"
Team:HokkaidoU Japan/Notebook/asB0034/140910 asB0034&B0034 mRFP Assay
From 2014.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
{{Team:HokkaidoU_Japan/Notebook/CSS}} | {{Team:HokkaidoU_Japan/Notebook/CSS}} | ||
<html> | <html> | ||
| + | <div id="background"> | ||
<div class="wrapper"> | <div class="wrapper"> | ||
<div id="hokkaidou-contents"> | <div id="hokkaidou-contents"> | ||
| Line 128: | Line 129: | ||
</li> | </li> | ||
</ul> | </ul> | ||
| + | </div> | ||
</div> | </div> | ||
</html> | </html> | ||
Revision as of 10:50, 14 October 2014
140910 asB0034&B0034 mRFP Assay
-
Ptrc-stem-asB0034-stem on pHN1257&Ptet-B0034-mRFP-dt on pSB6A1 on ice 30min each of 2.5µL DNA to DH5α 42C 30sec on ice 2minPtrc-stem-asB0034-stem(pHN1257)&Ptet-B0034-mRFP-dT(pSB6A1) pHN1257_up_F Tm:60.1 asB0034-R Tm:68.7Ptrc-stem-asB0034-stem(pHN1257)&Ptet-B0034-mRFP-dT(pSB6A1) LB 2 mL for 3hours Turbidity was controlled 0.1LB 2 mL IPTG 20 µ or not for 11 hours, 13hours, 15hours, 17 hoursfluoresence imageing 514 nm 610 filter
Retrieved from "http://2014.igem.org/Team:HokkaidoU_Japan/Notebook/asB0034/140910_asB0034%26B0034_mRFP_Assay"
