Team:UGA-Georgia/Notebook
From 2014.igem.org
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<li> Made frozen stocks of RBS colonies, </li> | <li> Made frozen stocks of RBS colonies, </li> | ||
<li> Sub-cultured parent strains. </li> | <li> Sub-cultured parent strains. </li> | ||
+ | <li> Researched primary literature on mCherry maturation. </li> | ||
+ | <li> Began developing a protocol for oxygen maturation of mCherry. </li> | ||
+ | <br> | ||
+ | <h5> Week 3 </h5> | ||
+ | <li> Made frozen stocks of RBS colonies and sub-cultured parent strains. </li> | ||
+ | <li> Re-subcultured and revive pAW50-mcherry. </li> | ||
+ | <li> Subcultured the parent strains of WT and native RBS (12) into triplicates. The triplicates were wrapped in foil to prevent any photobleaching. </li> | ||
+ | <li> The pAW50-mcherry came up and then all samples were taken to a plate reader to perform fluorescence tests. </li> | ||
+ | <br> | ||
+ | <h5> Week 5 </h5> | ||
+ | |||
</td> | </td> |
Revision as of 13:22, 14 October 2014
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Notebook | |||||
FebruaryWeek 1Week 3Week 4MarchWeek 1Week 3AprilWeek 1Week 2Week 3MayWeek 4JuneWeek 1Week 2Week 3Week 4JulyWeek 1Week 2Week 3Week 5 |