Team:Oxford/Events
From 2014.igem.org
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<h1 class='modal-heading'>Synthetic Biology and Restriction Enzymes Talk @ Question of Taste Workshop</h1> | <h1 class='modal-heading'>Synthetic Biology and Restriction Enzymes Talk @ Question of Taste Workshop</h1> | ||
- | <h2 class='modal-heading'>Audience:</h2> 15-20 A2-level Biology students (17-18 years old) and their teachers.<br> | + | <h2 class='modal-heading'>Audience:</h2> 15-20 A2-level Biology students (17-18 years old) and their teachers.<br><br> |
- | <h2 class='modal-heading'>The Brief:</h2> Give 20-25minute presentation about synthetic biology focusing on molecular biology techniques during the University Museum of Natural History ‘A Question of Taste’ day-long workshop.<br> | + | <h2 class='modal-heading'>The Brief:</h2> Give 20-25minute presentation about synthetic biology focusing on molecular biology techniques during the University Museum of Natural History ‘A Question of Taste’ day-long workshop.<br><br> |
<h2 class='modal-heading'>Content:</h2>In the course of the day the students undertook PCR, restriction enzyme digest and gel electrophoresis so we discussed how these techniques could be used in synthetic biology.<BR><BR> | <h2 class='modal-heading'>Content:</h2>In the course of the day the students undertook PCR, restriction enzyme digest and gel electrophoresis so we discussed how these techniques could be used in synthetic biology.<BR><BR> | ||
PUT PRESENTATION HERE!<BR><BR> | PUT PRESENTATION HERE!<BR><BR> | ||
- | We also developed a model of a plasmid and insert to demonstrate complementary ends and the problems with a one enzyme restriction digest and how this can be overcome by a double restriction enzyme digest.<br> | + | We also developed a model of a plasmid and insert to demonstrate complementary ends and the problems with a one enzyme restriction digest and how this can be overcome by a double restriction enzyme digest.<br><br> |
<h2 class='modal-heading'>How were we informed by the survey and focus groups?</h2> | <h2 class='modal-heading'>How were we informed by the survey and focus groups?</h2> | ||
- Addressing the concern raised in our focus group that the possibilities presented about synthetic biology are ‘over-hyped’ we only gave examples of synthetic biology projects that have been successfully undertaken. | - Addressing the concern raised in our focus group that the possibilities presented about synthetic biology are ‘over-hyped’ we only gave examples of synthetic biology projects that have been successfully undertaken. | ||
- | - Included discussion as to why we use antibiotic resistance genes (which in one session lead to a discussion of alternative selectable markers).<br> | + | - Included discussion as to why we use antibiotic resistance genes (which in one session lead to a discussion of alternative selectable markers).<br><br> |
<h2 class='modal-heading'>What did we improve for our second go?</h2> | <h2 class='modal-heading'>What did we improve for our second go?</h2> | ||
The second time round added in an animation to show how restriction enzyme sites can be added to a fragment of DNA by PCR. | The second time round added in an animation to show how restriction enzyme sites can be added to a fragment of DNA by PCR. |
Revision as of 22:32, 13 October 2014