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Team:Paris Saclay/Notebook/September/16
From 2014.igem.org
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well 2-9 = chromo yellow in topo plasmid | well 2-9 = chromo yellow in topo plasmid | ||
well 10-17 = chromo blue in topo plasmid | well 10-17 = chromo blue in topo plasmid | ||
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| + | ==Photo of the Day== | ||
| + | [[File:Paris Saclay 2_september.jpg|300px|center]] | ||
| + | |||
| + | Work of [http://ateliers-artistes-belleville.fr/artiste/therese-bichon/ Thérèse Bichon] , one of those few we have interviewed during this month of september. | ||
| + | |||
| + | To learn more about the ethic aspect of our project, just click [https://2014.igem.org/Team:Paris_Saclay/Ethics here] | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 06:46, 14 October 2014
Contents |
Lab Work
By Mélanie
D- Lemon scent
Plasmids extraction
Extraction of the plasmid from the culture made yesterday(PS)
Digestion of PS topo plasmids
by PacI and PSTI
(well 2 and 3 : PacI digestion)
Ligation
After digestion, ligation overnight in PPS1
PCR Colony
Test of the transformation made yesterday to know if we had obtained a plasmid]
primer to ckeck the direction of the insert PPS2 : iPS72-96
PPS4 : ips72-97
= no insert (just well 1-9)
liquide culture
liquide culture from yesterday's colony to make stock tomorrow
PCR cad to cloning in pSBIC3
PCR and digestion PSTI/ecoRI and ligation
PCR GS
PCR to check if we have the insert in our topo plasmid
B- Construction of the fusion protein (color)
PCR
PCR to check if we have the insert in our topo plasmid
well 2-9 = chromo yellow in topo plasmid well 10-17 = chromo blue in topo plasmid
Photo of the Day
Work of [http://ateliers-artistes-belleville.fr/artiste/therese-bichon/ Thérèse Bichon] , one of those few we have interviewed during this month of september.
To learn more about the ethic aspect of our project, just click here
