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Team:NCTU Formosa/Notebook/test
From 2014.igem.org
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<p> | <p> | ||
Transform of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr (G1)</p> | Transform of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr (G1)</p> | ||
| - | <p>4/12 | + | <p> |
| - | Cultivation of [PSB1K3] Kr, [PSB1C3] Cr (G2) | + | 4/12</p> |
| - | Cultivation of [PSB1A3] Ar, [PSB1C3] Cr (G1) | + | <p> |
| - | Cultivation of [mGFP] Ar (G3) | + | Cultivation of [PSB1K3] Kr, [PSB1C3] Cr (G2)</p> |
| - | </p> | + | <p> |
| + | Cultivation of [PSB1A3] Ar, [PSB1C3] Cr (G1)</p> | ||
| + | <p> | ||
| + | Cultivation of [mGFP] Ar (G3)</p> | ||
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
| - | <p>4/13 | + | <p> |
| - | Mini-prep of [mGFP] Ar, [PSB1K3] Kr(G3) | + | 4/13</p> |
| - | Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2) | + | <p> |
| + | Mini-prep of [mGFP] Ar, [PSB1K3] Kr (G3)</p> | ||
| + | <p> | ||
| + | Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2)</p> | ||
| + | <p> | ||
Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)</p> | Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)</p> | ||
| - | <p>4/17 | + | <p> |
| + | 4/17</p> | ||
| + | <p> | ||
Transform of [PSB1K3] Kr (G1)</p> | Transform of [PSB1K3] Kr (G1)</p> | ||
| - | <p>4/18 | + | <p> |
| + | 4/18</p> | ||
| + | <p> | ||
Cultivation of [PSB1K3] Kr (G1) </p> | Cultivation of [PSB1K3] Kr (G1) </p> | ||
| - | <p>4/19 | + | <p> |
| - | Transform of [Plac+PSB1A3] Ar, [PSB1K3] Kr , [PSB1A3] Ar, [PSB1C3] Cr (G3) | + | 4/19</p> |
| - | Mini-prep of [PSB1K3] Kr(G1) </p> | + | <p> |
| + | Transform of [Plac+PSB1A3] Ar, [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G3)</p> | ||
| + | <p> | ||
| + | Mini-prep of [PSB1K3] Kr (G1) </p> | ||
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
| - | <p>4/20 | + | <p> |
| - | Transform of [PSB1A3] Ar (G2) | + | 4/20</p> |
| - | Electrophoresis of [PSB1K3]Kr, [PSB1C3]Cr plasmid (G2)</p> | + | <p> |
| + | Transform of [PSB1A3] Ar (G2)</p> | ||
| + | <p> | ||
| + | Electrophoresis of [PSB1K3] Kr, [PSB1C3] Cr plasmid (G2)</p> | ||
| - | <p>4/21 | + | <p> |
| + | 4/21</p> | ||
| + | <p> | ||
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)</p> | Cultivation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)</p> | ||
| - | <p>4/22 | + | <p> |
| + | 4/22</p> | ||
| + | <p> | ||
Mini-prep of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)</p> | Mini-prep of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)</p> | ||
<br></div> | <br></div> | ||
<div class="single note"> | <div class="single note"> | ||
<p> | <p> | ||
| - | 4/28 | + | 4/28</p> |
| - | Electrophoresis of digested [PSB1K3] Kr(EP), [PSB1C3] Cr(EP),[PSB1A3] Ar(EP) (G1) | + | <p> |
| - | Cultivation of [PSB1C3] Cr (G1) | + | Electrophoresis of digested [PSB1K3] Kr(EP), [PSB1C3] Cr(EP),[PSB1A3] Ar(EP) (G1)</p> |
| - | </p> | + | <p> |
