Team:NCTU Formosa/Notebook/test7

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Revision as of 12:30, 14 October 2014

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10/3

Cultivation of

[Pcons+RBS+NdeI]Cr

[Pcons+RBS+NcoI]Cr

[KpnI-1]Cr

[NdeI-1]Cr

[XhoI-1]Cr

[SacI-1]Cr

[AseI-1]Cr

[ Pcons+RBS+HindIII-1]Cr

[ Pcons+RBS+KpnI-4]Cr

[ Pcons+RBS+AseI-5]Cr

[ Pcons+RBS+XhoI-1]Cr

[ Pcons+RBS+SacI-1]Cr

[ Pcons+RBS+KpnI-1+J61048]Cr

[SalI-4]Cr

[Pcons+RBS+SalI-3+J61048]Cr

[HindIII-4]Cr

[Pcons+RBS+AseI+J61048]Cr

[NheI]

[NcoI]

Electrophoresis of

[NdeI](EP)

[NcoI](EP)

[SacI](EP)

Transformation of

[Pcons+RBS+NdeI]Cr

[Pcons+RBS+SacI]Cr

DNA Sequencing of

[Pcons+RBS+NdeI+J61048-1]

[ Pcons+RBS+HindIII+J61048-1]

[ Pcons+RBS+SalI+J61048-4]

[Pcons+RBS+SacI+J61048-1]

[Pcons+RBS+SacI+J61048-1](fail)

10/4

Digestion of

[Pcons+RBS+XhoI+J61048-2, -4, -5]

[Pcons+RBS+SacI+J61048-10, -11]

[Pcons+RBS+NheI+J61048-20]

[Pcons+RBS+NheI]Cr

[Pcons+RBS+NcoI]Cr

Cultivation of

[Pcons+RBS+NheI-1, -2, -4, -6, -10, -12]

[Pcons+RBS+SalI-1, -3, -6, -7, -11, -12]

Cultivation of

[Pcons+RBS+NcoI+J61048-1~8]

PCR of

[Pcons+RBS+NheI-1, -2, -4, -6, -10, -12]

[Pcons+RBS+SacI-1, -3, -6, -7, -11, -12]

[Pcons+RBS+NcoI+J61048-1~8]

Ligation of

[Pcons+RBS+NheI]Kr

[ Pcons+RBS+NcoI]Kr

Electrophoresis of

[XhoI-2, -4, -5](dig)

[SacI-10, -11](dig)

[NheI-20](dig)

[Pcons+RBS+NdeI](dig)

[Pcons+RBS+NcoI](dig)

[PCR NcoI-1~8]

Mini-prep of

[Pcons+RBS+NheI-6]

[Pcons+RBS+SalI-1, -7, -11]

Digestion of

[Pcons+RBS+NheI-6](EP)

[Pcons+RBS+SalI-1, -7, -11](EP)

[Pcons+RBS+NcoI]

[Pcons+RBS+Ndel]

PCR of and cultivation of

[Pcons+RBS+NcoI+J61048]

Mini-prep of

[Pcons+RBS+NcoI+J61048-7]Cr

Electrophoresis of

[Pcons+RBS+KphI+J61048-1]Cr

[ Pcons+RBS+SalI+J61048-3]Cr

[ Pcons+RBS+AseI+J61048 ]Cr

[Pcons+RBS+NdeI+J61048-1]

[ Pcons+RBS+HindIII+J61048-1]

[ Pcons+RBS+SacI+J61048 -1]

[Pcons+RBS+NcoI+J61048](dig)

[SacI-11]

DNA Sequencing of

[Pcons+RBS+NdeI]-2 Cr

[Pcons+RBS+NcoI]-2 Cr

[Pcons+RBS+NheI]-6 Cr

[Pcons+RBS+SalI-1]Cr

[Pcons+RBS+SalI-11]Cr

[Pcons+RBS+HindIII-1]Cr

[NcoI-12]

