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| - | <center><groupparts>iGEM14 ULB-Brussels</groupparts></center> | + | <br/><br/><center><groupparts>iGEM14 ULB-Brussels</groupparts></center> |
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| - | <p class="title"><font color="#002B9B">
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| - | $The$ $Mighty$ $Coli$ $BioBrick$
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| - | <section style="margin: -20px"></section>
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| - | <h1> Our Part </h1>
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| - | <br>
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| - | $\Longrightarrow$ <a href="http://parts.igem.org/Part:BBa_K1318000"> ULB-Brussels part </a>
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| - | <section style="margin: 20px"></section>
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| - | <h3> Properties </h3>
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| - | <p> Name: BBa_K1318000 <br>
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| - | Host: E.coli <br>
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| - | Source: ccdBA operon <br>
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| - | Plasmid: available using <i>LabGenius BioBrick Mapper</i> <br>
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| - | Length: 309 bp <br>
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| - | Sequence: (begin) atgcagttt ... atataataa (end) <br>
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| - | Compatibility: RFC[10], RFC[12], RFC[21], RFC[23], RFC[25]. <br>
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| - | </p>
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| - | </section>
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| - | <h3> Characterization </h3>
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| - | <p> In order to characterize the ccdB biobrick, we sent the biobricks to sequencing and made a <i>screen of activity </i> for the protein ccdB. We did a killing assay, because of the toxic property of ccdB.<br><!--$\small killing$ $\small assay$--><br>
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| - | We constructed 4 different colonies including one with the plasmid pKK233/ccdA, another with pBAD33/ccdB, a third with both, and a control colony. The ccdA gene encoded for a protein wich acts as an anti-toxin of ccdB.<br><!--$\small\hspace{0.08cm} 4$ $\small different$ $\small colonies\hspace{0.06cm}$ --><br>
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| - | On the first media containing <i>IPTG</i> (inducing the pKK233’s expression) <i>and glucose</i> (repressing the pBAD's expression), each colony grew. That allowed us to control the non toxicity of ccdA.
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| - | On the media containing both <i>IPTG and arabinose</i> (inducing the pBAD's expression), the strand with pBAD was killed and the strand with both ccdA $\small\&$ ccdB grew.<br><br>
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| - | We made <i>dilutions</i> to assure that the cell concentration didn’t affect the toxicity or the anti-toxicity. </p>
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| - | </section><section style="margin: -15px"></section>
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| - | <section style="text-align: left">
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| - | <a href="https://2014.igem.org/Team:ULB-Brussels/Parts"> < Previous Bricks </a>
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| - | <section style="margin: 15px"></section>
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| - | </section></tr>
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| | </table><br/><br/> | | </table><br/><br/> |
$~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
\newcommand{\MyColi}{{\small Mighty\hspace{0.12cm}Coli}}
\newcommand{\Stabi}{\small Stabi}$
$\newcommand{\EColi}{\small E.coli}
\newcommand{\SCere}{\small S.cerevisae}\\[0cm]
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
\newcommand{\PI}{\small PI}$
$\newcommand{\Igo}{\Large\mathcal{I}}
\newcommand{\Tgo}{\Large\mathcal{T}}
\newcommand{\Ogo}{\Large\mathcal{O}}
~$
"
- Université Libre de Bruxelles -
