The goal of this subproject is to engineer <i>Gluconacetobacter hansenii,</i> which produces large quantities of bacterial cellulose (BC), to use the acetylation machinery found in the wrinkly spreader isolate SBW25 of <i>Pseudomonas fluorescens</i> to produce bacterial cellulose acetate (BCOAc) towards its ultimate application as the foundation of a fully biological UAV. Industrially-produced cellulose acetate has many uses as a synthetic fiber and has advantages over pure BC in terms of material properties. However, its production presents some issues in that it requires harsh chemical processes, making the biological manufacturing method highly valuable. Using directed evolution, we plan to select for those organisms which produce the polymer with highest acetate content. In addition, we seek to create a fusion protein which will have the capacity to both cross-link BCOAc polymers (improving material properties) and allow the modular addition of any cell (e.g. a biosensor). This will be accomplished through the expression of a biotinylated membrane protein, through the protein’s streptavidin domain, making the UAV highly programmable.
+
The goal of this subproject is to create a cellulose cross-linking protein to increase material strength and allow for the modular attachment of biological sensors. This fusion protein contains two distinct cellulose-binding domains on either side of a streptavidin domain. The cellulose-binding domains cross link the cellulose fibers while the streptavidin serves as a binding domain for biological sensors. Streptavidin has a strong affinity for biotin thus, a cell expressing an outer membrane protein that has been biotinylated will bind tightly to this domain. This will allow our UAV to make use of a number of biological sensors.
The goal of this subproject is to create a cellulose cross-linking protein to increase material strength and allow for the modular attachment of biological sensors. This fusion protein contains two distinct cellulose-binding domains on either side of a streptavidin domain. The cellulose-binding domains cross link the cellulose fibers while the streptavidin serves as a binding domain for biological sensors. Streptavidin has a strong affinity for biotin thus, a cell expressing an outer membrane protein that has been biotinylated will bind tightly to this domain. This will allow our UAV to make use of a number of biological sensors.
Image description goes here.
Image description goes here.
Image description goes here.
Image description goes here.
Image description goes here.
Image description goes here.
Results
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Cras venenatis lorem et odio sodales, vitae aliquet nunc ultrices. Nunc lacinia nulla urna, sed aliquam nisl fermentum sed. Phasellus vel pellentesque tortor, in tincidunt metus. Aliquam ac laoreet risus. Fusce venenatis, justo id luctus dictum, turpis libero tincidunt mauris, sit amet tempor lectus tortor ut ante. Pellentesque egestas felis et est venenatis, eget lobortis dui adipiscing. Suspendisse volutpat sem eu ornare tincidunt. Mauris pharetra sed justo vitae sodales. Nulla in sodales tortor, placerat tempor dui.
Methods & Safety
Fusce venenatis, justo id luctus dictum, turpis libero tincidunt mauris, sit amet tempor lectus tortor ut ante. Pellentesque egestas felis et est venenatis, eget lobortis dui adipiscing. Suspendisse volutpat sem eu ornare tincidunt. Mauris pharetra sed justo vitae sodales. Nulla in sodales tortor, placerat tempor dui.
"