[ Pcons+RBS+KpnI-1+J61048]Cr

[ Pcons+RBS+AseI+J61048]Cr

[ Pcons+RBS+SalI-3+J61048]Cr

[ Pcons+RBS+HindIII-1+J61048]Cr

[ Pcons+RBS+XhoI-2+J61048]Cr

[ Pcons+RBS+XhoI-5+J61048]Cr

[ Pcons+RBS+NcoI-5+J61048]Cr

[ Pcons+RBS+SacI-11-1+J61048]Cr

[ Pcons+RBS+NdeI-1+J61048]Cr

[ Pcons+RBS+NheI-20+J61048]Cr

[ Pcons+RBS+AseI-7+J61048]Cr

[ Pcons+RBS+HindIII-7+J61048]Ar

[ Pcons+RBS+SacI-16+J61048]Ar

[ Pcons+RBS+SalI-17+J61048]Ar

[ Pcons+RBS+NdeI-2+J61048]Cr

[ Pcons+RBS+NcoI-2+J61048]Cr

[ Pcons+RBS+XhoI+J61048]Ar

[ Pcons+RBS+KpnI-7+J61048]Ar

[ Pcons+RBS+NheI-11+J61048]Ar

10/7

PCR of

[ Pcons+RBS+NheI+J61048]

[ Pcons+RBS+SalI in PSB3C5]

[Pcons+RBS+SacI in PSB3C5]

Cultivation of

[Pcons+RBS+NheI+J61048-1, -3, -4, -6, -8, -10, -15, -16]

Transformation of

[Pcons+RBS+XhoI-2 +J61048]

[Pcons+RBS+XhoI-5 +J61048-1]

[Pcons+RBS+SacI-11+J61048]

PCR of

[Pcons+RBS+ NdeI in PSB3C5]

[Pcons+RBS+KpnI in PSB3C5]

[Pcons+RBS+AseI in PSB3C5]

[Pcons+RBS+XhoI in PSB3C5]

[Pcons+RBS+NcoI in PSB3C5]

[Pcons+RBS+NheI in PSB3C5]

nine kinds of PBAN

DNA Sequencing of

[Pcons+RBS+NcoI-5+J61048]

10/8

Sequencing of

[PBAN NcoI]

[Pcons+RBS+SalI -1]

[Pcons+RBS+SalI-11]

[Pcons+RBS+NheI-6]

[Pcons+RBS+xhol-2+RBS+BFP+TER]

[Pcons+RBS+SacI-11+RBS+BFP+TER]

[Pcons+RBS+ Nhel-20+RBS+BFP+TER]

