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Team:ETH Zurich/project/overview/implementationsimple
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*For sensing the signals coming from above, we added in every cell two genes (''luxR'' and ''lasR'') that produce two proteins (LuxR and LasR) that will bind respectively the blue and the red quorum sensing molecules. The blue and red complexes created this way trigger the production of other proteins called integrases (Bxb1 and ΦC31). | *For sensing the signals coming from above, we added in every cell two genes (''luxR'' and ''lasR'') that produce two proteins (LuxR and LasR) that will bind respectively the blue and the red quorum sensing molecules. The blue and red complexes created this way trigger the production of other proteins called integrases (Bxb1 and ΦC31). | ||
| - | * A gene sequence is placed that blocks expression of the fluorescent protein. You can see in the animation that if an integrase is present, it can remove this blocking sequence. However if both integrases are present, this sequence is flipped twice and it blocks production of fluorescence again. | + | * A gene sequence is placed that blocks expression of the fluorescent protein (see the large, black T in the animation above). You can see in the animation that if an integrase is present, it can remove this blocking sequence (turn the black T upside down). However if both integrases are present, this sequence is flipped twice and it blocks production of fluorescence again. |
*When the fluorescent protein is produced, an enzyme is also produced which triggers the production of a quorum sensing molecule sent to the cells below. | *When the fluorescent protein is produced, an enzyme is also produced which triggers the production of a quorum sensing molecule sent to the cells below. | ||
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If you want to know more about quorum sensing and integrases, you can read the article [[Team:ETH_Zurich/project/background#Biotools|Biological tools]] in our Background page. | If you want to know more about quorum sensing and integrases, you can read the article [[Team:ETH_Zurich/project/background#Biotools|Biological tools]] in our Background page. | ||
Revision as of 10:11, 12 October 2014
More details
E. Coli bacteria are able to communicate together by producing molecules that can penetrate their cell membrane. These molecules are called quorum sensing molecules. In our project Mosaicoli, every cell colony on the grid is able to sense these molecules coming from the two colonies above it, and to produce a molecule for the next colonies below it.In order to make a pattern appear on our grid, we need to tell every cell on this grid:
- to sense the signals coming from the 2 cells above.
- if it senses only one signal, to produce a fluorescent protein and a signal towards the cells below
- to produce nothing if it senses both signals, or if it doesn't sense any signal.
In synthetic biology, you can tell the cell to compute this algorithm by inserting genes into it. This is how we did it:
- For sensing the signals coming from above, we added in every cell two genes (luxR and lasR) that produce two proteins (LuxR and LasR) that will bind respectively the blue and the red quorum sensing molecules. The blue and red complexes created this way trigger the production of other proteins called integrases (Bxb1 and ΦC31).
- A gene sequence is placed that blocks expression of the fluorescent protein (see the large, black T in the animation above). You can see in the animation that if an integrase is present, it can remove this blocking sequence (turn the black T upside down). However if both integrases are present, this sequence is flipped twice and it blocks production of fluorescence again.
- When the fluorescent protein is produced, an enzyme is also produced which triggers the production of a quorum sensing molecule sent to the cells below.
If you want to know more about quorum sensing and integrases, you can read the article Biological tools in our Background page.
