"
Team:NCTU Formosa/Notebook/test2
From 2014.igem.org
| Line 62: | Line 62: | ||
5/1 | 5/1 | ||
</p> | </p> | ||
| - | <p>Cultivation of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)</p> | + | <p><font color="yellow">Cultivation of</font> [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)</p> |
| - | <p>Transform of [T7 polymerase]Cr (G2)</p> | + | <p><font color="yellow">Transform of</font> [T7 polymerase]Cr (G2)</p> |
<p>5/2</p> | <p>5/2</p> | ||
| - | <p>Cultivation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)</p> | + | <p><font color="yellow">Cultivation of</font> [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)</p> |
| - | <p>Mini-prep of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)</p> | + | <p><font color="yellow">Mini-prep of</font> [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)</p> |
| - | <p>Digestion of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and [B0015]Ar*2(ES & XP) (G2)</p> | + | <p><font color="yellow">Digestion of</font> [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and [B0015]Ar*2(ES & XP) (G2)</p> |
<p>5/3</p> | <p>5/3</p> | ||
| - | <p>Mini-prep of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)</p> | + | <p><font color="yellow">Mini-prep of</font> [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)</p> |
| - | <p>Mini-pep of [B0032] Ar, [B0015] Ar,[BFP] Cr (G3)</p> | + | <p><font color="yellow">Mini-pep of</font> [B0032] Ar, [B0015] Ar,[BFP] Cr (G3)</p> |
| - | <p>Digestion of [J23101] Ar (ES),[K592100]Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3] Cr(EP),[PSB1K3] Kr(EP) mini (G1)</p> | + | <p><font color="yellow">Digestion of</font> [J23101] Ar (ES),[K592100]Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3] Cr(EP),[PSB1K3] Kr(EP) mini (G1)</p> |
| - | <p>Electrophoresis of plasmid and digested product of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and B0015*2(ES & XP) (G2)</p> | + | <p><font color="yellow">Electrophoresis of plasmid and digested product of</font> [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and B0015*2(ES & XP) (G2)</p> |
| - | <p>Electrophoresis of digested product of [J23101] Ar (ES),[K592100] Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3]Cr(EP),[PSB1K3] Kr(EP) (G1)</p> | + | <p><font color="yellow">Electrophoresis of</font> digested product of [J23101] Ar (ES),[K592100] Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3]Cr(EP),[PSB1K3] Kr(EP) (G1)</p> |
| - | <p>Cultivation of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)</p> | + | <p><font color="yellow">Cultivation of</font> [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)</p> |
| - | <p>Ligation | + | <p><font color="yellow">Ligation of</font> [BFP+B0032+PSB1K3]Kr (G2)</p> |
| - | <p>Ligation of [J23101+B0032+PSB1C3] Cr (G1)</p> | + | <p><font color="yellow">Ligation of</font> [J23101+B0032+PSB1C3] Cr (G1)</p> |
| - | <p>Transform of ligation product [BFP+B0032+PSB1K3]Kr (G2)</p> | + | <p><font color="yellow">Transform of</font> ligation product [BFP+B0032+PSB1K3]Kr (G2)</p> |
