Team:Minnesota/Templates

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UMN iGEM 2014

MNtallica: Cleaning Up Heavy Metal!

tag line

Project

Mercury is a neurotoxic heavy metal with the ability to biomagnify, therefore it is a significant issue in public health and environmental studies worldwide. Its levels are continually on the rise due to copper, nickel, and gold mining activities, the industrial use of mercury catalysts, mercurial fungicides in agriculture, and the burning of fossil fuels. This has resulted in the pollution of many marine ecosystems and water reservoirs worldwide, the cleanup of which using current technology, is either not feasible or incredibly costly. This study describes the use of engineered recombinant bacteria to facilitate the biological remediation of the neurotoxin methylmercury and hazardous mercury ions from an aquatic target site into less toxic form. This synthetic microbe was incorporated in novel encapsulation technology within a cost-effective, scalable water filtering column. The employment of this device could rigorously change the practices used in mercury decontamination efforts as well as pave the way for the switch to biological rather than chemical processes. Furthermore, this technology can be applied towards bioremediation and biosensing of various other heavy metals and organic toxins in the environment.

Dry Lab

Wet Lab

Meet The Team

Policies & Practices

K-12 Education

Beginning in 2013, the UMN iGEM team launched a pilot program aimed to educate local students K-12 on topics in synthetic biology. Since the inception of the program, our outreach has been taught to several schools and over 200 students. Following the nature of an evolving educational program, our curriculum has been adapted several times to create age and time appropriate lesson plans catered to our target audience. For our most recent curriculum handbook with detailed lesson plans, click here (hyperlink to curriculum PDF).

Intellectual Property

Documentary

Public Perception

Safety in the Lab

Attributions

Wet Lab:

Mercury Project design:
Basem, Aunica,

Mercury ion Testing:
Aunica, Sarah, Cassandra, Camilo, Srijay, Jennifer, Suzie

Methylmercury testing:
Nater Lab, Aunica, Nicholas, Srijay, Patrick, Suzie, Basem

Cadmium, Zinc, Copper project design:
Basem, Stephen, Aunica

Cadmium, Zinc, Copper Testing:
Aunica, Cassandra, Jessica ???

Kill Switch Proposal:
David, Sarah, Camilo, Stephen, Basem

pDU1358 received from Dr. Anne O. Summers, University of Georgia
pSB74 received through addgene from Keasling Lab

Composite parts:
mer operon:
Primer design: Basem, Stephen
Parts cloning: Basem, Jennifer, Stephen, Valeriu

phsABC:
Primer design: Basem, Stephen
Parts cloning: Basem, Stephen, Valeriu

Single parts:
merR:
Primer design: Basem, Stephen
Parts cloning: Cassandra
merT:
Primer design: Stephen, Basem
Parts cloning: Sarah, Jennifer
merP:
Primer design: Basem, Stephen
Parts cloning: Camilo, Logan
merA:
Primer design: Stephen, Basem
Parts cloning: Valeriu, Jessica
Characterization: Cassandra, Sarah
merB:
Primer design: Basem, Stephen
Parts cloning: Logan, David

Chassis Transformations:
Pseudomonas putida: Basem
Shewanella oneidensis: Basem
E. coli K12: Basem, David

Rhodopseudomonas
Project design: Basem, Stephen
Parts cloning: Stephen, Basem

Dry lab:

EncapsuLab:
Protocol Design: Srijay, Patrick, David, Nicholas
Cell encapsulation: Nicholas, Patrick, Srijay, David, Basem, Suzie
Cell Viability Testing: Patrick, David, Nicholas
SEM encapsulation imaging: Nicholas, UofM imaging center
Device design: Roxana, Nicholas
Mathematical modelling: Di, Zhiyi, Patrick, David

Policies and Practices:

Outreach, presentations, public perception studies

School Curriculum design: Basem, Suzie
Science Museum Curriculum Design: everybody
Middle School Classroom outreach: Jess, Basem, Cassandra, Jennifer, Suzie
Science Museum outreach: Jess, Jen, David, Sarah, Cassandra, Basem, Srijay, Di, Holly, Logan
3M presentation: Suzie, Basem, Cassandra, Stephen, Jess
Cargill presentation: ???
State Fair outreach:
tabling & survey: Cassandra Taylor Jess Jen Basem Suzie Nicholas Stephen Roxana Di
Srijay Patrick Holly Logan
Survey statistics: Taylor
slideshow: Jess, David, Logan
giveaways: CBS, local businesses gift cards, Rob Rakow
survey content: everyone
State Fair game show presentation: Cassandra, Taylor

Ethics :
blog: Basem, Cassandra, Logan, Jen
Documentary: Jennifer, David, Colombia iGEM team

Business Plan:
Justin, Tanner, Basem, Tamara, ?

Economic Analysis: ??? + IP team at OTC

Colombia collaboration: (magnetic stirrer) Stephen
Other collaborations??

Wiki development
Design: Mari, Chris, Aaron, Basem, ??
Icons, figures development: Mari, Basem, Nicholas, ???
Coding, CSS, javascript: Aaron, Chris,
Lab notebook: Sarah Lucas

Poster:
Basem, ??

Team Logo
Nicholas

Administrative forms, IP, safety:
Basem

Parts Submission form & shipping
Stephen

Public relations and team contact
Basem, Jessica

Grant writing, fundraising
Basem, Jess, David, Cassandra

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 		<div class="slide" id="slide1" data-anchor="slide1">
 			<h4>Policies & Practices</h4>
 		</div>
 		<div class="slide" id="slide2" data-anchor="slide2">
 			<h4>K-12 Education</h4>
+            <h3> Beginning in 2013, the UMN iGEM team launched a pilot program aimed to educate local students K-12 on topics in synthetic biology. Since the inception of the program, our outreach has been taught to several schools and over 200 students. Following the nature of an evolving educational program, our curriculum has been adapted several times to create age and time appropriate lesson plans catered to our target audience. For our most recent curriculum handbook with detailed lesson plans, click here (hyperlink to curriculum PDF).
+ </h3>
 		</div>