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| Line 24: |
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| | start: new Date(2014,6,09), | | start: new Date(2014,6,09), |
| | description: "Result: Plasmid prep yielded 4x100uL tubes<br><br>- Rokiah"}, | | description: "Result: Plasmid prep yielded 4x100uL tubes<br><br>- Rokiah"}, |
| - | { title: "Plasmid prep of pUS44, Checking: Setup restriction digest and incubate",
| |
| - | start: new Date(2014,6,09),
| |
| - | description: "Details: restriction digest to check plasmids (EcoR1, Ssp1, null). Incubated at 37C overnight.<br><br>- Abi"},
| |
| - | { title: "Plasmid prep of pUS44, Checking: finish digests, gel electrophoresis of digests",
| |
| - | start: new Date(2014,6,10),
| |
| - | description: "Details: Gel electrophoresis (100V, 400mA, 30min)<br><br>Result: Gel broke. <br><br>- Abi"},
| |
| - | { title: "Plasmid prep of psB6A1, Checking: finish digests, gel electrophoresis of digests",
| |
| - | start: new Date(2014,6,10),
| |
| - | description: "Details: Gel electrophoresis (100V, 400mA, 30min)<br><br>Result: Gel broke. pSB6A1 yielded 5104.1ng/uL DNA & (A260/280) 1.62.<br><br>Conclusion: Possible protein contamination to samples<br><br>- Abi"},
| |
| - | { title: "Plasmid prep of pSB6A1, Re-doing digest",
| |
| - | start: new Date(2014,6,11),
| |
| - | description: "Details: Restriction digest to check plasmids (EcoR1, EcoR1+Pst1, EcoR1+Nde1). <br><br>- Jeanne"},
| |
| - | { title: "Plasmid prep of pUS44, Re-doing digest",
| |
| - | start: new Date(2014,6,15),
| |
| - | description: "Details: Restriction digest to check plasmids (EcoR1, EcoR1+Pst1, EcoR1+Nde1). <br><br>- Abi"},
| |
| - | { title: "Plasmid prep of pUS44, Re-doing digest",
| |
| - | start: new Date(2014,6,17),
| |
| - | description: "Details: Restriction digest. Did the protocol change?<br><br>Conclusion: Nanospec: 395.5ng/mL DNA yield<br><br>- Abi & Tom"},
| |
| - | { title: "Construction of pUS201, PCR of pSB6A1 using iGEM401/402 primers ",
| |
| - | start: new Date(2014,6,25),
| |
| - | description: "Details: PCR Master mix with 1401/1402 primers. Used iGEM pSB6A1. Ran on regular program PCR ~2HRS<br><br>Result: Yield 1018.6ng/uL and low 260/280<br><br>Conclusion: ?<br><br>- Callum"},
| |
| - | { title: "Construction of pUS201, Checking: finish pcr, gel electrophoresis of digests",
| |
| - | start: new Date(2014,6,25),
| |
| - | description: "Details: Gel electrophoresis (100V, 400mA, 1hr). Stained in gel red for 30min<br><br>Result: Expected fragment size of 2.6kB from pSB6A1<br><br>Conclusion: Primers amplified expected product from pSB6A1<br><br>- Callum"},
| |
| - | { title: "Construction of pUS204, Restriction digest of pSB1C3",
| |
| - | start: new Date(2014,6,30),
| |
| - | description: "Details: Restriction digest (EcoR1+Pst1)<br><br>Result: Yield 25ng/mL<br><br>Conclusion: Enough to use for gibson assembly<br><br>- Callum"},
| |
| - | { title: "Construction of pUS201, PCR of pSB1C3 using iGEM403/1404 primers ",
| |
| - | start: new Date(2014,6,31),
| |
| - | description: "Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Yield 329.4ng/uL, low 260/280<br><br>- Callum"},
| |
| - | { title: "Construction of pUS204, Transformation: pUS204 into top10 cells",
| |
| - | start: new Date(2014,6,31),
| |
| - | description: "Details: Transformation (competent top10, pUS204). Incubated on AmpR plates for 2hrs<br><br>Result: Some AmpR colonies present, no blue colonies detected<br><br>Conclusion: pUS024 did not successfully transform, \"far unlikely positive control worked\" <br><br>- ?"},