| - | <p>Transform of [T7polymerase]Cr and [B0015]Ar (G2)</p></div> | + | <p><font color="yellow">Transform of</font> [T7polymerase]Cr and [B0015]Ar (G2)</p></div> |
<div class="single note"> | <div class="single note"> | ||
| Line 87: | Line 87: | ||
<p>5/4<p> | <p>5/4<p> | ||
| - | <p>Mini-prep of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)</p> | + | <p><font color="yellow">Mini-prep of</font> [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)</p> |
| - | <p>Mini-prep of [B0015*3] Ar and [T7polymerase] Cr (G2)</p> | + | <p><font color="yellow">Mini-prep of</font> [B0015*3] Ar and [T7polymerase] Cr (G2)</p> |
| - | <p>Digestion of [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP)(G1)</p> | + | <p><font color="yellow">Digestion of</font> [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP)(G1)</p> |
| - | <p>Digestion of [B0015*3] Ar and [T7polymerase] Cr (G2)</p> | + | <p><font color="yellow">Digestion of</font> [B0015*3] Ar and [T7polymerase] Cr (G2)</p> |
| - | <p>Digestion of [B0015] Ar(XP), [BFP] Cr(XP), [PSB1A3] Ar(EP), [PSB1C3] Cr(EP), [B0032] Ar (XP) (G3)</p> | + | <p><font color="yellow">Digestion of</font> [B0015] Ar(XP), [BFP] Cr(XP), [PSB1A3] Ar(EP), [PSB1C3] Cr(EP), [B0032] Ar (XP) (G3)</p> |
| - | <p>< | + | <p><font color="yellow">Transform of</font> [Pcons promoter] Ar, [RBS+BFP] Kr and [T7 promoter] Kr (G2)</p> |
| - | <p>Transform of [LacI] Ar (G3)</p> | + | <p><font color="yellow">Transform of</font> [LacI] Ar (G3)</p> |
| - | <p>Electrophoresis of plasmid and digested product of [B0015*3] Ar and [T7polymerase]Cr (G2)</p> | + | <p><font color="yellow">Electrophoresis of</font> plasmid and digested product of [B0015*3] Ar and [T7polymerase]Cr (G2)</p> |
| - | <p>Electrophoresis of digested product of [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP), [K592100] Cr (ES)(G1)</p> | + | <p><font color="yellow">Electrophoresis of digested product of</font> [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP), [K592100] Cr (ES)(G1)</p> |
| - | <p>Cultivation of [B0015] Ar, [PSB1A3] Ar(G1)</p> | + | <p><font color="yellow">Cultivation of</font> [B0015] Ar, [PSB1A3] Ar(G1)</p> |
| - | <p>Cultivation of [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar (G3)</p> | + | <p><font color="yellow">Cultivation of</font> [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar (G3)</p> |
<p>5/5</p> | <p>5/5</p> | ||
| - | <p>Mini-prep of [PSB1A3] Ar,[B0015]Ar (G1)</p> | + | <p><font color="yellow">Mini-prep of</font> [PSB1A3] Ar,[B0015]Ar (G1)</p> |
| - | <p>Mini-prep of [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar (G3)</p> | + | <p><font color="yellow">Mini-prep of</font> [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar (G3)</p> |
| - | <p>Digestion of [PSB1A3] Ar(EP),[B0015]Ar(XP)(G1)</p> | + | <p><font color="yellow">Digestion of</font> [PSB1A3] Ar(EP),[B0015]Ar(XP)(G1)</p> |
| - | <p>Electrophoresis of digested product [PSB1A3] Ar(EP),[B0015]Ar(XP),[PSB1K3]Kr(EP) (G1)</p> | + | <p><font color="yellow">Electrophoresis of digested product</font> [PSB1A3] Ar(EP),[B0015]Ar(XP),[PSB1K3]Kr(EP) (G1)</p> |
