"
Team:HokkaidoU Japan/Notebook/Length/as90 120
From 2014.igem.org
(Difference between revisions)
(Created page with "{Team:HokkaidoU_Japan/CSS}} {{Team:HokkaidoU_Japan/Notebook/CSS}} <html> <div class="wrapper"> <div id="hokkaidou-contents"> <h2> Anti-sense 90, 120 construction ...") |
|||
| Line 1: | Line 1: | ||
| - | {Team:HokkaidoU_Japan/CSS}} | + | {{Team:HokkaidoU_Japan/CSS}} |
| - | + | {{Team:HokkaidoU_Japan/Notebook/CSS}} | |
<html> | <html> | ||
<div class="wrapper"> | <div class="wrapper"> | ||
Revision as of 11:39, 12 October 2014
Anti-sense 90, 120 construction
-
Start
-
PCR & PCR purification
-
R0040-B0034-E1010-B0015 as RBS XhoI, 90bp as mRFP Nco as RBS XhoI, 120bp as mRFP Nco
-
Digestion
-
Cut as 90, as120, H-stem with NcoI, XhoI(using 10×Cut Smart)
-
Ethanol precipitation
-
as90, as120
-
Gel Extraction
-
H-stem
-
Dephophorylation
-
H-stem
-
Ligation
-
Ligate as90, as120 with H-stem
-
Transformation
-
as90, as120 on H-stem 5µL DNA to DH5α Turbo
-
Colony PCR
-
as90, as120 on H-stem
-
Liquid Culture
-
as90, as120 on H-stem
-
Mini-prep
-
as90, as120 on H-stem
-
Dye terminator
-
as90, as120 on H-stem
-
Complete!
