Team:HokkaidoU Japan/Projects/H Stem

From 2014.igem.org

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<h1 id="Overview">Overview</h1>
<h1 id="Overview">Overview</h1>
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The weak point of silencing by anti-sense RNA (asRNA) is the instability of RNA. RNA has a shorter half-life than DNA, it is essential to be stabilized for asRNA to repress mRNA sufficiently. Nakashima N and his colleagues solved this problem. They inserted each-complementary sequeces called stem regions into upstream and downstream of as RNA. These regions make asRNA form stem-loop structure, which leads to increase the stability of asRNA dramatically.
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We followed in their footsteps. We designed our unique stem sequence called H-stem and producecd a BioBricked vector for asRNA expression so that other iGEMers and researchers can easily use stemed asRNA. Using this vector. Using this vector, you can gain an asRNA-expressing construct just by inserting PCRed target gene into the vector.
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<h1 id="How_To_Use">How To Use</h1>
<h1 id="How_To_Use">How To Use</h1>
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<h1 id="Modelling">Modelling</h1>
<h1 id="Modelling">Modelling</h1>
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\[ \gamma = \frac{k_{\rm bind}c}{k_{\rm unbind}+c} \]
\[ \gamma = \frac{k_{\rm bind}c}{k_{\rm unbind}+c} \]
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Revision as of 07:46, 14 October 2014

Overview

The weak point of silencing by anti-sense RNA (asRNA) is the instability of RNA. RNA has a shorter half-life than DNA, it is essential to be stabilized for asRNA to repress mRNA sufficiently. Nakashima N and his colleagues solved this problem. They inserted each-complementary sequeces called stem regions into upstream and downstream of as RNA. These regions make asRNA form stem-loop structure, which leads to increase the stability of asRNA dramatically.

We followed in their footsteps. We designed our unique stem sequence called H-stem and producecd a BioBricked vector for asRNA expression so that other iGEMers and researchers can easily use stemed asRNA. Using this vector. Using this vector, you can gain an asRNA-expressing construct just by inserting PCRed target gene into the vector.

How To Use

Modelling

Detail
\[ X+Y \overset{k_{\rm unbind}}{\underset{k_{\rm bind}}{\rightleftharpoons}} Z \] \begin{cases} \dot{x}=a-bx-k_{\rm bind}xy+k_{\rm unbind}z & \\ \dot{y}=1-y-k_{\rm bind}xy+k_{\rm unbind}z & \\ \dot{z}=k_{\rm bind}xy-k_{\rm unbind}z-cz & \end{cases} \[ y=\frac{1}{2} \biggl\{ \sqrt{ \bigl( a-1+\frac{b}{\gamma} \bigl)^2 +4 \frac{b}{\gamma}} - \Bigl( a-1+\frac{b}{\gamma} \Bigl) \biggl\} 　\] \[ \gamma = \frac{k_{\rm bind}c}{k_{\rm unbind}+c} \]

Retrieved from "http://2014.igem.org/Team:HokkaidoU_Japan/Projects/H_Stem"