<p> | <p> | ||
5/6 | 5/6 | ||
| - | Mini-prep of [LacI] Ar (G3) | + | <font color="yellow">Mini-prep of</font> [LacI] Ar (G3) |
</p> | </p> | ||
<p> | <p> | ||
5/7 | 5/7 | ||
| - | Transform of [PT7polymerase]Cr and [J61048]Ar (G2) | + | <font color="yellow">Transform of</font> [PT7polymerase]Cr and [J61048]Ar (G2) |
</p><p> | </p><p> | ||
5/8 | 5/8 | ||
| - | Transform of [B0015*3] Ar and [J61048*2] Ar (G2) | + | <font color="yellow">Transform of</font> [B0015*3] Ar and [J61048*2] Ar (G2) |
| - | Transform of [B0015] Ar and [J61048] Ar (G3) | + | <font color="yellow">Transform of</font> [B0015] Ar and [J61048] Ar (G3) |
| - | Transform of [B0015]Ar,[J61048]Ar,[J23101]Ar (G1) | + | <font color="yellow">Transform of</font> [B0015]Ar,[J61048]Ar,[J23101]Ar (G1) |
| - | Mini-prep of [PT7polymerase]Cr, [T7polymerase*2]Cr and [B0015*2]Ar (G2) | + | <font color="yellow">Mini-prep of</font> [PT7polymerase]Cr, [T7polymerase*2]Cr and [B0015*2]Ar (G2) |
| - | Digestion of [T7polymerase] Kr (EP) and [B0015] Ar (ES) (G2) | + | <font color="yellow">Digestion of</font> [T7polymerase] Kr (EP) and [B0015] Ar (ES) (G2) |
| - | Digestion of [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3) | + | <font color="yellow">Digestion of</font> [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3) |
| - | Electrophoresis of digested product of [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3) | + | <font color="yellow">Electrophoresis of</font> digested product of [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3) |
</p><p> | </p><p> | ||
5/9 | 5/9 | ||
| - | Cultivation of [B0015] Ar, [J61048] Ar [LacI] Ar (G3) | + | <font color="yellow">Cultivation of</font> [B0015] Ar, [J61048] Ar [LacI] Ar (G3) |
</p> | </p> | ||
<p> | <p> | ||
5/10 | 5/10 | ||
| - | Mini-prep [LacI] Ar (G3) | + | <font color="yellow">Mini-prep of</font> [LacI] Ar (G3) |
</p> | </p> | ||
| Line 136: | Line 136: | ||
<div class="single note"> | <div class="single note"> | ||
<p>5/11</p><p> | <p>5/11</p><p> | ||
| - | Cultivation of [J61048] Ar (G2) (ES)</p><p> | + | <font color="yellow">Cultivation of</font> [J61048] Ar (G2) (ES)</p><p> |
| - | Electrophoresis of plasmid and digested product of [B0015]Ar(ES), [T7polymerase] Kr(only mini) and [T7promoter] Kr (EP)</p><p> | + | <font color="yellow">Electrophoresis of</font> plasmid and digested product of [B0015]Ar(ES), [T7polymerase] Kr(only mini) and [T7promoter] Kr (EP)</p><p> |
| - | Transform of [T7 promoter] Kr (G2)</p><p> | + | <font color="yellow">Transform of</font> [T7 promoter] Kr (G2)</p><p> |
</p> | </p> | ||
<p> | <p> | ||
5/12 | 5/12 | ||
| - | Digestion of [T7polymerase] Kr (ES) and [J61048] Ar (ES) (G2)</p><p> | + | <font color="yellow">Digestion of</font> [T7polymerase] Kr (ES) and [J61048] Ar (ES) (G2)</p><p> |
</p> | </p> | ||
<p> | <p> | ||
5/13 | 5/13 | ||
| - | Transform of [T7 promoter] Kr (G2)</p><p> | + | <font color="yellow">Transform of</font> [T7 promoter] Kr (G2)</p><p> |
</p> | </p> | ||
<p> | <p> | ||
5/15 | 5/15 | ||
