Team:NJU-QIBEBT/humanPractice

From 2014.igem.org

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     <h1>SAFETY</h1>
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     <h1>Human Practice</h1>
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     <h2>Overview</h2>
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     <h2>1. Meetup at Huazhong Agricultural University
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     <p>We carried out our project in the M3 laboratory, Nanjing University. All of our work was completed in bio-safety level (BSL) 1 labs. Some of the parts we used in the project were extracted from E. Coli and the others were supported by the QIBEBT which posed no detriment to the researchers and environment when handled under the basic bio-safety guidelines.
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     <p>On 23rd and 24th of August, we participated in a sharing conference established by Huazhong Agricultural University. Zhou Yu, Qin Zhen, Rong Hui and Zhang Qiuhao from our team went to Wuhan and met up with students from Peking University, Sun Yat-Sen University, Wuhan University and some other universities. We gave a presentation about our project and also got to know what other teams were busy with. An internet-based group was set up for further discussion. Another team from Wuhan focuses on the fatty acid as well, thus our two teams shared experience for a mutual progress. Although some of the team’s project is quite different from ours, their experience about problems solving is still valuable to us.
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     <h2>Laboratory safety</h2>
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     <h2>2. Internship in Qingdao Institute of Bioenergy and Bioprocess Technology, CAS
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    <h3>Researcher safety
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    <p>All the experiments were performed according to the laboratory safety regulations of Nanjing University. And all the members were trained for the knowledge and skills which may be involved in the experiments to guarantee their safety and health. The cell we used in every experiment is harmless to the experimenters. EB was used in the electrophoresis, we are fully alert to it. We strictly follow the protocol and avoid careless operation.
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        In late August, 3 members of our team, Zhou Yu, Rao Bing and Zhu Ruichi, went to Qingdao Institute of Bioenergy and Bioprocess Technology for the function test of the plasmids we built. They conducted a few experiments there including extracting fatty acids and examine the species of fatty acids under the guidance of the PhD students there. One of the significant experiment they did there was they applied fermenter to cultivate a huge amount of E.coli for more quantity of fatty acids.
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     <h3>Gene safety</h3>
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    <p>There are five main genes were used in our project: AtFatA, BTE, MsbA, fasbA and fabB. First of all, we use fabA and fabB to control the saturation levels of FFAs. In fact, the two genes are the gene of E. coli , we just enhance the concentration in our engineering E.coli to produce extra products. What’s more, we use the promoter to control the gene and make sure it would not be overused. Gene MsbA is under the same regulation.
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    <h2>3. Small-scale Industrialization
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        After the experiments in Qingdao Institute of Bioenergy and Bioprocess Technology, Zhou Yu went to the factory in Qingdao with our project. He tried to apply what we studied to practice. The technician taught him some experience on fermentation. At the same time, he also learned something from the industry and managed to apply to our project. Huge fermenters were used for fermentation in a large scale.
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    <p>To produce FFA of different length, we utilized AtFatA from Arabidopsis thaliana and BTE from U. californica. Even though the two genes are transformed into our engineering E.coli from other species, there are homologous genes in every species actually. What’s more, E.coli can generate FFA naturally and the FFA is a kind of natural products in nature.
 
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     <h3>Operation safety
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     <h2>4. Establishment of iGEM Club in Nanjing University
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    <p>Every researcher must wear rubber glove and lab-gown before operating in the laboratory. Any experimental apparatus must be operated according to the safety rules. When using the UV lamp, we attach great importance to the protection on our eyes and skin.
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    <p>The operation of super clean bench:
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    <p>In order to maintain the state of sterility, anything that did not relate to the work in the super clean bench is forbidden to be put into the bench.
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    <p>Before using the super clean bench, light it with UV lamp for the purpose of sterilizing the bacteria.
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    <p>After cleaning your hand with alcohol, you must be careful for the alcohol lamp.
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    <p>When using super clean bench，the fan should keep on.
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    <h2>Public safety
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     <p>We have designed the eukaryotic circuit, which cannot be expressed by procaryotic microorganisms. And the cell we used is very fragile and it will soon die when leaving the culture medium. So it will not bring contamination to the environment.
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        A club named iGEM was formed by our team and another team ’Nanjing-China’ in our campus. We can publicize the idea of the iGEM competition to other students and thus it can attract more students to join this competition. Furthermore, this club intends to recruit some members from other departments and our team can therefore be more versatile. Anyway, the establishment of this club will enable the iGEM competition to continue at our university in the future.
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    <p>The general public has no access into our lab without permission. If they are willing to learn about our lab and our project, we will guide them to ensure their safety.
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Revision as of 12:04, 14 October 2014

Human Practice

1. Meetup at Huazhong Agricultural University

On 23rd and 24th of August, we participated in a sharing conference established by Huazhong Agricultural University. Zhou Yu, Qin Zhen, Rong Hui and Zhang Qiuhao from our team went to Wuhan and met up with students from Peking University, Sun Yat-Sen University, Wuhan University and some other universities. We gave a presentation about our project and also got to know what other teams were busy with. An internet-based group was set up for further discussion. Another team from Wuhan focuses on the fatty acid as well, thus our two teams shared experience for a mutual progress. Although some of the team’s project is quite different from ours, their experience about problems solving is still valuable to us.

2. Internship in Qingdao Institute of Bioenergy and Bioprocess Technology, CAS

In late August, 3 members of our team, Zhou Yu, Rao Bing and Zhu Ruichi, went to Qingdao Institute of Bioenergy and Bioprocess Technology for the function test of the plasmids we built. They conducted a few experiments there including extracting fatty acids and examine the species of fatty acids under the guidance of the PhD students there. One of the significant experiment they did there was they applied fermenter to cultivate a huge amount of E.coli for more quantity of fatty acids.

3. Small-scale Industrialization

After the experiments in Qingdao Institute of Bioenergy and Bioprocess Technology, Zhou Yu went to the factory in Qingdao with our project. He tried to apply what we studied to practice. The technician taught him some experience on fermentation. At the same time, he also learned something from the industry and managed to apply to our project. Huge fermenters were used for fermentation in a large scale.

4. Establishment of iGEM Club in Nanjing University

A club named iGEM was formed by our team and another team ’Nanjing-China’ in our campus. We can publicize the idea of the iGEM competition to other students and thus it can attract more students to join this competition. Furthermore, this club intends to recruit some members from other departments and our team can therefore be more versatile. Anyway, the establishment of this club will enable the iGEM competition to continue at our university in the future.

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