| + | Cultivation of [PSB1C3] Cr (G1)</p> | ||
| + | <p> | ||
| + | 4/29</p> | ||
| + | <p> | ||
| + | Mini-prep of [PSB1C3] Cr (G1)</p> | ||
| + | <p> | ||
| + | Mini-prep of [PSB1C3] Cr (G2)</p> | ||
| + | <p> | ||
| + | Transform of [B0032] Ar, and [BFP] Cr (G2)</p> | ||
| + | <p> | ||
| + | Cultivation of [PSB1K3] Kr, [PSB1A3] Ar (G1)</p> | ||
| + | <p> | ||
| + | Cultivation of [B0032] Ar, and [BFP] Cr (G2)</p> | ||
| + | <p> | ||
| + | 4/30</p> | ||
| + | <p> | ||
| + | Mini-prep of [PSB1A3] Ar (G1)</p> | ||
| + | <p> | ||
| + | Mini-prep of [B0032] Ar, and [BFP] Cr (G2)</p> | ||
| + | <p> | ||
| + | Transform of [B0032] Ar, and [BFP] Cr, [T7promoter] Ar Kr and [T7 polymerase] Cr (G2)</p> | ||
| + | <p> | ||
| + | Transform of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)</p> | ||
<p> | <p> | ||
| - | + | Digestion of [PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)</p> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | </p> | + | |
<p> | <p> | ||
| - | + | Electrophoresis of plasmid and digested prouct of [PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)</p> | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | Electrophoresis of plasmid and digested prouct of [PSBIC3]Cr(EP), [B0032]Ar(ES), [BFP]Cr(XP) mini (G2) | + | |
| - | </p> | + | |
<br></div> | <br></div> | ||
Revision as of 12:44, 13 October 2014
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4/11
Transform of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
Transform of [Plac+PSB1A3] Ar, [mGFP+PSB1A3] Ar (G3)
Transform of [PSB1K3] Kr,[PSB1A3] Ar, [PSB1C3] Cr (G1)
4/12
Cultivation of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Cultivation of [PSB1A3] Ar, [PSB1C3] Cr (G1)
Cultivation of [mGFP] Ar (G3)
4/13
Mini-prep of [mGFP] Ar, [PSB1K3] Kr (G3)
Mini-prep of [PSB1K3] Kr, [PSB1C3] Cr (G2)
Mini-prep of [PSB1A3] Ar, [PSB1C3] Cr (G1)
4/17
Transform of [PSB1K3] Kr (G1)
4/18
Cultivation of [PSB1K3] Kr (G1)
4/19
Transform of [Plac+PSB1A3] Ar, [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G3)
Mini-prep of [PSB1K3] Kr (G1)
4/20
Transform of [PSB1A3] Ar (G2)
Electrophoresis of [PSB1K3] Kr, [PSB1C3] Cr plasmid (G2)
4/21
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/22
Mini-prep of [PSB1K3] Kr, [PSB1A3] Ar, [PSB1C3] Cr (G2)
4/28
Electrophoresis of digested [PSB1K3] Kr(EP), [PSB1C3] Cr(EP),[PSB1A3] Ar(EP) (G1)
Cultivation of [PSB1C3] Cr (G1)
4/29
Mini-prep of [PSB1C3] Cr (G1)
Mini-prep of [PSB1C3] Cr (G2)
Transform of [B0032] Ar, and [BFP] Cr (G2)
Cultivation of [PSB1K3] Kr, [PSB1A3] Ar (G1)
Cultivation of [B0032] Ar, and [BFP] Cr (G2)
4/30
Mini-prep of [PSB1A3] Ar (G1)
Mini-prep of [B0032] Ar, and [BFP] Cr (G2)
Transform of [B0032] Ar, and [BFP] Cr, [T7promoter] Ar Kr and [T7 polymerase] Cr (G2)
Transform of [J23101] Ar,[K592100] Cr,[B0032] Ar,[B0015] Ar (G1)
Digestion of [PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
Electrophoresis of plasmid and digested prouct of [PSBIC3] Cr(EP), [B0032] Ar(ES), [BFP] Cr(XP) mini (G2)