| - | Electrophoresis of plasmid and digested product of [J61048]Ar (ES), [T7polymerase*3]Kr(ES & XP) and [PSBIK3]Kr(EP) (G2)</p><p> | + | <font color="yellow">Electrophoresis of plasmid and digested product of</font> [J61048]Ar (ES), [T7polymerase*3]Kr(ES & XP) and [PSBIK3]Kr(EP) (G2)</p><p> |
| - | Cultivation of [B0015]Ar, [J61048]Ar, [J23101]Ar, [PSB1K3]Kr (G1)</p><p> | + | <font color="yellow">Cultivation of</font> [B0015]Ar, [J61048]Ar, [J23101]Ar, [PSB1K3]Kr (G1)</p><p> |
| - | Mini-prep of [PSB1K3]Kr (G1)</p><p> | + | <font color="yellow">Mini-prep of</font> [PSB1K3]Kr (G1)</p><p> |
| - | Digestion of [PSB1K3]Kr (EP) (G1)</p><p> | + | <font color="yellow">Digestion of</font> [PSB1K3]Kr (EP) (G1)</p><p> |
</p> | </p> | ||
| Line 163: | Line 163: | ||
<p> | <p> | ||
5/19</p> | 5/19</p> | ||
| - | <p>Cultivation of [J23101]Ar,[B0015]Ar,[J61048]Ar (G1) | + | <p><font color="yellow">Cultivation of</font> [J23101]Ar,[B0015]Ar,[J61048]Ar (G1) |
</p><p> | </p><p> | ||
5/20</p><p> | 5/20</p><p> | ||
| - | Transform of [T7polymerase] Kr and [PSB1K3] Kr (G2)</p><p> | + | <font color="yellow">Transform of</font> [T7polymerase] Kr and [PSB1K3] Kr (G2)</p><p> |
| - | Electrophoresis of plasmid and digested product of [T7polymerase*6] Kr and [PSB1K3*4]Kr (G2)</p><p> | + | <font color="yellow">Electrophoresis of</font> plasmid and digested product of [T7polymerase*6] Kr and [PSB1K3*4]Kr (G2)</p><p> |
| - | Mini-prep of [J23101]Ar, [B0015]Ar, [J61048]Ar (G1)</p><p> | + | <font color="yellow">Mini-prep of</font> [J23101]Ar, [B0015]Ar, [J61048]Ar (G1)</p><p> |
| - | Digestion of [J23101]Ar (ES), [B0015]Ar (XP), [J61048]Ar (XP) (G1)</p><p> | + | <font color="yellow">Digestion of</font> [J23101]Ar (ES), [B0015]Ar (XP), [J61048]Ar (XP) (G1)</p><p> |
</p> | </p> | ||
<p> | <p> | ||
5/21</p> | 5/21</p> | ||
<p> | <p> | ||
| - | Digestion of [T7polymerase*6] Kr and [PSB1K3*2] Kr (G2)</p><p> | + | <font color="yellow">Digestion of</font> [T7polymerase*6] Kr and [PSB1K3*2] Kr (G2)</p><p> |
| - | Mini-prep of [T7polymerase] Kr and [PSB1K3] Kr (G2)</p><p> | + | <font color="yellow">Mini-prep of</font> [T7polymerase] Kr and [PSB1K3] Kr (G2)</p><p> |
| - | Electrophoresis of digested product and plasmid [J23101]Ar(ES), [B0015]Ar(XP), [J61048]Ar(XP) and digested product [PSB1K3]Kr(EP) (G1)</p><p> | + | <font color="yellow">Electrophoresis of digested product and plasmid</font> [J23101]Ar(ES), [B0015]Ar(XP), [J61048]Ar(XP) and digested product [PSB1K3]Kr(EP) (G1)</p><p> |
| - | Ligation of [K592100+J61048+PSB1K3] Kr (G1)</p><p> | + | <font color="yellow">Ligation of</font> [K592100+J61048+PSB1K3] Kr (G1)</p><p> |
</p> | </p> | ||
<p> | <p> | ||
5/22 | 5/22 | ||
</p> | </p> | ||
| - | <p>Electrophoresis of plasmid and digested product of [T7polymerase] Kr (ES) and [PSB1K3]Kr (EP) (G2) | + | <p><font color="yellow">Electrophoresis of plasmid and digested product of</font> [T7polymerase] Kr (ES) and [PSB1K3]Kr (EP) (G2) |
</p> | </p> | ||
<p> | <p> | ||
| Line 188: | Line 188: | ||
</p> | </p> | ||
<p> | <p> | ||
| - | Transform of ligation product [K592100+J61048+PSB1K3]Kr (G1)</p><p> | + | <font color="yellow">Transform of</font> ligation product [K592100+J61048+PSB1K3]Kr (G1)</p><p> |
