Team:TCU Taiwan/Attributions

From 2014.igem.org

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               <td width="50%"><font size="3" face="Verdana" color="#333"><li>Transformation ( Cas9 in  pSB1C3 into DH5α)<br>
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               <td width="50%" valign="top"><font size="3" face="Verdana" color="#333"><li>Transformation ( Cas9 in  pSB1C3 into DH5α)<br>
                 <li>Extract plasmid with pSB1C3  from DH5α.<br>
                 <li>Extract plasmid with pSB1C3  from DH5α.<br>
                 <li>Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011.<br>
                 <li>Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011.<br>
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               <td colspan="2"><font size="3" face="Verdana" color="#0092C7">House, Bobo, Chao-Di CHANG(National Chiao Tung University)</font></td>
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               <td colspan="2"><font size="3" face="Verdana" color="#0092C7">House, Bobo, Chao-Di Chang(National Chiao Tung University)</font></td>
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               <td width="50%"><font size="3" face="Verdana" color="#333"><li>Infectional efficiency ratio of  M13KO7<br>
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               <td width="50%" valign="top"><font size="3" face="Verdana" color="#333"><li>Infectional efficiency ratio of  M13KO7<br>
                 <li> Release titer(Releasing titer of phagemid which is E.coli infected by helper phage)<br>
                 <li> Release titer(Releasing titer of phagemid which is E.coli infected by helper phage)<br>
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               <td width="50%"><font size="3" face="Verdana" color="#333"><li>Held the 2014 Synthetic Biology information meeting of local senior high school and Tzu Chi University as a chief convener.<br>
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               <td width="50%" valign="top"><font size="3" face="Verdana" color="#333"><li>Held the 2014 Synthetic Biology information meeting of local senior high school and Tzu Chi University as a chief convener.<br>
                 <li>Contact with National Chiao Tung University for cooperation.<br></font></td>
                 <li>Contact with National Chiao Tung University for cooperation.<br></font></td>
               <td rowspan="3">&nbsp;</td>
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              <td height="30px">&nbsp;</td>
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              <td>&nbsp;</td>
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              <td bgcolor="#FFF2B5"  height="20px" colspan="2"><font face="Trebuchet MS" size="6" color="#A66B38">Special Thanks</font></td>
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              <td colspan="2"><font size="3" face="Verdana" color="#0092C7">Our partner : NCTU_Formosa</font>
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                <li>We are extremely appreciative of NCTU_Formosa that helped and support us.<br>
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                <li>Modeling courtesy of Chao-Di Chang.<br></font></td>
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              <td rowspan="3" align="center"><a href="https://2014.igem.org/Team:NCTU_Formosa" target="_blank" title="NCTU_Formosa Wiki"><img src="https://static.igem.org/mediawiki/2014/0/09/FINAL.gif" width="90%"></td>
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Revision as of 00:12, 13 October 2014


 
Attributions
  
We would like to thank all the people who came to our progress presentations and gave useful feedback.
 
Experiment
 
Bobo, Regina, Peiyi, Eating
 
Contributions
 
  • Transformation ( Cas9 in pSB1C3 into DH5α)
  • Extract plasmid with pSB1C3 from DH5α.
  • Extract plasmid pSB1C3 with BBa_I13521、BBa_ K914003、BBa_ K1218011.
  • Use Enzyme digestion (PstI and EcoRI) to confirm whether pSB1C3 and pBluescript is successfully extracted.
  • Purification of RFP sequence from pSB1C3 Prepare Cm. plate、LB. plate.
  • Ligation of RFP and pBluescript SK(-).
  • Transformation clone RFP/pBluescript SK(-) to DH5α.
  • Extract plasmid clone RFP/pBluescript SK(-).
    		•   
    Experiment Design

    Ted and Bobo

    Design gRNA for AmpR.

    Biobricks Design

    Ted

     Design the gRNA for different resistance gene.

       
    Modeling
     
    House, Bobo, Chao-Di Chang(National Chiao Tung University)
     
    Contributions
     
  • Infectional efficiency ratio of M13KO7
  • Release titer(Releasing titer of phagemid which is E.coli infected by helper phage)
    		•   
       
    Human Practice
     
    Eating
     
    Contributions
     
  • Held the 2014 Synthetic Biology information meeting of local senior high school and Tzu Chi University as a chief convener.
  • Contact with National Chiao Tung University for cooperation.
    		•   
       
    And last but not least
    Art Design

    Peiyi, Regina, Eating, House

    Design of poster and PPT.
    Design of team uniform.

    		 
    Photo & Video

    Sharon, Eating

     Take photo and video.
    Arrange all photos.

    Administeration

    Eating

    Apply for the allowances.
    Arrange all files.

    		  
    Wiki

    House

    Coding,coding and coding the wiki page.
       
    Special Thanks
     
    Our partner : NCTU_Formosa
     
  • We are extremely appreciative of NCTU_Formosa that helped and support us.
  • Modeling courtesy of Chao-Di Chang.
    		•
       

    iGEM Team attributions page

    Each team must clearly attribute work done by the student team members on this page. The team must distinguish work done by the students from work done by others, including the host labs, advisors, instructors, and individuals not on the team roster.

    Why do we have this requirement?

    Attribution Template

    We have this requirement to help the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, just be sure to report the work your team did and the work that was done by others.

    For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is something that is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.

    A great example of complete attribution comes from the Imperial College London 2011 team (scroll down to the bottom of their team page to see attributions).

    Here are some of the fields we recommend you have on this page. If there are other areas not listed below, but applicable to your team/project, please feel free to also list them on your attributions page. Please feel free to remove any areas not applicable to your project.

    1. General Support
    2. Project support and advice
    3. Fundraising help and advice
    4. Lab support
    5. Difficult technique support
    6. Project advisor support
    7. Wiki support
    8. Presentation coaching
    9. Policy & Practices support
    10. Thanks and acknowledgements for all other people involved in helping make a successful iGEM team.
    ^


        
    Team Members Project Parts Human Pratics Modeling Safety Notebook Attributions

    Lost the way? Use it to help you if you're lost.

    ^

    Retrieved from "http://2014.igem.org/Team:TCU_Taiwan/Attributions"