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Team:TU Eindhoven/Modeling/Cell Encapsulation Modeling
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<p>Based on these results a lambda of 0.5 was chosen for the microfluidic device. The Poisson distribution for different number of cells becomes then as follows.</p> | <p>Based on these results a lambda of 0.5 was chosen for the microfluidic device. The Poisson distribution for different number of cells becomes then as follows.</p> | ||
| - | <img id='Fig3' src="https://static.igem.org/mediawiki/2014/ | + | <img id='Fig3' src="https://static.igem.org/mediawiki/2014/9/91/TU_Eindhoven_Modeling3.png" class="image_wrapper image_fr" width="1085"> |
| - | <p style="font-size:18px;color:#CCCCCC;">Figure 3.</p> | + | <p style="font-size:18px;color:#CCCCCC;">Figure 3. Modeled Poisson distribution for a specific lambda of 0.3 together with vs. the experimental Poisson distribution based on a lambda of 0.3.</p> |
<h4>Bibliography</h4> | <h4>Bibliography</h4> | ||
| - | <p></p> | + | <p>Mazutis, Linas, John Gilbert, W Lloyd Ung, David A Weitz, Andrew D Griffiths and John A Heyman. Single-cell analysis and sorting using droplet-based microfluidics. Nature protocols 8.5 (2013): 870-891.</p> |
</div> | </div> | ||
Revision as of 17:49, 12 October 2014
Cell Encapsulation Modeling
The modeling group focused on the microfluidics. With microfluidics the cells are separated into a single droplet. This process however is not very accurate. Sometimes the droplets do not contain the desired number of cells. This can be modelled using a Poisson distribution. In this model you can define an average number of cells per droplet, lambda. Lambda was varied to choose a suitable average number of cells per droplet for our microfluidic device.
Figure 1. Poisson distribution for multiple lambdas.
This allows for the determination of the ratios between one cell/zero cells and one cell/ two or more cells. These both have to be as high as possible for a specific lambda.
Figure 2. Ratio between one cell/ zero cells or more cells
Based on these results a lambda of 0.5 was chosen for the microfluidic device. The Poisson distribution for different number of cells becomes then as follows.
Figure 3. Modeled Poisson distribution for a specific lambda of 0.3 together with vs. the experimental Poisson distribution based on a lambda of 0.3.
Bibliography
Mazutis, Linas, John Gilbert, W Lloyd Ung, David A Weitz, Andrew D Griffiths and John A Heyman. Single-cell analysis and sorting using droplet-based microfluidics. Nature protocols 8.5 (2013): 870-891.