</p> | </p> | ||
<p> | <p> | ||
| Line 194: | Line 194: | ||
</p> | </p> | ||
<p> | <p> | ||
| - | Digestion of [J61048] Ar (XP) (G2)</p><p> | + | <font color="yellow">Digestion of</font> [J61048] Ar (XP) (G2)</p><p> |
| - | PCR | + | <font color="yellow">PCR of</font> [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr (G2)</p><p> |
| - | Electrophoresis of PCR products [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr, [J61048] Ar (G2)</p><p> | + | <font color="yellow">Electrophoresis of PCR products</font> [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr, [J61048] Ar (G2)</p><p> |
</p> | </p> | ||
| Line 205: | Line 205: | ||
</p> | </p> | ||
<p> | <p> | ||
| - | Electrophoresis of digested product of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3) | + | <font color="yellow">Electrophoresis of digested product of</font> [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3) |
</p> | </p> | ||
<p> | <p> | ||
| - | Digestion of [J61048] Ar (XP) and [T7polymerase] Kr (ES) (G2) | + | <font color="yellow">Digestion of</font> [J61048] Ar (XP) and [T7polymerase] Kr (ES) (G2) |
</p> | </p> | ||
<p> | <p> | ||
| - | Cultivation of [PSB1K3*3] Kr(G2) | + | <font color="yellow">Cultivation of</font> [PSB1K3*3] Kr(G2) |
</p> | </p> | ||
<p> | <p> | ||
| Line 217: | Line 217: | ||
</p> | </p> | ||
<p> | <p> | ||
| - | PCR | + | <font color="yellow">PCR of</font> [PSBIK3*5]Kr , [PSBIA3*5]Ar , [PSBIC3*5]Cr ,[J61048*5]Ar ,[B0015*5]Ar and [BFP*5](G1) |
</p> | </p> | ||
<p> | <p> | ||
| - | Mini-prep of [PSB1K3*3] Kr(G2) | + | <font color="yellow">Mini-prep of</font> [PSB1K3*3] Kr(G2) |
</p> | </p> | ||
<p> | <p> | ||
| - | Digestion of [PSB1K3*3] Kr (EP) (G2) | + | <font color="yellow">Digestion of</font> [PSB1K3*3] Kr (EP) (G2) |
</p> | </p> | ||
<p> | <p> | ||
| - | Electrophoresis of plasmid and digested [PSB1K3] Kr (EP), [T7polymerase] Kr (ES), [J61048] Ar (XP) (G2) | + | <font color="yellow">Electrophoresis of plasmid and digested product of</font> [PSB1K3] Kr (EP), [T7polymerase] Kr (ES), [J61048] Ar (XP) (G2) |
</p> | </p> | ||
<p> | <p> | ||
| Line 232: | Line 232: | ||
</p> | </p> | ||
<p> | <p> | ||
| - | Digestion of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3) | + | <font color="yellow">Digestion of </font>[mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3) |
</p> | </p> | ||
<p> | <p> | ||
| - | Transform of [J23101] Ar (G1) | + | <font color="yellow">Transform of </font>[J23101] Ar (G1) |
</p> | </p> | ||
<p> | <p> | ||
| - | Transform of [T7 promoter] Ar Kr (G2) | + | <font color="yellow">Transform of </font>[T7 promoter] Ar Kr (G2) |
</p> | </p> | ||
<p> | <p> | ||
| - | Electrophoresis of plasmid and digested product of [J61048] Ar (ES), [T7polymerase] Kr (ES) (G2) | + | <font color="yellow">Electrophoresis of plasmid and digested product of</font> [J61048] Ar (ES), [T7polymerase] Kr (ES) (G2) |
</p> | </p> | ||
<p> | <p> | ||
| - | Ligation of [J61048+J61048] Cr, [T7polymerase+J61048] Kr, [RBS+BFP] Kr (G2) | + | <font color="yellow">Ligation of</font> [J61048+J61048] Cr, [T7polymerase+J61048] Kr, [RBS+BFP] Kr (G2) |
