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Team:UT-Tokyo/CTCD/Content
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<legend>Fig.3 the construct of our circuit</legend> | <legend>Fig.3 the construct of our circuit</legend> | ||
<h3>EGP-2 promoter</h3> | <h3>EGP-2 promoter</h3> | ||
| - | <p>EGP-2 promoter is the promoter of Epithelial cell adhesion molecule (EpCAM) protein. | + | <p>EGP-2 promoter is the promoter of Epithelial cell adhesion molecule (EpCAM) protein.EpCAM is a trans membrane glycoprotein which is expressed in epithelial cells and shows high level expression in various type of human epithelial carcinomas[2].Using this promoter, reporter genes can be expressed only in cancer cells. EGP-2 promoter is originally about 3500bp and it is not convenient to make a DNA construct. But, in the previous research, it is known that EGP-2 promoter can work if a part of its sequence is deleted[3]. In this project ,We cloned a part of EGP-2 promoter, and made it useful as a biobrick parts.</p> |
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<h3>miRNA-142</h3> | <h3>miRNA-142</h3> | ||
| - | <p>MiRNA is a class of non-coding RNA.MiRNA binds the miRNA-recognition element in the 3' untranslated region of the target gene. MiRNA shows the tissue-specific expression pattern. In our project, we use miRNA-142, which is expressed only in hematopoietic cells[ | + | <p>MiRNA is a class of non-coding RNA.MiRNA binds the miRNA-recognition element in the 3' untranslated region of the target gene. MiRNA shows the tissue-specific expression pattern. In our project, we use miRNA-142, which is expressed only in hematopoietic cells[4].After miRNA-142 is transcribed, it is cleaved to miRNA-142-5p and miRNA-142-3p. Adding the recognition element of miRNA-142 in the 3' untranslated region of the reporter genes, leaky expression in non-carcinoma hematopoietic cells can be reduced.</p> |
<img src="https://static.igem.org/mediawiki/2014/9/94/Kobari_CTC.png" class = "figure" /> | <img src="https://static.igem.org/mediawiki/2014/9/94/Kobari_CTC.png" class = "figure" /> | ||
<legend>Fig.4 When this construct is transfected to the epithelial cells like cancer cell, pEGP-2 promote the transcription of EGFP, and the mRNA is not degraded by miRNA because miR 142 is specific to hematopoietic cells.</legend> | <legend>Fig.4 When this construct is transfected to the epithelial cells like cancer cell, pEGP-2 promote the transcription of EGFP, and the mRNA is not degraded by miRNA because miR 142 is specific to hematopoietic cells.</legend> | ||
Revision as of 09:58, 12 October 2014
