Team:EPF Lausanne/Data

From 2014.igem.org

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Data For Our Favorite New Parts</br></br>
Data For Our Favorite New Parts</br></br>
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</br>
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<p>Documented & submitted:
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<ul>
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<li>BBa_K1486001 (Ara CpxR http://parts.igem.org/Part:BBa_K1486001 ) </li>
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<li>BBa_K1486002 (Ara GFPCpxR http://parts.igem.org/Part:BBa_K1486002 )</li>
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<li>BBa_K1486005 (Ara CpxrGFP http://parts.igem.org/Part:BBa_K1486005 )</li>
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<li>BBa_K1486049 (CpxR reporter promoter FW http://parts.igem.org/Part:BBa_K1486049 )</li>
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<li>BBa_K1486050 (CpxR reporter promoter RV http://parts.igem.org/Part:BBa_K1486050 )</li>
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</ul>
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</p>
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<p>Works as expected:
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<ul>
 +
<li>BBa_K1486002 (Ara GFPCpxR http://parts.igem.org/Part:BBa_K1486002 )</li>
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<li>BBa_K1486005 (Ara CpxRGFP http://parts.igem.org/Part:BBa_K1486005 )</li>
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<li>BBa_K1486008 (GA1 TedCécile http://parts.igem.org/Part:BBa_K1486008 )</li>
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<li>BBa_K1486018 (Ara split fLuc http://parts.igem.org/Part:BBa_K1486018 )</li>
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<li>BBa_K1486021 (Ara split rLuc http://parts.igem.org/Part:BBa_K1486021 )</li>
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</ul></p>
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<p>Works as expected & submitted:
 +
<ul>
 +
<li>BBa_K1486002 (Ara GFPCpxR http://parts.igem.org/Part:BBa_K1486002 )</li>
 +
<li>BBa_K1486005 (Ara CpxrGFP http://parts.igem.org/Part:BBa_K1486005 )</li>
 +
<li>BBa_K1486018 (Ara split fLuc http://parts.igem.org/Part:BBa_K1486018 )</li>
 +
<li>BBa_K1486021 (Ara split rLuc http://parts.igem.org/Part:BBa_K1486021 )</li>
 +
<li>BBa_K1486049 or BBaK1486050 (CpxR reporter promoter FW or RV http://parts.igem.org/Part:BBa_K1486050 )</li>
 +
</ul></p>
 +
</br></br></br>
Data For Pre-existing Parts</br></br>
Data For Pre-existing Parts</br></br>
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</br></br></br></br>
+
</br>
 +
<p>Documented & submitted: BBa_K1486056 (CpxR reporter (Calgary) http://parts.igem.org/Part:BBa_K1486056 ) We repaired it</p>
 +
</br></br></br>
We've Also Characterized the Following Parts</br></br>
We've Also Characterized the Following Parts</br></br>
</br></br></br></br>
</br></br></br></br>
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<h4><b>General Objective: Develop a fast and specific biosensor allowing spatiotemporal analysis of stimuli</b></h4>
 
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To achieve the objective mentioned above, we put forward the following hypothesis:
 
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<div class="cntr">
 
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<u>Hypothesis:</u> Combining protein complementation techniques (split IFP1.4) to proteins going through dimerization (CpxR) upon activation due to a given stimuli (enveloppe stress) allows fast and specific spatiotemporal analysis of a stimuli.
 
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To validate this hypothesis we set ourselves three intermediate objectives:
 
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<u>First intermediate objective:</u> Explore if and how CpxR dimerizes in live E.Coli by analyzing the various orientations of CpxR - IFP1.4 fusions.
 
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<u>Second intermediate objective:</u> Analyse the temporal dynamics of the functional CpxR - IFP1.4 fusion protein by change of media.
 
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<u>Third intermediate objective:</u> Analyse the spatial dynamics of the functional CpxR - IFP1.4 fusion protein by microscopy.
 
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To validate these three objectives, we designed three experiments:
 
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<u>First Experiment:</u> Build constructs with all the possible orientations between CpxR and IFP1.4 and monitor activation upon 50mM KCl stress activation
 
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<u>Second Experiment:</u> Induce signal activation by stressing C-termina CpxR - split IFP1.4 constructs with 50 mM KCl, and subsequently replacing the media with a 0 mM KCl solution
 
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<u>Third Experiment:</u> Analyse the spatial dynamics of the functional CpxR - IFP1.4 fusion protein by microscopy.
 
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<!-- END ABSTRACT -->

Revision as of 11:56, 12 October 2014

DATA



Bacterial Biosensors



Exp_IFP


Data For Our Favorite New Parts


Documented & submitted:

  • BBa_K1486001 (Ara CpxR http://parts.igem.org/Part:BBa_K1486001 )
  • BBa_K1486002 (Ara GFPCpxR http://parts.igem.org/Part:BBa_K1486002 )
  • BBa_K1486005 (Ara CpxrGFP http://parts.igem.org/Part:BBa_K1486005 )
  • BBa_K1486049 (CpxR reporter promoter FW http://parts.igem.org/Part:BBa_K1486049 )
  • BBa_K1486050 (CpxR reporter promoter RV http://parts.igem.org/Part:BBa_K1486050 )

Works as expected:

  • BBa_K1486002 (Ara GFPCpxR http://parts.igem.org/Part:BBa_K1486002 )
  • BBa_K1486005 (Ara CpxRGFP http://parts.igem.org/Part:BBa_K1486005 )
  • BBa_K1486008 (GA1 TedCécile http://parts.igem.org/Part:BBa_K1486008 )
  • BBa_K1486018 (Ara split fLuc http://parts.igem.org/Part:BBa_K1486018 )
  • BBa_K1486021 (Ara split rLuc http://parts.igem.org/Part:BBa_K1486021 )

Works as expected & submitted:

  • BBa_K1486002 (Ara GFPCpxR http://parts.igem.org/Part:BBa_K1486002 )
  • BBa_K1486005 (Ara CpxrGFP http://parts.igem.org/Part:BBa_K1486005 )
  • BBa_K1486018 (Ara split fLuc http://parts.igem.org/Part:BBa_K1486018 )
  • BBa_K1486021 (Ara split rLuc http://parts.igem.org/Part:BBa_K1486021 )
  • BBa_K1486049 or BBaK1486050 (CpxR reporter promoter FW or RV http://parts.igem.org/Part:BBa_K1486050 )




Data For Pre-existing Parts


Documented & submitted: BBa_K1486056 (CpxR reporter (Calgary) http://parts.igem.org/Part:BBa_K1486056 ) We repaired it




We've Also Characterized the Following Parts





Sponsors

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