</p> | </p> | ||
<p> | <p> | ||
| - | Ligation of | + | <font color="yellow">Ligation of </font> [Plac+B0032+PSB1K3] Kr (G3) |
</p> | </p> | ||
<p> | <p> | ||
| Line 253: | Line 253: | ||
</p> | </p> | ||
<p> | <p> | ||
| - | Transform of ligation product (G2) | + | <font color="yellow">Transform of</font> ligation product (G2) |
</p> | </p> | ||
<p> | <p> | ||
| - | Transform of ligation product [Plac+B0032+PSB1K3] Kr (G3) | + | <font color="yellow">Transform of</font> ligation product [Plac+B0032+PSB1K3] Kr (G3) |
</p> | </p> | ||
<br></div> | <br></div> | ||
Revision as of 16:15, 13 October 2014
- Sunday
- Monday
- Tuesday
- Wednesday
- Thursday
- Friday
- Saturday
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8
- 9
- 10
- 11
- 12
- 13
- 14
- 15
- 16
- 17
- 18
- 19
- 20
- 21
- 22
- 23
- 24
- 25
- 26
- 27
- 28
- 29
- 30
- 31
5/1
Cultivation of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)
Transform of [T7 polymerase]Cr (G2)
5/2
Cultivation of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
Mini-prep of [PSB1C3]Cr, [BFP]Cr, [T7promoter]ArKr, [T7 polymerase]Cr and [B0015]Ar (G2)
Digestion of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and [B0015]Ar*2(ES & XP) (G2)
5/3
Mini-prep of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
Mini-pep of [B0032] Ar, [B0015] Ar,[BFP] Cr (G3)
Digestion of [J23101] Ar (ES),[K592100]Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3] Cr(EP),[PSB1K3] Kr(EP) mini (G1)
Electrophoresis of plasmid and digested product of [PSB1C3]Cr(EP), [BFP]Cr(XP), [T7 polymerase]Cr(ES), [T7promoter]ArKr(ES) and B0015*2(ES & XP) (G2)
Electrophoresis of digested product of [J23101] Ar (ES),[K592100] Cr(ES),[B0032] Ar(XP),[B0015] Ar(XP),[PSB1A3] Ar(EP),[PSB1C3]Cr(EP),[PSB1K3] Kr(EP) (G1)
Cultivation of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)
Ligation of [BFP+B0032+PSB1K3]Kr (G2)
Ligation of [J23101+B0032+PSB1C3] Cr (G1)
Transform of ligation product [BFP+B0032+PSB1K3]Kr (G2)
Transform of [T7polymerase]Cr and [B0015]Ar (G2)
5/4
Mini-prep of [B0015] Ar, [PSB1A3] Ar, [PSB1K3] Kr (G1)
Mini-prep of [B0015*3] Ar and [T7polymerase] Cr (G2)
Digestion of [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP)(G1)
Digestion of [B0015*3] Ar and [T7polymerase] Cr (G2)
Digestion of [B0015] Ar(XP), [BFP] Cr(XP), [PSB1A3] Ar(EP), [PSB1C3] Cr(EP), [B0032] Ar (XP) (G3)
Transform of [Pcons promoter] Ar, [RBS+BFP] Kr and [T7 promoter] Kr (G2)
Transform of [LacI] Ar (G3)
Electrophoresis of plasmid and digested product of [B0015*3] Ar and [T7polymerase]Cr (G2)
Electrophoresis of digested product of [B0015] Ar(XP), [PSB1A3] Ar(EP), [PSB1K3] Kr (EP), [K592100] Cr (ES)(G1)
Cultivation of [B0015] Ar, [PSB1A3] Ar(G1)
Cultivation of [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar (G3)
5/5
Mini-prep of [PSB1A3] Ar,[B0015]Ar (G1)
Mini-prep of [Plac] Ar, [PSB1C3] Cr, [mGFP] Ar (G3)
Digestion of [PSB1A3] Ar(EP),[B0015]Ar(XP)(G1)
Electrophoresis of digested product [PSB1A3] Ar(EP),[B0015]Ar(XP),[PSB1K3]Kr(EP) (G1)
5/6 Mini-prep of [LacI] Ar (G3)
