Revision as of 05:36, 14 October 2014

Protocols / SDS-PAGE

Material:
12 % Running gel	2.1 ml H2O, 2.5 ml acrylamide 4K 29:1 mix (40 %), 1.6 ml 1.5 M Tris-HCl pH 8.8, 62.5 μl 10 % SDS, 62.5 μl 10 % APS, 2.5 μl TEMED

12 % Stacking gel	2.7 ml H2O, 680 μl acrylamide 4K 29:1 mix (40 %), 540 μl 1 M Tris-HCl pH 6.8, 40 μl 10 % SDS, 30 μl 10 % APS, 8 μl TEMED
Electrophoresis system

Running buffer	250 mM Tris-HCl pH 8.3, 1.92 mM glycine, 0,1 % (v/v) SDS

@@ Line 8: / Line 8: @@
 <table colspan="2"><tr><td><h4>Material:</h4></td>
-<tr><td>12 % Running gel</td><td>2.1 ml  H2O,<br>2.5 ml  acrylamide 4K 29:1 mix  (40 %),<br> 1.6 ml 1.5 M Tris-HCl pH 8.8,<br> 62.5 μl 10 % SDS,<br> 62.5 μl 10 % APS,<br> 2.5 μl TEMED</td></tr><tr><td>12 % Stacking gel</td><td>2.7 ml   H2O,<br>680 μl  acrylamide 4K  29:1 mix  (40 %),<br>540 μl 1 M Tris-HCl pH 6.8,<br>40 μl 10 % SDS,<br>30 μl 10 % APS,<br>8 μl TEMED</td></tr><tr><td>Electrophoresis system</td><td></td></tr><tr><td></td><td></td></tr><tr><td>Running buffer</td><td>250 mM Tris-HCl pH 8.3,<br>1.92 mM glycine,<br> 0,1 % (v/v) SDS</td></tr></table>
+<tr><td>12 % Running gel</td><td>2.1 ml  H2O,<br>2.5 ml  acrylamide 4K 29:1 mix  (40 %),<br> 1.6 ml 1.5 M Tris-HCl pH 8.8,<br> 62.5 μl 10 % SDS,<br> 62.5 μl 10 % APS,<br> 2.5 μl TEMED</td></tr><tr><td></td><td></td></tr><tr><td>12 % Stacking gel</td><td>2.7 ml   H2O,<br>680 μl  acrylamide 4K  29:1 mix  (40 %),<br>540 μl 1 M Tris-HCl pH 6.8,<br>40 μl 10 % SDS,<br>30 μl 10 % APS,<br>8 μl TEMED</td></tr><tr><td>Electrophoresis system</td><td></td></tr><tr><td></td><td></td></tr><tr><td>Running buffer</td><td>250 mM Tris-HCl pH 8.3,<br>1.92 mM glycine,<br> 0,1 % (v/v) SDS</td></tr></table>
 <p id="Fusszeile"><br><br><br><br><img src="https://static.igem.org/mediawiki/2014/6/6a/Hannover_20141010Fusszeile2.jpg" width="980px" style="float:right" /></p>
 </body>
 </html>