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Team:Hannover/Protocols/EasyIsolation
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| - | <h1>A simple method for DNA isolation</h1> | + | <h1><a href="https://2014.igem.org/Team:Hannover/Protocols" target="_blank">Protocols</a> / A simple method for DNA isolation</h1> |
<p class="text">A lot of people don’t know that our food contains genetic material. Therefor we performed a DNA isolation from bananas with housekeeping articles to educate interested passerby.</p> | <p class="text">A lot of people don’t know that our food contains genetic material. Therefor we performed a DNA isolation from bananas with housekeeping articles to educate interested passerby.</p> | ||
<table style="float:right"><tr><td style="border:0px"><a href="https://static.igem.org/mediawiki/2014/a/ad/Hannover_20141003_Sbd6_klein.jpg | <table style="float:right"><tr><td style="border:0px"><a href="https://static.igem.org/mediawiki/2014/a/ad/Hannover_20141003_Sbd6_klein.jpg | ||
Revision as of 11:53, 12 October 2014
Protocols / A simple method for DNA isolation
A lot of people don’t know that our food contains genetic material. Therefor we performed a DNA isolation from bananas with housekeeping articles to educate interested passerby.
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Material: |
|
| Bananas | Tap water |
| Table salt | Dishwashing liquid as detergent |
| Washing agent with proteases | Hot water (60 °C) |
| Ice | Teaspoon |
| Tablespoon | Fork |
| Isopropyl (alcohol) (cooled) | Coffee filter |
| Funnel | 50 mL vessels |
Test execution:
- preparation of extraction solution:
- 1 teaspoon table salt
- 1 tablespoon dishwashing liquid
- 10 drops washing agent
- Fill up to 100 mL with tap water
- Mixing by shaking
- Homogenization of a banana with the fork
- Transfer of 3 teaspoons homogenized banana into a 50mL reaction vessel with 30 mL extraction solution.
- 15 min incubation at 60 °C
- 5 min chilling on ice
- Filter the mixture into a new 50 mL vessel.
- Overlay the filtered solution with some iosporyl (alcohol)
- Wait for 10 min
- DNA is soaring into the phase of isopropyl (alcohol)
