project could be used to make   bioelectric detection, which could be applied in? the detection of some harmful substances to  the environment or our body. We hope it could make great contribution to some  hazardous substances’ safety problems in the near future.

 References

                               [1]Synthetic  Biologists Design ‘Living Materials’ That Build Themselves, Science28 MARCH 2014 1421.

                               [2]XING Yan-ping,  WANG Yu, YANG Feng, LEILian-cheng, Pathogenicity of Escherichia coli Curli  Fibers, Journal of Hebei Normal University of Science & Technology Vo.l 25  No. 1March, 2011.
        [3]ZHU Jun, WANG Jian－Ye, ZHU Guo－Qiang, Biogenesis and Pathogenicity of Escherichia coli Curli Fibers, LETTERS IN BIOTECHNOLOGY , Vol.20 No.1 Jan.2009


<img src="../images/tianjin-school.png" width="175" height="163" />

Our sponser:

       Tianjin University

         Eddress:
         Tianjin University, Weijin street No.92, Tianjin, China
Email:@edu.tju.cn

 



 

 

 

 

 

 


 

 

 

 

 

 

 



 

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