Ligation of

Pcons+RBS+NcoI on psb3c5

10/9

ALL Nhel-9(10/7)x2

ALL SacI-11(10/7)x1

ALL xhol-2(10/7)x1

Electrophoresis of

Gel 1

Well1 Marker

Well2 Pcons+RBS+NheI-20+RBS+BFP+TER

Well3 Pcons+RBS+Hind-1+RBS+BFP+TER

Well4 Pcons+RBS+KpnI-1+RBS+BFP+TER

Well5 Pcons+RBS+Ndel-1+RBS+BFP+TER

2 Well6 Pcons+RBS+NcoI-5+RBS+BFP+TER

Well7 Pcons+RBS+AseI ALL

Well8 PBAN NcoI-12

Well9 Pcons+RBS+NheI-6

Well10 Pcons+RBS+Hind-1

Well11 Pcons+RBS+NcoI-20

Well12 Pcons+RBS+NcoI-2-2

Well13 Pcons+RBS+Ndel-2-1

Well14 Pcons+RBS+Nedl-2-2

Well15 Pcons+RBS+SacI-1

Well16 Pcons+RBS+SacI-11

Well17 Marker

Gel 2

Well1 marker

Well2 Pcons+RBS+NheI-11

Well3 Pcons+RBS+Hind3

Well4 Pcons+RBS+KpnI-7

Well5 Pcons+RBS+SacI-16

Well6 Pcons+RBS+SalI-12

Well7 Pcons+RBS+Xhol

Transformation of

Pcons+RBS+SacI-3 on psb3c5

Pcons+RBS+KpnI-1 on psb3c5

Pcons+RBS+NcoI-1 on psb3c5

Mini-prep of and sequencing of

ALL NheI-2 x1

ALL SacI-11 x1

ALL Xhol-2 X1

10/10

Transformation of

Pcons+RBS+Ndel on psb3c5

Pcons+RBS+NcoI on psb3c5

Electrophoresis of

Well1 marker

Well2 Pcons+RBS+SacI-3 on psb3c5

Well3 Pcons+RBS+KpnI-1 on psb3c5

Well4 Pcons+RBS+NcoI-1 on psb3c5

10/12

PCR of and Electrophoresis of

Well1 Pcons+RBS+NcoI+psb3c5

Well2 Pcons+RBS+Ndel+psb3c5

4

5

@@ Line 74: / Line 74: @@
 <div class="single note">
 <p>
-/3</p><p>
+<font color="#FF0000"><span style="font-size:1cm;">10/3</span></font><br></p><p>
-Cultivation of</p><p>
+<font color="yellow">Cultivation of</font></p><p>
 [Pcons+RBS+NdeI]Cr</p><p>
 [Pcons+RBS+NcoI]Cr</p><p>
@@ Line 95: / Line 95: @@
   [NheI]</p><p>
   [NcoI]</p><p>
-Electrophoresis of</p><p>
+<font color="yellow">Electrophoresis of</font></p><p>
   [NdeI](EP)</p><p>
   [NcoI](EP)</p><p>
   [SacI](EP)</p><p>
-Transformation of</p><p>
+<font color="yellow">Transformation of</font></p><p>
   [Pcons+RBS+NdeI]Cr</p><p>
   [Pcons+RBS+SacI]Cr</p><p>
-Sequencing of</p><p>
+<font color="yellow">DNA Sequencing of</font></p><p>
   [Pcons+RBS+NdeI+J61048-1]</p><p>
   [ Pcons+RBS+HindIII+J61048-1]</p><p>
@@ Line 108: / Line 108: @@
   [Pcons+RBS+SacI+J61048-1]</p><p>
 [Pcons+RBS+SacI+J61048-1](fail)</p><p>
-/4</p><p>
+<font color="#FF0000"><span style="font-size:1cm;">10/4</span></font><br></p><p>
-Digestion of</p><p>
+<font color="yellow">Digestion of</font></p><p>
   [Pcons+RBS+XhoI+J61048-2, -4, -5] </p><p>
   [Pcons+RBS+SacI+J61048-10, -11]</p><p>
@@ Line 115: / Line 115: @@
   [Pcons+RBS+NheI]Cr</p><p>
   [Pcons+RBS+NcoI]Cr</p><p>
-Cultivation of </p><p>
+<font color="yellow">Cultivation of </font></p><p>
 [Pcons+RBS+NheI-1, -2, -4, -6, -10, -12]</p><p>