5/7 Transform of [PT7polymerase]Cr and [J61048]Ar (G2)
5/8 Transform of [B0015*3] Ar and [J61048*2] Ar (G2) Transform of [B0015] Ar and [J61048] Ar (G3) Transform of [B0015]Ar,[J61048]Ar,[J23101]Ar (G1) Mini-prep of [PT7polymerase]Cr, [T7polymerase*2]Cr and [B0015*2]Ar (G2) Digestion of [T7polymerase] Kr (EP) and [B0015] Ar (ES) (G2) Digestion of [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3) Electrophoresis of digested product of [Plac] Ar (ES), [mGFP] Ar (ES), [LacI] Ar (ES), [PSB1C3] Cr (EP) (G3)
5/9 Cultivation of [B0015] Ar, [J61048] Ar [LacI] Ar (G3)
5/10 Mini-prep of [LacI] Ar (G3)
5/11
Cultivation of [J61048] Ar (G2) (ES)
Electrophoresis of plasmid and digested product of [B0015]Ar(ES), [T7polymerase] Kr(only mini) and [T7promoter] Kr (EP)
Transform of [T7 promoter] Kr (G2)
5/12 Digestion of [T7polymerase] Kr (ES) and [J61048] Ar (ES) (G2)
5/13 Transform of [T7 promoter] Kr (G2)
5/15 Electrophoresis of plasmid and digested product of [J61048]Ar (ES), [T7polymerase*3]Kr(ES & XP) and [PSBIK3]Kr(EP) (G2)
Cultivation of [B0015]Ar, [J61048]Ar, [J23101]Ar, [PSB1K3]Kr (G1)
Mini-prep of [PSB1K3]Kr (G1)
Digestion of [PSB1K3]Kr (EP) (G1)
5/19
Cultivation of [J23101]Ar,[B0015]Ar,[J61048]Ar (G1)
5/20
Transform of [T7polymerase] Kr and [PSB1K3] Kr (G2)
Electrophoresis of plasmid and digested product of [T7polymerase*6] Kr and [PSB1K3*4]Kr (G2)
Mini-prep of [J23101]Ar, [B0015]Ar, [J61048]Ar (G1)
Digestion of [J23101]Ar (ES), [B0015]Ar (XP), [J61048]Ar (XP) (G1)
5/21
Digestion of [T7polymerase*6] Kr and [PSB1K3*2] Kr (G2)
Mini-prep of [T7polymerase] Kr and [PSB1K3] Kr (G2)
Electrophoresis of digested product and plasmid [J23101]Ar(ES), [B0015]Ar(XP), [J61048]Ar(XP) and digested product [PSB1K3]Kr(EP) (G1)
Ligation of [K592100+J61048+PSB1K3] Kr (G1)
5/22
Electrophoresis of plasmid and digested product of [T7polymerase] Kr (ES) and [PSB1K3]Kr (EP) (G2)
5/23
Transform of ligation product [K592100+J61048+PSB1K3]Kr (G1)
5/24
Digestion of [J61048] Ar (XP) (G2)
PCR of [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr (G2)
Electrophoresis of PCR products [BFP+B0032*4] Kr and [T7 promoter*1] Ar Kr, [J61048] Ar (G2)
5/27
Electrophoresis of digested product of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3)
Digestion of [J61048] Ar (XP) and [T7polymerase] Kr (ES) (G2)
Cultivation of [PSB1K3*3] Kr(G2)
5/28
PCR of [PSBIK3*5]Kr , [PSBIA3*5]Ar , [PSBIC3*5]Cr ,[J61048*5]Ar ,[B0015*5]Ar and [BFP*5](G1)
Mini-prep of [PSB1K3*3] Kr(G2)
Digestion of [PSB1K3*3] Kr (EP) (G2)
Electrophoresis of plasmid and digested product of [PSB1K3] Kr (EP), [T7polymerase] Kr (ES), [J61048] Ar (XP) (G2)
5/29
Digestion of [mGFP] Ar (ES), [J61048] Ar (XP) ,[B0015] Ar (XP) (G3)
Transform of [J23101] Ar (G1)
Transform of [T7 promoter] Ar Kr (G2)
Electrophoresis of plasmid and digested product of [J61048] Ar (ES), [T7polymerase] Kr (ES) (G2)
Ligation of [J61048+J61048] Cr, [T7polymerase+J61048] Kr, [RBS+BFP] Kr (G2)
Ligation of [Plac+B0032+PSB1K3] Kr (G3)
5/30
Transform of ligation product (G2)
Transform of ligation product [Plac+B0032+PSB1K3] Kr (G3)