| |
| - | { title: "construction of pUS201, see notebook: second GA and transformation successful???",
| |
| - | start: new Date(2014,7,01),
| |
| - | description: ""},
| |
| - | { title: "construction of pUS201, re-doing pSB1C3 PCR with iGEM1403/1404",
| |
| - | start: new Date(2014,7,04),
| |
| - | description: "Details: PCR Master mix with 1403/1404 primers. Used iGEM psB1C3. Ran on regular program PCR ~2HRS<br><br>Result: Products stored in the fridge<br><br>- Jeanne & Callum"},
| |
| - | { title: "construction of pus201, Gel purification: gel electrophoresis of pSB1C3 PCR ",
| |
| - | start: new Date(2014,7,05),
| |
| - | description: "Details: Gel purification electrophoresis (60V, 400mA, 1hr). Stained in gel red??? for 30min<br><br>Result: missing gel??<br><br>- Jeanne & Callum"},
| |
| - | { title: "Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies",
| |
| - | start: new Date(2014,7,05),
| |
| - | description: "Details: Incubated overnight to finish later<br><br>Result: Yield 5.7ng/ul, 1.61 260/280<br><br>Conclusion: Yield too low to use<br><br>- Jeanne & Callum"},
| |
| - | { title: "Checking IntI1 from SamR\'s construct, Restriction digest: SamR\'s pSB1C3+ARApBAD",
| |
| - | start: new Date(2014,7,08),
| |
| - | description: "Details: Restriction digest (EcoR1+Pst1), incubated at 37 C for 1hr<br><br>Result: Yield: 4.46 ug/uL. Stored in -20 C fridge<br><br>Conclusion: Yield much higher than estimate provided by SamR (50ng/uL). Useable<br><br>- Rokiah"},
| |
| - | { title: "Checking pUS201 construct, Checking pUS201 with digests",
| |
| - | start: new Date(2014,7,12),
| |
| - | description: "Details: Restriction Digest (uncut, EcoR1, HindIII, HindIII+BamHI)<br><br>Result: gel????? Results???<br><br>- ?"},
| |
| - | { title: "Checking IntI1 from SamR\'s construct, Checking: SamR\'s digests on gel electrohoresis",
| |
| - | start: new Date(2014,7,12),
| |
| - | description: "Details: Gel electrophoresis (Spe1, uncut) at 120V for 1hr. Stained in gel red for ~30min.<br><br>Result: [12.08 SamR gel]. Cut lanes travelled further than uncut, as expected.<br><br>Conclusion: NOTE:: notes at the end cannot be read<br><br>- Rokiah"},
| |
| - | { title: "Making IntI1 for pUS203, Restriction Digest: extracting SamR\'s AraC-pBAD from pSB1C3",
| |
| - | start: new Date(2014,7,13),
| |
| - | description: "Details: pSB1C3 with AraC-pBAD was incubated with *specific enzyme* at 80 C for 20 min. The enzyme was heat-killed<br><br>- andy"},
| |
| - | { title: "Construction of pUS204, Restrction digestion of aeBlue gBlock",
| |
| - | start: new Date(2014,7,14),
| |
| - | description: "Details: Restriction Digestion (EcoR1+Pst1)<br><br>Result: Yield: 1.6ng/uL<br><br>Conclusion: Enough yield to continue with ligation<br><br>- abi and tom"},
| |
| - | { title: "Checking pUS201 construct, Plasmid mini-Prep: to confirm pUS201 in top10 colonies",
| |
| - | start: new Date(2014,7,21),
| |
| - | description: "Details: Plasmid prep<br><br>Result: Yield: 3680.1ng/uL. 2.03 A260/280<br><br>Conclusion: A260/280 high ratio, may have contamination<br><br>- Callum"},
| |
| - | { title: "Construction of pUS204, Ligation of pSB1C3 and aeBlue gBlock digestion products",
| |
| - | start: new Date(2014,7,28),
| |
| - | description: "Details: Ligation using T4 ligase and products. Incubated for 1 hr at room temperature and them left overnight in 4C. Enzymes heat-killed at 65C.<br><br>- Tom"},
| |
| | ] | | ] |
| | }); | | }); |
"