   [Pcons+RBS+SalI-1, -3, -6, -7, -11, -12] </p><p>
-Cultivation of [Pcons+RBS+NcoI+J61048-1~8]</p><p>
+<font color="yellow">Cultivation of </font></p><p>
-PCR of</p><p>
+[Pcons+RBS+NcoI+J61048-1~8]</p><p>
+<font color="yellow">PCR of</font></p><p>
   [Pcons+RBS+NheI-1, -2, -4, -6, -10, -12]</p><p>
   [Pcons+RBS+SacI-1, -3, -6, -7, -11, -12]</p><p>
   [Pcons+RBS+NcoI+J61048-1~8]</p><p>
-Ligation of </p><p>
+<font color="yellow">Ligation of </font></p><p>
 [Pcons+RBS+NheI]Kr</p><p>
   [ Pcons+RBS+NcoI]Kr</p><p>
-Electrophoresis of </p><p>
+<font color="yellow">Electrophoresis of </font></p><p>
 [XhoI-2, -4, -5](dig)</p><p>
   [SacI-10, -11](dig)</p><p>
@@ Line 133: / Line 134: @@
   [Pcons+RBS+NcoI](dig)</p><p>
   [PCR NcoI-1~8]</p><p>
-Mini-prep of</p><p>
+<font color="yellow">Mini-prep of</font></p><p>
   [Pcons+RBS+NheI-6]</p><p>
   [Pcons+RBS+SalI-1, -7, -11]</p><p>
-Digestion of </p><p>
+<font color="yellow">Digestion of </font></p><p>
 [Pcons+RBS+NheI-6](EP)</p><p>
   [Pcons+RBS+SalI-1, -7, -11](EP)</p><p>
   [Pcons+RBS+NcoI]</p><p>
   [Pcons+RBS+Ndel]</p><p>
-PCR and cultivation of </p><p>
+<font color="yellow">PCR of and cultivation of </font></p><p>
 [Pcons+RBS+NcoI+J61048]</p><p>
-Mini-prep of</p><p>
+<font color="yellow">Mini-prep of</font></p><p>
   [Pcons+RBS+NcoI+J61048-7]Cr</p><p>
-Electrophoresis of </p><p>
+<font color="yellow">Electrophoresis of </font></p><p>
 [Pcons+RBS+KphI+J61048-1]Cr</p><p>
   [ Pcons+RBS+SalI+J61048-3]Cr</p><p>
@@ Line 155: / Line 156: @@
   [Pcons+RBS+NcoI+J61048](dig)</p><p>
   [SacI-11]</p><p>
-Sequencing of </p><p>
+<font color="yellow">DNA Sequencing of </font></p><p>
 [Pcons+RBS+NdeI]-2 Cr</p><p>
 [Pcons+RBS+NcoI]-2 Cr</p><p>
@@ Line 185: / Line 186: @@
 <div class="single note">
 <p>
-/7</p><p>
+<font color="#FF0000"><span style="font-size:1cm;">10/7</span></font><br></p><p>
-PCR of</p><p>
+<font color="yellow">PCR of</font></p><p>
   [ Pcons+RBS+NheI+J61048]</p><p>
 [ Pcons+RBS+SalI in PSB3C5]</p><p>
   [Pcons+RBS+SacI in PSB3C5]</p><p>
-Cultivation of </p><p>
+<font color="yellow">Cultivation of</font> </p><p>
 [Pcons+RBS+NheI+J61048-1, -3, -4, -6, -8, -10, -15, -16]</p><p>
-Transformation of </p><p>
+<font color="yellow">Transformation of </font></p><p>
 [Pcons+RBS+XhoI-2 +J61048]</p><p>
   [Pcons+RBS+XhoI-5 +J61048-1]</p><p>
   [Pcons+RBS+SacI-11+J61048]</p><p>
-PCR of</p><p>
+<font color="yellow">PCR of</font></p><p>
    [Pcons+RBS+ NdeI  in PSB3C5]</p><p>
   [Pcons+RBS+KpnI in PSB3C5]</p><p>
@@ Line 204: / Line 205: @@
   [Pcons+RBS+NheI in PSB3C5]</p><p>
   nine kinds of PBAN</p><p>
-Sequencing of</p><p>
+<font color="yellow">DNA Sequencing of</font></p><p>
