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Team:NCTU Formosa/Notebook/test
From 2014.igem.org
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Revision as of 11:21, 14 October 2014
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4/11
Transform of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
Transform of [Plac+PSB1A3] Ar, [mGFP+PSB1A3] Ar (G3)
Transform of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr (G1)
4/12
Cultivation of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Cultivation of [PSB1A3] Ar, [PSB1C3] Cr (G1)
Cultivation of [mGFP] Ar (G3)
4/13
Mini-prep of [mGFP] Ar, [PSB1K3] Kr (G3)
Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)
4/17
Transform of [PSB1K3] Kr (G1)
4/18
Cultivation of [PSB1K3] Kr (G1)
4/19
Transform of [Plac+PSB1A3] Ar, [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G3)
Mini-prep of [PSB1K3] Kr (G1)
4/20
Transform of [PSB1A3] Ar (G2)
Electrophoresis of [PSB1K3] Kr, [PSB1C3] Cr plasmid (G2)
4/21
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/22
Mini-prep of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/28
Electrophoresis of digested [PSB1K3] Kr(EP), [PSB1C3] Cr(EP),[PSB1A3] Ar(EP) (G1)
Cultivation of [PSB1C3] Cr (G1)
4/29
Mini-prep of [PSB1C3] Cr (G1)
Mini-prep of [PSB1C3] Cr (G2)
Transform of [B0032] Ar, and [BFP] Cr (G2)
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar (G1)
Cultivation of [B0032] Ar, and [BFP] Cr (G2)
4/30
Mini-prep of [PSB1A3] Ar (G1)
Mini-prep of [B0032] Ar, and [BFP] Cr (G2)
Transform of [B0032] Ar, and [BFP] Cr, [T7promoter] Ar Kr and [T7 polymerase] Cr (G2)
Transform of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
Digestion of [PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
Electrophoresis of plasmid and digested prouct of [PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
