"
Team:Gifu/Parts
From 2014.igem.org
| Line 133: | Line 133: | ||
</body> | </body> | ||
</html> | </html> | ||
| - | |||
| - | |||
<!--</div>--> | <!--</div>--> | ||
Revision as of 02:19, 12 October 2014
Parts Submitted to the Registry
An important aspect of the iGEM competition is the use and creation of standard biological parts. Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.
Note that if you want to document a part you need to document it on the Registry, not on your team wiki. Future teams and other users and are much more likely to find parts on the Registry than on your team wiki.
Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without a need to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.
When should you put parts into the Registry?
As soon as possible! We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better recall you will have of all details surrounding your parts. Remember you don't need to send us the DNA to create an entry for a part on the Registry. However, you must send us the sample/DNA before the Jamboree. Only parts for which you have sent us samples/DNA are eligible for awards and medal requirements.
What information do I need to start putting my parts on the Registry?
The information needed to initially create a part on the Registry is:
- Part Name
- Part type
- Creator
- Sequence
- Short Description (60 characters on what the DNA does)
- Long Description (Longer description of what the DNA does)
- Design considerations
We encourage you to put up much more information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. Check out part BBa_K404003 for an excellent example of a highly characterized part.
You can add parts to the Registry at our Add a Part to the Registry link.
Parts Table
Any parts your team has created will appear in this table below:
Parts
Histidine tag (8 AA) and RFP
This part codes for a RFP. A histidine tag is attached to a RFP that is coded by this part. So the RFP can be purified by immobilized metal ions affinity chromatography.
Histidine tag (8 AA) and RFP (without stop codon)
A Histidine tag is attached to a RFP that is coded by this part. So the RFP can be purified by immobilized metal ions affinity chromatography.
!!Caution!!
A stop codon in this part is removed. Therefore, this part doesn’t have an ability of a usual protein coding part. The combination of this part and the mRNA circularization device is capable of generating the RFP (+histidine tag) polymer. The part to generate it is registered ([http://parts.igem.org/Part:BBa_K1332011 BBa_K1332011]).
The 5´ side of the intron from td gene of T4 phage without stop codon
This part is a part of the mRNA circularization device (3’ side). A stop codon in this part removed. The combination of this part and a terminator is the mRNA circularization device (3’ side) (BBa_K1332009).
The 5´ side of the intron from td gene of T4 phage
This part is a part of the mRNA circularization device (3’ side). The combination of this part and a terminator is the mRNA circularization device (3’ side) (BBa_K1332010). But there are a stop codon in this part, so a circular mRNA made by BBa_K1332010 (mRNA circularization device (3’ side); It consist of this part and double terminator.) doesn’t cause continuous translation.
The 3´ side of the intron from td gene of T4 phage
This part is a part of the mRNA circularization device (5’ side). The combination of this part, a promoter (lacI regulated) (BBa_R0010) and RBS (BBa_B0034) is the mRNA circularization device (5’ side) (BBa_K1332008).
mRNA circularization device (5´ side)
This part consists of a promoter (lacI regulated), the 3´ side of the intron in td gene of T4 phage and RBS. The protein coding sequence that is inserted between this device and mRNA circularization device (3’ side) can be circularized. If you circularized the protein coding sequence (Its a stop codon have been removed.) with mRNA circularization device (3’ side) for endless translation, you can get a circular mRNA that is translated semi-permanently.
mRNA circularization device (3´ side) (endless translation)
This part consists of the 5´ side of the intron in td gene of T4 phage and a double terminator. The protein coding sequence that is inserted between this device and mRNA circularization device (5’ side) can be circularized. If you circularized the protein coding sequence (It’s a stop codon have been removed.), you can get a circular mRNA that is translated semi-permanently.
mRNA ircularization device (3´ side)
This part consists of the 5´ side of the intron in td gene of T4 phage and a double terminator. The protein coding sequence that is inserted between this device and mRNA circularization device (5´ side) can be circularized. The 5´side of the intron in td gene of T4 phage contains a stop codon, so a circular mRNA made by this part doesn’t cause continuous translation.
Histidine tag (8 AA) and RFP semi-permanent generator
This generator is capable of synthesizing a RFP (+histidine tag) polymer. This generator consists of a circularization device (5´ side), histidine tag (8 AA) and RFP (without stop codon) and a circularization device (3´ side). A mRNA is circular, so translation continues semi-permanently. A synthesis of the RFP (+histidine tag) become possible by a simply transformation, but the coloration of RFP is weak.
TTHA0715 with sequence cut by thrombin semi-permanent generator
This generator is capable of synthesizing TTHA0715 (with amino acid sequence cut by thrombin). This generator consists of a circularization device (5´ side), TTHA0715 (with amino acid sequence cut by thrombin) and a circularization device (3´ side). A mRNA is circular, so translation continues semi-permanently. TTHA0715 is a cold shock protein and ABC transporter ATP-binding protein. The use of this device is to confirm the existence of the long-chain protein that is made by a circular mRNA. But this part didn’t work.
SmtA semi-permanent generator
This generator is capable of synthesizing SmtA (BBa_K519010). This generator consists of an mRNA circularization device (´ side), SmtA (BBa_K519010) and an mRNA circularization device (3´ side). An mRNA is circular, so translation continues semi-permanently. SmtA is a metallothionein that can catch heavy metal ions such as Zn2+.