    [Pcons+RBS+NcoI-5+J61048]</p><p>
-/8 </p><p>
+<font color="#FF0000"><span style="font-size:1cm;">10/8</span></font><br> </p><p>
-Sequencing of</p><p>
+<font color="yellow">Sequencing of</font></p><p>
 [PBAN NcoI]</p><p>
 [Pcons+RBS+SalI -1]</p><p>
@@ Line 216: / Line 217: @@
 [Pcons+RBS+ Nhel-20+RBS+BFP+TER]</p>
 <p>
-Ligation of</p><p>
+<font color="yellow">Ligation of</font></p><p>
 Pcons+RBS+NcoI on psb3c5</p><p>
 Pcons+RBS+NcoI on psb3c5</p><p>
-/9</p><p>
+<font color="#FF0000"><span style="font-size:1cm;">10/9</span></font><br></p><p>
 ALL Nhel-9(10/7)x2</p><p>
 ALL SacI-11(10/7)x1</p><p>
 ALL xhol-2(10/7)x1</p><p>
-Electrophoresis of</p><p>
+<font color="yellow">Electrophoresis of</font></p><p>
-gel 1</p><p>
+Gel 1</p><p>
 Well1	Marker</p><p>
 Well2	Pcons+RBS+NheI-20+RBS+BFP+TER</p><p>
 Well3	Pcons+RBS+Hind-1+RBS+BFP+TER</p><p>
 Well4	Pcons+RBS+KpnI-1+RBS+BFP+TER </p><p>
-Well5	Pcons+RBS+Ndel-1+RBS+BFP+TER</p><p>
+Well5	Pcons+RBS+Ndel-1+RBS+BFP+TER</p><p>2
 Well6	Pcons+RBS+NcoI-5+RBS+BFP+TER</p><p>
 Well7	Pcons+RBS+AseI ALL</p><p>
@@ Line 242: / Line 243: @@
 Well16	Pcons+RBS+SacI-11</p><p>
 Well17	Marker</p><p>
-gel 2</p><p>
+Gel 2</p><p>
 Well1	marker</p><p>
 Well2	Pcons+RBS+NheI-11</p><p>
@@ Line 250: / Line 251: @@
 Well6	Pcons+RBS+SalI-12</p><p>
 Well7	Pcons+RBS+Xhol</p><p>
-Transformation of</p><p>
+<font color="yellow">Transformation of</font></p><p>
 Pcons+RBS+SacI-3 on psb3c5</p><p>
 Pcons+RBS+KpnI-1 on psb3c5</p><p>
@@ Line 256: / Line 257: @@
 </p><p>
-Mini-prep and sequencing</p><p>
+<font color="yellow">Mini-prep of and sequencing of</font></p><p>
 ALL NheI-2 x1</p><p>
 ALL SacI-11 x1</p><p>
 ALL Xhol-2 X1</p><p>
-/10</p><p>
+<font color="#FF0000"><span style="font-size:1cm;">10/10</span></font><br></p><p>
-Transformation of </p><p>
+<font color="yellow">Transformation of </font></p><p>
 Pcons+RBS+Ndel on psb3c5</p><p>
 Pcons+RBS+NcoI on psb3c5</p><p>
-Electrophoresis of </p><p>
+<font color="yellow">Electrophoresis of </font></p><p>
 Well1	marker</p><p>
@@ Line 275: / Line 276: @@
 <div class="single note">
 <p>
-/10</p><p>
+<font color="#FF0000"><span style="font-size:1cm;">10/12</span></font><br></p><p>
-Transformation of</p><p>
+<font color="yellow">PCR of and Electrophoresis of</font></p><p>
-Pcons+RBS+Ndel on psb3c5</p><p>
-Pcons+RBS+NcoI on psb3c5</p><p>
-Electrophoresis of</p><p>
-Well1	marker</p><p>
-Well2	Pcons+RBS+SacI-3 on psb3c5</p><p>
-Well3	Pcons+RBS+KpnI-1 on psb3c5</p><p>
-Well4	Pcons+RBS+NcoI-1 on psb3c5</p><p>
-/12</p><p>
-<font color="yellow">PCR and Electrophoresis of</font></p><p>
 Well1	Pcons+RBS+NcoI+psb3c5</p><p>